公开(公告)号：US07745671B2

公开(公告)日：2010-06-29

申请号：US10576030

申请日：2004-10-14

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C07C35/16 ,  C07H1/06 ,  C07C35/46

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌内糖; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸络合物，将所述络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US20130196417A1

公开(公告)日：2013-08-01

申请号：US13791172

申请日：2013-03-08

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C12N9/00

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌内糖; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸复合物，将络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US08409833B2

公开(公告)日：2013-04-02

申请号：US12712635

申请日：2010-02-25

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C12P19/02

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌内糖; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸络合物，将络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US20060240534A1

公开(公告)日：2006-10-26

申请号：US10576030

申请日：2004-10-14

申请人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

发明人： Masanori Yamaguchi ,  Yuichi Kita ,  Tetsuya Mori ,  Kenji Kanbe ,  Akihiro Tomoda ,  Atsushi Takahashi ,  Wakako Ichikawa

IPC分类号： C12P9/00 ,  C12P7/02 ,  C12N9/02 ,  C12N1/21

CPC分类号： C12P7/02 ,  C07C29/145 ,  C12N9/0004 ,  C12N9/0006 ,  C12P7/18 ,  C12P7/26

摘要： It is intended to provide a novel NAD+-independent myo-inositol 2-dehydrogenase which converts myo-inositol into scyllo-inosose in the absence of NAD+; a novel enzyme scyllo-inositol dehydrogenase which stereospecifically reduces scyllo-inosose into scyllo-inositol in the presence of NADH or NADPH; and a novel microorganism which belongs to the genus Acetobacter or Burkholderia and can convert myo-inositol into scyllo-inositol. By using these enzymes or the microorganism, scyllo-inositol is produced. Furthermore, scyllo-inositol is purified by adding boric acid and a metal salt to a liquid mixture containing scyllo-inositol and a neutral saccharide other than scyllo-inositol to form a scyllo-inositol/boric acid complex, separating the complex from the liquid mixture, dissolving the thus separated complex in an acid to give an acidic solution or an acidic suspension and then purifying scyllo-inositol from the acidic solution or the acidic suspension.

摘要翻译： 旨在提供一种新的NAD +独立肌醇2-脱氢酶，其在不存在NAD +的情况下将肌醇转化为鲨肌蛋白; 在NADH或NADPH的存在下，立体选择性地将鲨肌内糖降解成鲨肌醇的新型鲨肌醇肌醇脱氢酶; 和属于醋杆菌属或伯克霍尔德氏菌属的新型微生物，可将肌醇转化为鲨肌醇。 通过使用这些酶或微生物，产生鲨肌醇。 此外，通过向含有鲨肌醇和除了鲨肌醇之外的中性糖的液体混合物中加入硼酸和金属盐以形成鲨肌醇/硼酸络合物，将所述络合物与液体混合物分离，将鲨肌醇纯化 将如此分离的络合物溶解在酸中，得到酸性溶液或酸性悬浮液，然后从酸性溶液或酸性悬浮液中纯化鲨肌醇。

公开(公告)号：US06818430B1

公开(公告)日：2004-11-16

申请号：US09980453

申请日：2002-02-12

申请人： Atsushi Takahashi ,  Kenji Kanbe ,  Tetsuya Mori ,  Yuichi Kita ,  Tsuyoshi Tamamura ,  Tomio Takeuchi

发明人： Atsushi Takahashi ,  Kenji Kanbe ,  Tetsuya Mori ,  Yuichi Kita ,  Tsuyoshi Tamamura ,  Tomio Takeuchi

IPC分类号： C12P1902

CPC分类号： C12R1/18 ,  C12P19/02 ,  C12R1/38 ,  C12R1/64

摘要： Provided are novel processes for the efficient production of L-epi-2-inosose and epi-inositol which are useful either as various medicines or intermediates for the syntheses of various medicines. In the processes, there is used inexpensive myo-inositol as a starting compound. That is, there is now developed a new process which comprises reacting myo-inositol with a gram-negative bacterium capable of converting myo-inositol into L-epi-2-inosose, and thereby producing L-epi-2-inosose by conversion of myo-inositol into L-epi-2-inosose. Further, a novel process is provided, which comprises reacting the so produced L-epi-2-inosose with a reducing agent made of an alkali metal boron hydride or any other alkali metal hydride in an aqueous reaction medium, to produce epi-inositol and myo-inositol, and then isolating epi-inositol from the resulting reaction product composed of the epi-inositol and myo-inositol.

摘要翻译： 提供了用于有效生产L-外 - 二糖和外消旋肌醇的新方法，其可用作各种药物或用于合成各种药物的中间体。 在这些方法中，使用廉价的肌醇作为起始化合物。 也就是说，现在开发了一种新的方法，其包括使肌醇与能够将肌醇转化为L-表 - 2-inosose的革兰氏阴性细菌反应，从而通过转化肌内 - 肌醇进入L-epi-2-inosose。 此外，提供了一种新的方法，其包括使如此制备的L-表 - 2-异糖与在水性反应介质中由碱金属硼氢化物或任何其它碱金属氢化物制成的还原剂反应，以产生表肌醇和 肌醇，然后从由肌醇和肌醇组成的反应产物中分离表肌醇。

公开(公告)号：US07157268B2

公开(公告)日：2007-01-02

申请号：US10852153

申请日：2004-05-25

申请人： Atsushi Takahashi ,  Kenji Kanbe ,  Tetsuya Mori ,  Yuichi Kita ,  Tsuyoshi Tamamura ,  Tomio Takeuchi

发明人： Atsushi Takahashi ,  Kenji Kanbe ,  Tetsuya Mori ,  Yuichi Kita ,  Tsuyoshi Tamamura ,  Tomio Takeuchi

IPC分类号： C12P19/02 ,  C12N1/20

CPC分类号： C12R1/18 ,  C12P19/02 ,  C12R1/38 ,  C12R1/64

摘要： Provided are novel processes for the efficient production of L-epi-2-inosose and epi-inositol which are useful either as various medicines or intermediates for the syntheses of various medicines. In the processes, inexpensive myo-inositol is used as a starting compound which is reacted with a gram-negative bacterium capable of converting myo-inositol into L-epi-2-inosose, and thereby producing L-epi-2-inosose by conversion of myo-inositol into L-epi-2-inosose. A biologically pure culture of Pseudomonas sp. AB 10215 strain is also provided which has a characteristic nature of being capable of converting myo-inositol into L-epi-2-inosose.

摘要翻译： 提供了用于有效生产L-外 - 二糖和外消旋肌醇的新方法，其可用作各种药物或用于合成各种药物的中间体。 在该方法中，使用廉价的肌醇作为起始化合物，其与能够将肌醇转化为L-外 - 二糖的革兰氏阴性细菌反应，从而通过转化产生L-表二-2-糖 的肌醇变为L-epi-2-inosose。 假单胞菌属的生物纯培养物 还提供了AB 10215菌株，其特征在于能够将肌醇转化为L-表 - 2-inosose。

公开(公告)号：US20110059489A1

公开(公告)日：2011-03-10

申请号：US12944592

申请日：2010-11-11

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12P19/26

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB 10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，例如木霉木霉AB 10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624）， 在补充有除甲壳素和几丁质寡糖以外的碳源的培养基和氮源中，在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。

公开(公告)号：US08383368B2

公开(公告)日：2013-02-26

申请号：US12944592

申请日：2010-11-11

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12P19/26 ,  C12N1/14

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB 10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，例如木霉木霉AB 10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624）， 在补充有除甲壳素和几丁质寡糖以外的碳源的培养基和氮源中，在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。

公开(公告)号：US20100055746A1

公开(公告)日：2010-03-04

申请号：US11995104

申请日：2006-07-14

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12P19/02 ,  C12N1/15

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，如木霉木霉AB10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624），在 补充有几丁质和几丁质寡糖以外的碳源的培养基和氮源，以在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。

公开(公告)号：US07998723B2

公开(公告)日：2011-08-16

申请号：US11995104

申请日：2006-07-14

申请人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

发明人： Tetsuya Mori ,  Wakako Ichikawa ,  Yuichi Kita ,  Yasuyuki Tetsuka

IPC分类号： C12N1/14

CPC分类号： C12R1/885 ,  C12P19/26

摘要： N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

摘要翻译： N-乙酰基-D-葡糖胺可以通过培养能够产生N-乙酰基-D-葡糖胺的真菌，如木霉木霉AB10282菌株（FERM BP-10623）或哈茨木霉AB10283菌株（FERM BP-10624），在 补充有几丁质和几丁质寡糖以外的碳源的培养基和氮源，以在培养基中产生并积累N-乙酰基-D-葡糖胺，然后从培养基中收集N-乙酰基-D-葡糖胺。