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1.发明申请METHOD OF DETECTING MILD IMPAIRED GLUCOSE TOLERANCE OR INSULIN SECRETORY DEFECT 审中-公开检测有害葡萄糖耐量或胰岛素分泌缺陷的方法公开(公告)号：US20090042279A1
公开(公告)日：2009-02-12
申请号：US12248876
申请日：2008-10-09
申请人： Masaru YAMAKOSHI , Takuji Kouzuma
发明人： Masaru YAMAKOSHI , Takuji Kouzuma
IPC分类号： C12P1/00
CPC分类号： C12Q1/32 , C12Q1/485 , C12Q1/54 , G01N33/66 , G01N33/6893 , G01N2800/042
摘要： It is intended to provide a noninvasive method of conveniently detecting mild impaired glucose tolerance and/or insulin hyposecretion at the early stage with the use of an enzyme. Namely, mild impaired glucose tolerance and/or insulin hyposecretion at the early stage are detected by quantifying myoinositol secreted into the urine before loading glucose and after loading glucose for a definite period of time with the use of a reagent and comparing the increase (or the increase ratio) in the myoinositol content thus measured with a characteristic level which has been preliminarily determined in normal subjects.
摘要翻译： 旨在提供一种在使用酶的早期方便地检测轻度葡萄糖耐量降低和/或胰岛素分泌不足的非侵入性方法。 即，通过在加载葡萄糖之前和在使用试剂加载葡萄糖一定时间后，通过量化分泌到尿液中的肌醇来检测早期的葡萄糖耐量降低和/或胰岛素分泌不足，并比较增加（或 增加比例）在由正常受试者预先确定的特征水平测量的肌醇含量中。
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2.发明授权Method of detecting mild impaired glucose tolerance or insulin secretory defect 有权检测轻度葡萄糖耐量受损或胰岛素分泌缺陷的方法公开(公告)号：US07452687B2
公开(公告)日：2008-11-18
申请号：US10509120
申请日：2003-03-27
申请人： Masaru Yamakoshi , Takuji Kouzuma
发明人： Masaru Yamakoshi , Takuji Kouzuma
CPC分类号： C12Q1/32 , C12Q1/485 , C12Q1/54 , G01N33/66 , G01N33/6893 , G01N2800/042
摘要： It is intended to provide a noninvasive method of conveniently detecting mild impaired glucose tolerance and/or insulin hyposecretion at the early stage with the use of an enzyme. Namely, mild impaired glucose tolerance and/or hyposecretion at the early stage are detected by quantifying myoinositol secreted into the urine before loading glucose and after loading glucose for a definite period of time with the use of a reagent and comparing the increase (or the increase ratio) in the myoinositol content thus measured with a characteristic level which has been preliminarily determined in normal subjects.
摘要翻译： 旨在提供一种在使用酶的早期方便地检测轻度葡萄糖耐量降低和/或胰岛素分泌不足的非侵入性方法。 即，通过在加载葡萄糖之前和在使用试剂加载葡萄糖一定时间后，将分泌到尿液中的肌醇定量分析来检测早期的葡萄糖耐量降低和/或分泌不足，并比较增加（或增加 比例）在由正常受试者预先确定的特征水平测量的肌醇含量中。
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3.发明申请Method for detecting mild impaired glucose tolerance or insulin hyposecretion 有权检测轻度葡萄糖耐量降低或胰岛素分泌不足的方法公开(公告)号：US20050214885A1
公开(公告)日：2005-09-29
申请号：US10509120
申请日：2003-03-27
申请人： Masaru Yamakoshi , Takuji Kouzuma
发明人： Masaru Yamakoshi , Takuji Kouzuma
CPC分类号： C12Q1/32 , C12Q1/485 , C12Q1/54 , G01N33/66 , G01N33/6893 , G01N2800/042
摘要： It is intended to provide a noninvasive method of conveniently detecting mild impaired glucose tolerance and/or insulin hyposecretion at the early stage with the use of an enzyme. Namely, mild impaired glucose tolerance and/or hyposecretion at the early stage are detected by quantifying myoinositol secreted into the urine before loading glucose and after loading glucose for a definite period of time with the use of a reagent and comparing the increase (or the increase ratio) in the myoinositol content thus measured with a characteristic level which has been preliminarily determined in normal subjects.
摘要翻译： 旨在提供一种在使用酶的早期方便地检测轻度葡萄糖耐量降低和/或胰岛素分泌不足的非侵入性方法。 即，通过在加载葡萄糖之前和在使用试剂加载葡萄糖一定时间后，将分泌到尿液中的肌醇定量分析来检测早期的葡萄糖耐量降低和/或分泌不足，并比较增加（或增加 比例）在由正常受试者预先确定的特征水平测量的肌醇含量中。
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4.发明申请Hemoglobin A1c Determination Method, Enzyme to be Used therefor, and Production Method Thereof 有权血红蛋白A1c测定方法，用于其的酶及其制备方法公开(公告)号：US20080113381A1
公开(公告)日：2008-05-15
申请号：US10557892
申请日：2004-05-21
申请人： Takeshi Matsuoka , Shinji Koga , Takuji Kouzuma , Issei Yoshioka , Atsuhisa Nishimura , Homare Itou , Toshihiko Kumazawa , Takashi Kuroyanagi
发明人： Takeshi Matsuoka , Shinji Koga , Takuji Kouzuma , Issei Yoshioka , Atsuhisa Nishimura , Homare Itou , Toshihiko Kumazawa , Takashi Kuroyanagi
CPC分类号： C12N9/0004 , C12N9/50 , C12Q1/37 , C12R1/01 , C12R1/07 , G01N33/66 , G01N33/723 , G01N2333/95
摘要： There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor.A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method.According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.
摘要翻译： 提供了使用没有分离操作的酶来特异性测定糖化血红蛋白的糖基化β-链N-末端的方法及其测定试剂盒。 从糖基化的β-链N-末端切割糖化氨基酸和/或糖基化肽的蛋白酶，不从糖化血红蛋白或其片段的糖基化的α-链N末端基本上切割糖基化的氨基酸或糖化肽 被筛选 通过使用通过筛选方法获得的蛋白酶来提供糖基化血红蛋白的糖化β-链N-末端的方法和测定试剂盒。 根据本发明，糖化血红蛋白的糖化β-链N-末端可以在不进行分离操作的情况下进行确定。
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公开(公告)号：US07250269B2
公开(公告)日：2007-07-31
申请号：US10470678
申请日：2002-01-30
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）使蛋白酶和至少起作用于糖基化氨基酸的酶的稳定化; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US07943337B2
公开(公告)日：2011-05-17
申请号：US12506695
申请日：2009-07-21
申请人： Takeshi Matsuoka , Shinji Koga , Takuji Kouzuma
发明人： Takeshi Matsuoka , Shinji Koga , Takuji Kouzuma
CPC分类号： C12N9/0004 , C12N9/50 , C12Q1/37 , C12R1/01 , C12R1/07 , G01N33/66 , G01N33/723 , G01N2333/95
摘要： There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.
摘要翻译： 提供了使用不分离操作的酶来特异性测定糖化血红蛋白的糖基化和N-末端的N-末端的方法及其测定试剂盒。 一种蛋白酶，其从糖基化连接的N-末端切割糖基化氨基酸和/或糖化肽，而不从糖化血红蛋白的糖基化α-链N-末端切割糖基化氨基酸或糖化肽 被筛选。 通过使用通过筛选方法获得的蛋白酶提供了特异性测定糖化血红蛋白的糖基化和连结的N-末端的方法和测定试剂盒。 根据本发明，可以在没有分离操作的情况下，具体地确定糖化血红蛋白的糖基化和连结的N-末端。
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公开(公告)号：US20050101771A1
公开(公告)日：2005-05-12
申请号：US10470678
申请日：2002-01-30
IPC分类号： C12Q1/37 , G01N33/68 , G01N33/53 , C07K14/37 , G01N33/569
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）使蛋白酶和至少起作用于糖基化氨基酸的酶的稳定化; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US20110312009A1
公开(公告)日：2011-12-22
申请号：US11831783
申请日：2007-07-31
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) stabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）稳定蛋白酶和至少作用于糖化氨基酸的酶; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US20120129202A1
公开(公告)日：2012-05-24
申请号：US13316404
申请日：2011-12-09
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) stabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）稳定蛋白酶和至少作用于糖化氨基酸的酶; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US08105800B2
公开(公告)日：2012-01-31
申请号：US11831783
申请日：2007-07-31
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Composition for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下方式准确测定糖化蛋白质的组成：1）避免球蛋白和抗坏血酸组分的影响，2）使蛋白酶和至少作用于糖基化氨基酸的酶的稳定化; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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