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公开(公告)号：US07547821B2
公开(公告)日：2009-06-16
申请号：US10869040
申请日：2004-06-17
CPC分类号： C07K14/62 , C12N15/8257
摘要： Commercial production of human insulin can be affected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.
摘要翻译： 人类胰岛素的商业生产可以通过植物种子中的转基因表达来影响。 因此，通过以单链抗体作为融合配偶体的胰岛素的表达，可以重组地实现超过总细胞蛋白质的0.1％的胰岛素积聚的水平。 种子生产提供了作为原料的胰岛素储存和运输的灵活性，胰岛素从储存的种子提取时保留其活性。 此外，由于植物种子的含水量相对较低，所以提取的生物质的量受到限制。
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公开(公告)号：US20050039235A1
公开(公告)日：2005-02-17
申请号：US10869040
申请日：2004-06-17
CPC分类号： C07K14/62 , C12N15/8257
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5′ to 3′ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
摘要翻译： 描述了在植物中产生胰岛素的方法。 在一个实施方案中，本发明提供了一种用于在植物中表达胰岛素的方法，包括：（a）提供嵌合核酸构建体，其以5'至3'的转录方向包含可操作地连接的组分：（i）核酸 能够控制植物种子细胞中表达的序列; 和（ii）编码胰岛素多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 和（c）将植物细胞生长成能种植种子的成熟植物，其中种子表达胰岛素。
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3.发明申请POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS 审中-公开使用多不饱和脂多糖合成酶系统进行异构体生物聚合脂肪酸生产公开(公告)号：US20070245431A1
公开(公告)日：2007-10-18
申请号：US11686850
申请日：2007-03-15
申请人： James Metz , Jerry Kuner , James Lippmeier , Maurice Moloney , Cory Nykiforuk
发明人： James Metz , Jerry Kuner , James Lippmeier , Maurice Moloney , Cory Nykiforuk
CPC分类号： C12N9/1029 , C12N9/93 , C12N15/8247 , C12P7/6427 , C12P7/6472 , Y02P20/52
摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the plant or plant cell, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.
摘要翻译： 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物表达的脂肪酸合成酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加植物或植物细胞中丙二酰辅酶A的水平 ，并且在一个方面，抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。
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