公开(公告)号：US20090280545A1

公开(公告)日：2009-11-12

申请号：US12207761

申请日：2008-09-10

申请人： Michael Mendez ,  Stephen Mayfield ,  Bryan O'Neill ,  Yan Poon ,  Phillip Lee ,  Craig Aaron Behnke ,  Su-Chiung Fang

发明人： Michael Mendez ,  Stephen Mayfield ,  Bryan O'Neill ,  Yan Poon ,  Phillip Lee ,  Craig Aaron Behnke ,  Su-Chiung Fang

IPC分类号： C12P7/04 ,  C12N15/00 ,  C12N1/13 ,  C12P5/02

CPC分类号： C12P23/00 ,  C12N15/902 ,  C12P7/04 ,  C12P9/00

摘要： The present invention provides compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of products. The methods and compositions are particularly useful in the petrochemical industry.

摘要翻译： 本发明提供了通过光合生物产生产物的组合物和方法。 光合生物被遗传修饰以产生产物，分泌物或两者的产物。 所述方法和组合物在石化工业中特别有用。

公开(公告)号：US08318436B2

公开(公告)日：2012-11-27

申请号：US13269414

申请日：2011-10-07

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

IPC分类号： C12Q1/68 ,  C12Q1/34 ,  C12P19/30 ,  C12N15/64 ,  C12N9/42 ,  C12N1/00 ,  C12N1/12 ,  C07H21/02 ,  C07H19/04

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。

公开(公告)号：US20120107853A1

公开(公告)日：2012-05-03

申请号：US13269414

申请日：2011-10-07

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

IPC分类号： C12Q1/34

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。

公开(公告)号：US08143039B2

公开(公告)日：2012-03-27

申请号：US12156450

申请日：2008-05-30

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

IPC分类号： C12P21/00 ,  C12Q1/68 ,  C12N1/13 ,  C12N15/00 ,  C12P7/10 ,  C12P9/00

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

公开(公告)号：US20090253169A1

公开(公告)日：2009-10-08

申请号：US12156450

申请日：2008-05-30

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Yan Poon

IPC分类号： C12P21/00 ,  C12N15/00 ,  C12N1/13 ,  C12P7/10 ,  C12N9/00

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。

公开(公告)号：US20090246766A1

公开(公告)日：2009-10-01

申请号：US12156432

申请日：2008-05-30

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

IPC分类号： C12Q1/68 ,  C12N1/12

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。

公开(公告)号：US08669059B2

公开(公告)日：2014-03-11

申请号：US13585758

申请日：2012-08-14

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。

公开(公告)号：US20120309014A1

公开(公告)日：2012-12-06

申请号：US13585758

申请日：2012-08-14

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。

公开(公告)号：US08268553B2

公开(公告)日：2012-09-18

申请号：US12156432

申请日：2008-05-30

申请人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

发明人： Stephen Mayfield ,  Bryan O'Neill ,  Michael Mendez ,  Kari Mikkelson ,  Yan Poon

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C12N15/8214 ,  Y02E50/16

摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。

公开(公告)号：US20070298050A1

公开(公告)日：2007-12-27

申请号：US11801506

申请日：2007-05-09

申请人： Stephen Mayfield

发明人： Stephen Mayfield

IPC分类号： A61K36/02 ,  A61P31/00 ,  C12N1/12 ,  C12P21/08

CPC分类号： C12N15/79 ,  C12N15/8214 ,  C12N15/8257

摘要： Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of combinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional acitivity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.

摘要翻译： 公开的方法和组合物通过直接替代叶绿体光系统II（PSII）反应中心蛋白编码区来构建包含异源哺乳动物基因的叶绿体，以实现总蛋白超过5％的组合蛋白的表达。 当使用藻类时，藻类表达的蛋白质主要产生为可溶性蛋白质，其中肽的功能活性是完整的。 由于宿主藻类是可食用的，公开了在该生物体中生产用于口服递送的生物制品或蛋白质/肽，特别是肠道活性蛋白质，而不需要纯化。