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    Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
    1.
    发明申请
    Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories 有权

    公开(公告)号：US20060263810A1

    公开(公告)日：2006-11-23

    申请号：US11416501

    申请日：2006-05-02

    申请人： Michel Bergeron ,  Francois Picard ,  Marc Ouellette ,  Paul Roy

    发明人： Michel Bergeron ,  Francois Picard ,  Marc Ouellette ,  Paul Roy

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6895 ,  C12Q1/6844 ,  C12Q1/689 ,  C12Q2600/16

    摘要： DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample DNA from (i) any bacterium, (ii) the species Streptococcus agalactiae, Staphylococcus saprophyticus, Enterococcus faecium, Neisseria meningitidis, Listeria monocytogenes and Candida albicans, and (iii) any species of the genera Streptococcus, Staphylococcus, Enterococcus, Neisseria and Candida are disclosed. DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample antibiotic resistance genes selected from the group consisting of blatem, blarob, blashv, blaoxa, blaZ, aadB, aacC1, aacC2, aacC3, aacA4, aac6′-IIa, ermA, ermB, ermC, mecA, vanA, vanB, vanC, satA, aac(6′)-aph(2″), aad(6), vat, vga, msrA, sul and int are also disclosed. The above microbial species, genera and resistance genes are all clinically relevant and commonly encountered in a variety of clinical specimens. These DNA-based assays are rapid, accurate and can be used in clinical microbiology laboratories for routine diagnosis. These novel diagnostic tools should be useful to improve the speed and accuracy of diagnosis of microbial infections, thereby allowing more effective treatments. Diagnostic kits for (i) the universal detection and quantification of bacteria, and/or (ii) the detection, identification and quantification of the above-mentioned bacterial and fungal species and/or genera, and/or (iii) the detection, identification and quantification of the above-mentioned antibiotic resistance genes are also claimed.

    Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
    2.
    发明申请
    Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories 审中-公开

    公开(公告)号：US20050042606A9

    公开(公告)日：2005-02-24

    申请号：US10121120

    申请日：2002-04-11

    申请人： Michel Bergeron ,  Marc Ouellette ,  Paul Roy

    发明人： Michel Bergeron ,  Marc Ouellette ,  Paul Roy

    IPC分类号： C07K14/195 ,  C07K14/21 ,  C07K14/245 ,  C07K14/26 ,  C07K14/285 ,  C07K14/31 ,  C07K14/315 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07K14/315 ,  C07K14/195 ,  C07K14/21 ,  C07K14/212 ,  C07K14/245 ,  C07K14/26 ,  C07K14/285 ,  C07K14/31 ,  C07K14/3156 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2600/16

    摘要： The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes, Haemophilus influenzae and Moraxella catarrhalis as well as for specific detection of commonly encountered and clinically relevant bacterial antibiotic resistance genes directly from clinical specimens or, alternatively, from a bacterial colony. The above bacterial species can account for as much as 80% of bacterial pathogens isolated in routine microbiology laboratories. The core of this invention consists primarily of the DNA sequences from all species-specific genomic DNA fragments selected by hybridization from genomic libraries or, alternatively, selected from data banks as well as any oligonucleotide sequences derived from these sequences which can be used as probes or amplification primers for PCR or any other nucleic acid amplification methods. This invention also includes DNA sequences from the selected clinically relevant antibiotic resistance genes. With these methods, bacteria can be detected (universal primers and/or probes) and identified (species-specific primers and/or probes) directly from the clinical specimens or from an isolated bacterial colony. Bacteria are further evaluated for their putative susceptibility to antibiotics by resistance gene detection (antibiotic resistance gene specific primers and/or probes). Diagnostic kits for the detection of the presence, for the bacterial identification of the above-mentioned bacterial species and for the detection of antibiotic resistance genes are also claimed. These kits for the rapid (one hour or less) and accurate diagnosis of bacterial infections and antibiotic resistance will gradually replace conventional methods currently used in clinical microbiology laboratories for routine diagnosis. They should provide tools to clinicians to help prescribe promptly optimal treatments when necessary. Consequently, these tests should contribute to saving human lives, rationalizing treatment, reducing the development of antibiotic resistance and avoid unnecessary hospitalizations.

    Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
    3.
    发明申请
    Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories 审中-公开

    公开(公告)号：US20070009947A1

    公开(公告)日：2007-01-11

    申请号：US11503368

    申请日：2006-08-10

    申请人： Michel Bergeron ,  Marc Ouellette ,  Paul Roy

    发明人： Michel Bergeron ,  Marc Ouellette ,  Paul Roy

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C07K14/315 ,  C07K14/195 ,  C07K14/21 ,  C07K14/212 ,  C07K14/245 ,  C07K14/26 ,  C07K14/285 ,  C07K14/31 ,  C07K14/3156 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2600/16

    摘要： The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes, Haemophilus influenzae and Moraxella catarrhalis as well as for specific detection of commonly encountered and clinically relevant bacterial antibiotic resistance genes directly from clinical specimens or, alternatively, from a bacterial colony. The above bacterial species can account for as much as 80% of bacterial pathogens isolated in routine microbiology laboratories. The core of this invention consists primarily of the DNA sequences from all species-specific genomic DNA fragments selected by hybridization from genomic libraries or, alternatively, selected from data banks as well as any oligonucleotide sequences derived from these sequences which can be used as probes or amplification primers for PCR or any other nucleic acid amplification methods. This invention also includes DNA sequences from the selected clinically relevant antibiotic resistance genes. With these methods, bacteria can be detected (universal primers and/or probes) and identified (species-specific primers and/or probes) directly from the clinical specimens or from an isolated bacterial colony. Bacteria are further evaluated for their putative susceptibility to antibiotics by resistance gene detection (antibiotic resistance gene specific primers and/or probes). Diagnostic kits for the detection of the presence, for the bacterial identification of the above-mentioned bacterial species and for the detection of antibiotic resistance genes are also claimed. These kits for the rapid (one hour or less) and accurate diagnosis of bacterial infections and antibiotic resistance will gradually replace conventional methods currently used in clinical microbiology laboratories for routine diagnosis. They should provide tools to clinicians to help prescribe promptly optimal treatments when necessary. Consequently, these tests should contribute to saving human lives, rationalizing treatment, reducing the development of antibiotic resistance and avoid unnecessary hospitalizations.

    Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
    4.
    发明申请
    Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories 有权

    公开(公告)号：US20070105129A1

    公开(公告)日：2007-05-10

    申请号：US11416499

    申请日：2006-05-01

    申请人： Michel Bergeron ,  Marc Ouellette ,  Paul Roy

    发明人： Michel Bergeron ,  Marc Ouellette ,  Paul Roy

    IPC分类号： C12Q1/68

    CPC分类号： C07K14/315 ,  C07K14/195 ,  C07K14/21 ,  C07K14/212 ,  C07K14/245 ,  C07K14/26 ,  C07K14/285 ,  C07K14/31 ,  C07K14/3156 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2600/16

    摘要： The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes, Haemophilus influenzae and Moraxella catarrhalis as well as for specific detection of commonly encountered and clinically relevant bacterial antibiotic resistance genes directly from clinical specimens or, alternatively, from a bacterial colony. The above bacterial species can account for as much as 80% of bacterial pathogens isolated in routine microbiology laboratories. The core of this invention consists primarily of the DNA sequences from all species-specific genomic DNA fragments selected by hybridization from genomic libraries or, alternatively, selected from data banks as well as any oligonucleotide sequences derived from these sequences which can be used as probes or amplification primers for PCR or any other nucleic acid amplification methods. This invention also includes DNA sequences from the selected clinically relevant antibiotic resistance genes. With these methods, bacteria can be detected (universal primers and/or probes) and identified (species-specific primers and/or probes) directly from the clinical specimens or from an isolated bacterial colony. Bacteria are further evaluated for their putative susceptibility to antibiotics by resistance gene detection (antibiotic resistance gene specific primers and/or probes). Diagnostic kits for the detection of the presence, for the bacterial identification of the above-mentioned bacterial species and for the detection of antibiotic resistance genes are also claimed. These kits for the rapid (one hour or less) and accurate diagnosis of bacterial infections and antibiotic resistance will gradually replace conventional methods currently used in clinical microbiology laboratories for routine diagnosis. They should provide tools to clinicians to help prescribe promptly optimal treatments when necessary. Consequently, these tests should contribute to saving human lives, rationalizing treatment, reducing the development of antibiotic resistance and avoid unnecessary hospitalizations.

    Reducing ad impact to browser onload event
    6.
    发明授权
    Reducing ad impact to browser onload event 有权
    减少对浏览器onload事件的广告影响

    公开(公告)号：US09177076B2

    公开(公告)日：2015-11-03

    申请号：US13343746

    申请日：2012-01-05

    申请人： Dinko Bajric ,  Gregory Bershansky ,  Alex Polak ,  Paul Roy

    发明人： Dinko Bajric ,  Gregory Bershansky ,  Alex Polak ,  Paul Roy

    IPC分类号： G06F17/20 ,  G06F17/30 ,  G06F17/22

    CPC分类号： G06F17/30902 ,  G06F17/2247

    摘要： Various embodiments pertain to techniques for decreasing an amount of time to a browser onload event by asynchronously loading web page content. In various embodiments, frames are utilized to trigger an onload event before all content in a web page is loaded. In some embodiments, various components of the web page, such as advertisements, are provided in frames, and the loading of the components is triggered by an onload event of the frame. In other words, an empty frame can be provided for the purposes of triggering both a frame onload event and an onload event for the web page. The frame onload event can cause a page script to be executed effective to load the components into the frames. In some embodiments, two or more nested frames are utilized to provide asynchronous loading of web page components while maintaining compatibility of the advertisement or multimedia items and the web page.

    摘要翻译： 各种实施例涉及通过异步加载网页内容来减少浏览器上载事件的时间量的技术。 在各种实施例中，在加载网页中的所有内容之前，利用帧来触发上载事件。 在一些实施例中，诸如广告的网页的各种组件以帧提供，并且组件的加载由帧的加载事件触发。 换句话说，可以提供一个空框架，用于触发网页的onload事件和onload事件。 frame onload事件可以使得页面脚本被执行有效地将组件加载到帧中。 在一些实施例中，利用两个或多个嵌套帧来提供网页组件的异步加载，同时保持广告或多媒体项目与网页的兼容性。

    Reducing Ad Impact to Browser Onload Event
    7.
    发明申请
    Reducing Ad Impact to Browser Onload Event 有权
    减少对浏览器上载事件的广告影响

    公开(公告)号：US20130179767A1

    公开(公告)日：2013-07-11

    申请号：US13343746

    申请日：2012-01-05

    申请人： Dinko Bajric ,  Gregory Bershansky ,  Alex Polak ,  Paul Roy

    发明人： Dinko Bajric ,  Gregory Bershansky ,  Alex Polak ,  Paul Roy

    IPC分类号： G06F17/00

    CPC分类号： G06F17/30902 ,  G06F17/2247

    摘要： Various embodiments pertain to techniques for decreasing an amount of time to a browser onload event by asynchronously loading web page content. In various embodiments, frames are utilized to trigger an onload event before all content in a web page is loaded. In some embodiments, various components of the web page, such as advertisements, are provided in frames, and the loading of the components is triggered by an onload event of the frame. In other words, an empty frame can be provided for the purposes of triggering both a frame onload event and an onload event for the web page. The frame onload event can cause a page script to be executed effective to load the components into the frames. In some embodiments, two or more nested frames are utilized to provide asynchronous loading of web page components while maintaining compatibility of the advertisement or multimedia items and the web page.

    摘要翻译： 各种实施例涉及通过异步加载网页内容来减少浏览器上载事件的时间量的技术。 在各种实施例中，在加载网页中的所有内容之前，利用帧来触发上载事件。 在一些实施例中，诸如广告的网页的各种组件以帧提供，并且组件的加载由帧的加载事件触发。 换句话说，可以提供一个空框架，用于触发网页的onload事件和onload事件。 frame onload事件可以使得页面脚本被执行有效地将组件加载到帧中。 在一些实施例中，利用两个或多个嵌套帧来提供网页组件的异步加载，同时保持广告或多媒体项目与网页的兼容性。

    Network access control based on program state
    8.
    发明申请
    Network access control based on program state 有权
    基于程序状态的网络访问控制

    公开(公告)号：US20090064306A1

    公开(公告)日：2009-03-05

    申请号：US11895755

    申请日：2007-08-27

    申请人： Paul Roy ,  Khalid Al-Azzawe ,  Cale Carter ,  Ambarish Chitnis ,  Richard Masao Hawes

    发明人： Paul Roy ,  Khalid Al-Azzawe ,  Cale Carter ,  Ambarish Chitnis ,  Richard Masao Hawes

    IPC分类号： G06F13/14

    CPC分类号： H04L63/102

    摘要： A gateway controls access to a region of a network by either granting or denying a client machine access to the network region based on whether a particular program is running on the client machine. A program is installed on the client machine which sends a detectable indication that the program is running. When it is detected that the program is running, the gateway allows the client machine access to the network region. When the program is not detected to be running, the gateway denies the client machine access to the network region.

    摘要翻译： 网关通过根据特定程序是否在客户端计算机上运行来授予或拒绝客户机访问网络区域来控制对网络区域的访问。 一个程序安装在客户机上，发送程序运行的可检测指示。 当检测到程序正在运行时，网关允许客户机访问网络区域。 当程序未检测到正在运行时，网关将拒绝客户机访问网络区域。