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1.发明申请Highly conserved genes and their use to generate probes and primers for detection of microorganisms 审中-公开高度保守的基因及其用于产生用于检测微生物的探针和引物公开(公告)号：US20100267012A1
公开(公告)日：2010-10-21
申请号：US11522253
申请日：2006-09-14
申请人： Michel G. Bergeron , Maurice Boissinot , Ann Huletsky , Christian Menard , Marc Ouellette , Francois Picard , Paul H. Roy
发明人： Michel G. Bergeron , Maurice Boissinot , Ann Huletsky , Christian Menard , Marc Ouellette , Francois Picard , Paul H. Roy
CPC分类号： C12Q1/689 , C12Q1/14 , C12Q2600/156 , C12Q2600/16 , C12Q2600/166 , G01N2333/195 , G01N2333/31
摘要： Compositions and methods for the detection of vancomycin-resistant pathogens using primers and/or probes to the vanA and vanB genes.
摘要翻译： 使用引物和/或探针对vanA和vanB基因检测万古霉素抗性病原体的组合物和方法。
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2.发明申请Method for the preparation of reagents for amplification and/or detection of nucleic acids that exhibit no significant contamination by nucleic acids 审中-公开用于制备用于扩增和/或检测不显示核酸显着污染的核酸的试剂的方法公开(公告)号：US20050037349A1
公开(公告)日：2005-02-17
申请号：US10469419
申请日：2003-04-11
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q2527/125 , C12Q2523/313 , C12Q2531/113
摘要： The present invention decribes reagents free of detectable contaminating nucleic acids for performing highly sensitive and specific nucleic acids amplification and/or detection. It relates to an improvement in the technology of nucleic acid inactivation prior to nucleic acid testing (NAT) in order to prevent false-positive results. Specifically, this invention describes optimized and standardized reagents and ultra-violet treatment to achieve an effective and highly reproducible nucleic acid inactivation prior to NAT without substantially affecting the performance of the assay. More specifically, this nucleic acid inactivation process resulted in a reduction of up to four logs of the background signal associated with the PCR (polymerase chain reaction) amplification of DNA contaminating PCR reagents. This optimized and standardized method is also adaptable for use with NAT technologies other than PCR.
摘要翻译： 本发明描述了不含可检测的污染性核酸的试剂，用于进行高灵敏度和特异性核酸扩增和/或检测。 它涉及核酸测试（NAT）之前的核酸灭活技术的改进，以防止假阳性结果。 具体地，本发明描述了优化的和标准化的试剂和紫外线处理，以在NAT之前实现有效且高度可重现的核酸灭活，而基本上不影响测定的性能。 更具体地，该核酸失活过程导致与DNA污染性PCR试剂的PCR（聚合酶链式反应）扩增相关的背景信号降低多达四个对数。 这种优化和标准化的方法也适用于除PCR以外的NAT技术。
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3.发明授权公开(公告)号：US08183015B2
公开(公告)日：2012-05-22
申请号：US12773581
申请日：2010-05-04
IPC分类号： C12P19/34
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6848 , C12Q1/6851 , C12Q1/689 , C12Q2600/16 , C12Q2600/166 , C12Q2545/101
摘要： This invention relates to reagent comprising: any one of cells, viral particles, organelles, parasites, cells comprising organelles, cells comprising viral particles, cells comprising parasites, cells comprising bacterial cells and any combination thereof, the cells, viral particles, organelles or parasites comprising at least one nucleic acid sequence serving as an internal control (IC) target for nucleic acid testing (NAT) assay; wherein the reagent is suitable to be added to a test sample undergoing sample preparation to release, concentrate and/or purify nucleic acids and amplification and/or detection of nucleic acids so as to be used to verify: (i) the efficiency of sample preparation; and (ii) the efficiency of nucleic acid amplification and/or detection. The present invention also relates to a method to verify or validate the preparation and amplification and/or detection of a nucleic acid target sequence in a sample spiked with a reagent of the present invention.
摘要翻译： 本发明涉及试剂，其包含：细胞，病毒颗粒，细胞器，寄生虫，包含细胞器的细胞，包含病毒颗粒的细胞，包含寄生虫的细胞，包含细菌细胞的细胞及其任何组合，细胞，病毒颗粒，细胞器或寄生虫 包括用作核酸测试（NAT）测定的内部对照（IC）靶的至少一个核酸序列; 其中所述试剂适于加入经历样品制备的测试样品以释放，浓缩和/或纯化核酸，并扩增和/或检测核酸，以便用于验证：（i）样品制备的效率 ; 和（ii）核酸扩增和/或检测的效率。 本发明还涉及验证或验证掺有本发明试剂的样品中核酸靶序列的制备和扩增和/或检测的方法。
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4.发明申请公开(公告)号：US20100233720A1
公开(公告)日：2010-09-16
申请号：US12773581
申请日：2010-05-04
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6848 , C12Q1/6851 , C12Q1/689 , C12Q2600/16 , C12Q2600/166 , C12Q2545/101
摘要： This invention relates to reagent comprising: any one of cells, viral particles, organelles, parasites, cells comprising organelles, cells comprising viral particles, cells comprising parasites, cells comprising bacterial cells and any combination thereof, the cells, viral particles, organelles or parasites comprising at least one nucleic acid sequence serving as an internal control (IC) target for nucleic acid testing (NAT) assay; wherein the reagent is suitable to be added to a test sample undergoing sample preparation to release, concentrate and/or purify nucleic acids and amplification and/or detection of nucleic acids so as to be used to verify: (i) the efficiency of sample preparation; and (ii) the efficiency of nucleic acid amplification and/or detection. The present invention also relates to a method to verify or validate the preparation and amplification and/or detection of a nucleic acid target sequence in a sample spiked with a reagent of the present invention.
摘要翻译： 本发明涉及试剂，其包含：细胞，病毒颗粒，细胞器，寄生虫，包含细胞器的细胞，包含病毒颗粒的细胞，包含寄生虫的细胞，包含细菌细胞的细胞及其任何组合，细胞，病毒颗粒，细胞器或寄生虫 包括用作核酸测试（NAT）测定的内部对照（IC）靶的至少一个核酸序列; 其中所述试剂适于加入经历样品制备的测试样品以释放，浓缩和/或纯化核酸，并扩增和/或检测核酸，以便用于验证：（i）样品制备的效率 ; 和（ii）核酸扩增和/或检测的效率。 本发明还涉及验证或验证掺有本发明试剂的样品中核酸靶序列的制备和扩增和/或检测的方法。
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5.发明授权公开(公告)号：US07718402B2
公开(公告)日：2010-05-18
申请号：US10538442
申请日：2003-12-15
IPC分类号： C12P19/34
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6848 , C12Q1/6851 , C12Q1/689 , C12Q2600/16 , C12Q2600/166 , C12Q2545/101
摘要： This invention relates to reagent comprising: any one of cells, viral particles, organelles, parasites, cells comprising organelles, cells comprising viral particles, cells comprising parasites, cells comprising bacterial cells and any combination thereof, the cells, viral particles, organelles or parasites comprising at least one nucleic acid sequence serving as an internal control (IC) target for nucleic acid testing (NAT) assay; wherein the reagent is suitable to be added to a test sample undergoing sample preparation to release, concentrate and/or purify nucleic acids and amplification and/or detection of nucleic acids so as to be used to verify: (i) the efficiency of sample preparation; and (ii) the efficiency of nucleic acid amplification and/or detection. The present invention also relates to a method to verify or validate the preparation and amplification and/or detection of a nucleic acid target sequence in a sample spiked with a reagent of the present invention.
摘要翻译： 本发明涉及试剂，其包含：细胞，病毒颗粒，细胞器，寄生虫，包含细胞器的细胞，包含病毒颗粒的细胞，包含寄生虫的细胞，包含细菌细胞的细胞及其任何组合，细胞，病毒颗粒，细胞器或寄生虫 包括用作核酸测试（NAT）测定的内部对照（IC）靶的至少一个核酸序列; 其中所述试剂适于加入经历样品制备的测试样品以释放，浓缩和/或纯化核酸，并扩增和/或检测核酸，以便用于验证：（i）样品制备的效率 ; 和（ii）核酸扩增和/或检测的效率。 本发明还涉及验证或验证掺有本发明试剂的样品中核酸靶序列的制备和扩增和/或检测的方法。
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6.发明申请公开(公告)号：US20070015139A1
公开(公告)日：2007-01-18
申请号：US10538442
申请日：2003-12-15
CPC分类号： C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6848 , C12Q1/6851 , C12Q1/689 , C12Q2600/16 , C12Q2600/166 , C12Q2545/101
摘要： This invention relates to reagent comprising: any one of cells, viral particles, organelles, parasites, cells comprising organelles, cells comprising viral particles, cells comprising parasites, cells comprising bacterial cells and any combination thereof, the cells, viral particles, organelles or parasites comprising at least one nucleic acid sequence serving as an internal control (IC) target for nucleic acid testing (NAT) assay; wherein the reagent is suitable to be added to a test sample undergoing sample preparation to release, concentrate and/or purify nucleic acids and amplification and/or detection of nucleic acids so as to be used to verify: (i) the efficiency of sample preparation; and (ii) the efficiency of nucleic acid amplification and/or detection. The present invention also relates to a method to verify or validate the preparation and amplification and/or detection of a nucleic acid target sequence in a sample spiked with a reagent of the present invention.
摘要翻译： 本发明涉及试剂，其包含：细胞，病毒颗粒，细胞器，寄生虫，包含细胞器的细胞，包含病毒颗粒的细胞，包含寄生虫的细胞，包含细菌细胞的细胞及其任何组合，细胞，病毒颗粒，细胞器或寄生虫 包括用作核酸测试（NAT）测定的内部对照（IC）靶的至少一个核酸序列; 其中所述试剂适于加入经历样品制备的测试样品以释放，浓缩和/或纯化核酸，并扩增和/或检测核酸，以便用于验证：（i）样品制备的效率 ; 和（ii）核酸扩增和/或检测的效率。 本发明还涉及验证或验证掺有本发明试剂的样品中核酸靶序列的制备和扩增和/或检测的方法。
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公开(公告)号：US20060263810A1
公开(公告)日：2006-11-23
申请号：US11416501
申请日：2006-05-02
申请人： Michel Bergeron , Francois Picard , Marc Ouellette , Paul Roy
发明人： Michel Bergeron , Francois Picard , Marc Ouellette , Paul Roy
CPC分类号： C12Q1/6895 , C12Q1/6844 , C12Q1/689 , C12Q2600/16
摘要： DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample DNA from (i) any bacterium, (ii) the species Streptococcus agalactiae, Staphylococcus saprophyticus, Enterococcus faecium, Neisseria meningitidis, Listeria monocytogenes and Candida albicans, and (iii) any species of the genera Streptococcus, Staphylococcus, Enterococcus, Neisseria and Candida are disclosed. DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample antibiotic resistance genes selected from the group consisting of blatem, blarob, blashv, blaoxa, blaZ, aadB, aacC1, aacC2, aacC3, aacA4, aac6′-IIa, ermA, ermB, ermC, mecA, vanA, vanB, vanC, satA, aac(6′)-aph(2″), aad(6), vat, vga, msrA, sul and int are also disclosed. The above microbial species, genera and resistance genes are all clinically relevant and commonly encountered in a variety of clinical specimens. These DNA-based assays are rapid, accurate and can be used in clinical microbiology laboratories for routine diagnosis. These novel diagnostic tools should be useful to improve the speed and accuracy of diagnosis of microbial infections, thereby allowing more effective treatments. Diagnostic kits for (i) the universal detection and quantification of bacteria, and/or (ii) the detection, identification and quantification of the above-mentioned bacterial and fungal species and/or genera, and/or (iii) the detection, identification and quantification of the above-mentioned antibiotic resistance genes are also claimed.
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8.发明授权Method and device for emulating a printer for a personal computer using a facimile-machine printer 失效用于仿真用于个人计算机的打印机的方法和装置，所述打印机使用便携机打印机
公开(公告)号：US5680227A
公开(公告)日：1997-10-21
申请号：US305089
申请日：1994-09-13
申请人： Francois Picard
发明人： Francois Picard
CPC分类号： H04N1/00238 , G06K15/00 , H04N1/00236
摘要： A device for emulating a personal computer printer with a facsimile-machine printer (14) including a buffer memory (21) which receives data from the personal computer (3), circuits (22, 24) coupled to both a command buffer circuit (23) and a calculation block (25). The circuits (22, 24), command buffer circuit (23) and the calculation block (25) control the facsimile-machine printer (14) in response to the data, determine the availability the facsimile-machine printer (14) by effecting cyclic scanning of the facsimile-machine and control the printing of the facsimile-machine (14) printer in response to the data when the facsimile-machine printer (14) is determined to be available for printing.
摘要翻译： 一种用于使用传真机打印机（14）模拟个人计算机打印机的装置，包括从个人计算机（3）接收数据的缓冲存储器（21），耦合到命令缓冲器电路（23）的电路（22,24） ）和计算块（25）。 电路（22,24），指令缓冲电路（23）和计算块（25）响应于数据控制传真机打印机（14），通过执行传真机打印机（14）的循环 当确定传真机打印机（14）可用于打印时，响应于数据，扫描传真机并控制传真机（14）打印机的打印。
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