公开(公告)号：US06759224B2

公开(公告)日：2004-07-06

申请号：US09919854

申请日：2001-08-02

申请人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

发明人： Mike Farwick ,  Klaus Huthmacher ,  Jennifer Brehme ,  Walter Pfefferle ,  Michael Binder ,  Dieter Greissinger ,  Georg Thierbach

IPC分类号： C12N914

CPC分类号： C12N9/88 ,  A23K10/12 ,  A23K20/142 ,  A23K40/35 ,  A23K50/10 ,  C12P13/08 ,  C12P13/12

摘要： An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.

摘要翻译： 一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码 多肽，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含至少15个连续的多核苷酸的多核苷酸 a），b）或c）的多核苷酸序列的核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少sahH基因以增强形式存在，以及使用多核苷酸 含有根据本发明的序列作为杂交探针。

公开(公告)号：US06921651B2

公开(公告)日：2005-07-26

申请号：US10098626

申请日：2002-03-18

申请人： Mike Farwick ,  Brigitte Bathe ,  Jennifer Brehme ,  Klaus Huthmacher

发明人： Mike Farwick ,  Brigitte Bathe ,  Jennifer Brehme ,  Klaus Huthmacher

IPC分类号： C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12N15/55 ,  C12P13/04 ,  C12P13/08

CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12P13/08

摘要： The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofructokinase are/is attenuated, enriching the desired L-amino acid in the medium or in the cells of the bacteria, and isolating the L-amino acid. Optionally bacteria are employed in which, in addition, further genes of the biosynthetic pathway of the desired L-amino acid are enhanced, or bacteria are employed in which the metabolic pathways that diminish the formation of the desired L-amino acid are at least partly switched off.

摘要翻译： 本发明涉及一种制备L-氨基酸的方法。 该方法包括发酵产生所需L-氨基酸的棒状细菌，其中至少编码6-磷酸果糖激酶的基因和/或编码1-磷酸果糖激酶的基因被减毒，从而富集所需的L-氨基酸 培养基或细胞中的细胞，并分离L-氨基酸。 任选地，使用细菌，其中另外还需要增加所需L-氨基酸的生物合成途径的其它基因，或使用细菌，其中减少所需L-氨基酸形成的代谢途径至少部分地 关闭