公开(公告)号：US20050249755A1

公开(公告)日：2005-11-10

申请号：US11071651

申请日：2005-03-03

申请人： Mikeljon Nikolich ,  David Hoover ,  R. Roop ,  Gregory Robertson

发明人： Mikeljon Nikolich ,  David Hoover ,  R. Roop ,  Gregory Robertson

IPC分类号： A61K39/10 ,  C12N1/21 ,  C12N1/36 ,  C12N15/52 ,  C12N15/74

CPC分类号： C12N15/52 ,  A61K39/098 ,  A61K2039/522 ,  C12N1/36

摘要： Live attenuated vaccines against brucellosis are described. New mutant strains of Brucella melitensis have been developed, which are attenuated via deletion of the hfq and/or purEK sites. The purEK deletion site does not include insertion of a kanamycin resistance determinant marker or any other introduced antibiotic resistance marker. The hfq deletion site preferably does not include insertion of a kanamycin resistance determinant marker or any other introduced antibiotic resistance marker.

摘要翻译： 描述针对布鲁氏菌病的减毒活疫苗。 已经开发了新的Brucella melitensis突变菌株，其通过缺失hfq和/或purEK位点而减毒。 purEK缺失位点不包括卡那霉素抗性决定簇标记物或任何其他引入的抗生素抗性标记物的插入。 hfq缺失位点优选不包括卡那霉素抗性决定簇标记物或任何其它引入的抗生素抗性标记物的插入。