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公开(公告)号：US20230099076A1
公开(公告)日：2023-03-30
申请号：US17955430
申请日：2022-09-28
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
IPC: A01H4/00
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US20220010320A1
公开(公告)日：2022-01-13
申请号：US17370598
申请日：2021-07-08
Applicant: Monsanto Technology LLC
Inventor： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
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公开(公告)号：US20210171964A1
公开(公告)日：2021-06-10
申请号：US17127357
申请日：2020-12-18
Applicant: Monsanto Technology LLC
Inventor： Jyoti R. Rout
Abstract: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
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公开(公告)号：US20190082630A1
公开(公告)日：2019-03-21
申请号：US16106342
申请日：2018-08-21
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
IPC: A01H4/00
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US11091767B2
公开(公告)日：2021-08-17
申请号：US16516025
申请日：2019-07-18
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Whitney R. Adams, Jr. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells, such as corn plant immature embryo for bacterial-mediated transformation comprising contacting the plant cells, such as corn plant immature embryo with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells, such as corn plant immature embryo for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells, such as corn plant immature embryo in various storage conditions, thus improving their competency for transformation and tissue culture.
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Patent Agency Ranking
公开(公告)号：US11091766B2
公开(公告)日：2021-08-17
申请号：US15977265
申请日：2018-05-11
Applicant: Monsanto Technology LLC
Inventor： Anisha Akula , David R. Duncan , Brenda A. Lowe , Michael T. Mann , William L. Petersen , Jyoti R. Rout , David D. Songstad , Joel B. Wilks , Wanggen Zhang
Abstract: Production of transformed plants is achieved by placing Agrobacterium-inoculated explants directly onto a medium containing selective agents for suppressing growth of Agrobacterium and killing non-transformed explant cells.
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公开(公告)号：US20200010838A1
公开(公告)日：2020-01-09
申请号：US16516025
申请日：2019-07-18
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Whitney R. Adams, JR. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
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公开(公告)号：US20150013034A1
公开(公告)日：2015-01-08
申请号：US14483748
申请日：2014-09-11
Applicant: Monsanto Technology LLC
Inventor： Jyoti R. Rout , Brenda Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
IPC: C12N15/82
CPC classification number: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
Abstract translation: 本发明提供了通过简单且快速的方案转化单子叶植物的方法，以获得能够在4-8周内种植到土壤中的再生植物。 还提供了相关的细胞培养基和生长条件，以及通过该方法获得的植物和植物部分。 此外，还提供了通过本发明的方法筛选用于可变形性的顽固植物基因型的方法。 此外，还提供了通过本发明的方法扩展用于生产转基因植物的优先发展窗口的系统。
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公开(公告)号：US20140051078A1
公开(公告)日：2014-02-20
申请号：US13973243
申请日：2013-08-22
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Jyoti R. Rout
IPC: C12N15/82
CPC classification number: C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
Abstract: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
Abstract translation: 本发明提供用于鉴定再生转化植物和分化转化植物部分的方法，所述转化植物和分化的转化植物部分在再生细胞以获得分化组织之前不经植物细胞获得选择性条件 在具体实施方案中，植物细胞是玉米植物细胞。 还提供了用于种植和处理植物的方法，包括鉴定显示特定感兴趣特征的植物。
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公开(公告)号：US20210332375A1
公开(公告)日：2021-10-28
申请号：US17317255
申请日：2021-05-11
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, JR. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
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