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公开(公告)号：US10433503B2
公开(公告)日：2019-10-08
申请号：US15452519
申请日：2017-03-07
发明人： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
摘要： The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US20150007370A1
公开(公告)日：2015-01-01
申请号：US14314963
申请日：2014-06-25
发明人： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
IPC分类号： A01H5/00
CPC分类号： A01H5/00 , A01G7/00 , A01H4/001 , A01H4/003 , A01H4/008 , A01H5/10 , C12N15/82 , C12N15/8201 , C12N15/8205
摘要： The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
摘要翻译： 本发明公开并要求用于快速机械分离适于转化或组织培养的单子叶植物组织的方法和装置。 本发明包括用于基本上分离靶植物组织以用作可转化外植体的机械装置，以及转基因植物和植物组织的繁殖。
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公开(公告)号：US20200053970A1
公开(公告)日：2020-02-20
申请号：US16553911
申请日：2019-08-28
发明人： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
摘要： The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US20180119157A1
公开(公告)日：2018-05-03
申请号：US15720759
申请日：2017-09-29
发明人： Jyoti R. Rout , Brenda Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8209 , C12N15/821 , C12N15/8212 , C12N15/8265
摘要： The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
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公开(公告)号：US20170238489A1
公开(公告)日：2017-08-24
申请号：US15452519
申请日：2017-03-07
发明人： Whitney R. Adams, JR. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
CPC分类号： A01H4/003 , A01G7/00 , A01H4/001 , A01H4/008 , A01H5/10 , C12N15/82 , C12N15/8201 , C12N15/8205
摘要： The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US09648814B2
公开(公告)日：2017-05-16
申请号：US14314963
申请日：2014-06-25
发明人： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
CPC分类号： A01H5/00 , A01G7/00 , A01H4/001 , A01H4/003 , A01H4/008 , A01H5/10 , C12N15/82 , C12N15/8201 , C12N15/8205
摘要： The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US20130239253A1
公开(公告)日：2013-09-12
申请号：US13791186
申请日：2013-03-08
发明人： Jyoti R. Rout , Brenda A. Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
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公开(公告)号：US11212972B2
公开(公告)日：2022-01-04
申请号：US16553911
申请日：2019-08-28
发明人： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brenda A. Lowe , Michael Spencer , Michael T. Mann
摘要： The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.
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公开(公告)号：US09783813B2
公开(公告)日：2017-10-10
申请号：US14483748
申请日：2014-09-11
发明人： Jyoti R. Rout , Brenda Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
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公开(公告)号：US08853488B2
公开(公告)日：2014-10-07
申请号：US13791186
申请日：2013-03-08
发明人： Jyoti R. Rout , Brenda A. Lowe , John Purcell , Anne Spelletich , Michael Spencer , Melissa Way
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8202 , C12N15/8206 , C12N15/8207 , C12N15/8209 , C12N15/821 , C12N15/8265 , C12Q1/6876 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Q1/6827 , C12Q1/686
摘要： The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
摘要翻译： 本发明提供了通过简单且快速的方案转化单子叶植物的方法，以获得能够在4-8周内种植到土壤中的再生植物。 还提供了相关的细胞培养基和生长条件，以及通过该方法获得的植物和植物部分。 此外，还提供了通过本发明的方法筛选用于可变形性的顽固植物基因型的方法。 此外，还提供了通过本发明的方法扩展用于生产转基因植物的优先发展窗口的系统。
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