公开(公告)号：US07005048B1

公开(公告)日：2006-02-28

申请号：US09807692

申请日：2000-10-02

申请人： Motokazu Watanabe ,  Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Nakayama ,  Shoji Miyazaki ,  Hideyuki Baba

发明人： Motokazu Watanabe ,  Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Nakayama ,  Shoji Miyazaki ,  Hideyuki Baba

IPC分类号： G01N27/327

CPC分类号： C12Q1/006

摘要： The present invention provides a high-performance glucose sensor having excellent storage stability and an improved response characteristic. This sensor comprises: an electrically insulating base plate; an electrode system including at least a working electrode and a counter electrode formed on the base plate; and a reaction layer containing at least pyrrolo-quinoline quinone dependent glucose dehydrogenase, formed in contact with or in the vicinity of the electrode system, and the reaction layer contains at least one kind of additive selected from the group consisting of gluconic acid and salts thereof.

摘要翻译： 本发明提供一种具有优异的储存稳定性和改善的响应特性的高性能葡萄糖传感器。 该传感器包括：电绝缘基板; 电极系统，其至少包括形成在所述基板上的工作电极和对电极; 和至少含有与电极体系接触或附近形成的吡咯并喹啉醌依赖型葡萄糖脱氢酶的反应层，反应层含有选自葡萄糖酸及其盐的至少一种添加剂 。

公开(公告)号：US06773564B1

公开(公告)日：2004-08-10

申请号：US09406832

申请日：1999-09-28

申请人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

发明人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai ,  Junko Iwata ,  Shoji Miyazaki ,  Hideyuki Baba ,  Seiji Takeshima

IPC分类号： G01N27327

CPC分类号： C12Q1/006

摘要： The present invention provides a high performance glucose sensor which can demonstrate high stability against preservation and produces only a low blank value. The glucose sensor comprises an electrically insulating base plate, an electrode system formed on the base plate, and a reaction layer which is formed in contact with or in the vicinity of the electrode system and contains at least a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the reaction layer further containing an additive such as phthalic acid.

摘要翻译： 本发明提供了一种高性能葡萄糖传感器，其可以显示对保存的高稳定性并且仅产生低的空白值。 葡萄糖传感器包括电绝缘基板，形成在基板上的电极系统和与电极系统接触或附近形成的反应层，并且至少含有辅酶是吡咯并喹啉的葡萄糖脱氢酶 醌，反应层还含有邻苯二甲酸等添加剂。

公开(公告)号：US06342364B1

公开(公告)日：2002-01-29

申请号：US09831091

申请日：2001-05-03

申请人： Motokazu Watanabe ,  Keiko Yugawa ,  Shiro Nankai

发明人： Motokazu Watanabe ,  Keiko Yugawa ,  Shiro Nankai

IPC分类号： C12Q160

CPC分类号： C12Q1/60 ,  C12Q1/005 ,  G01N33/92 ,  G01N2800/044 ,  Y10S435/817

摘要： The present invention provides a sensor that electrochemically determines cholesterol in low density lipoprotein by only one feed of a sample. The sensor has: an electrode system that is mounted on an electrically insulating base plate and includes at least a working electrode and a counter electrode; an enzyme layer formed on the base plate with the electrode system; and a reagent layer that is arranged before the enzyme layer in a sample solution supply path to the electrode system. The enzyme layer includes at least an oxidoreductase and an electron mediator. The reagent layer includes a reagent that depresses reactivity of cholesterol in lipoproteins other than the low density lipoprotein with the oxidoreductase, for example, a reagent that attaches to lipoproteins other than the low density lipoprotein to form a water-soluble complex.

摘要翻译： 本发明提供一种传感器，其通过仅一种样品的进料电化学地测定低密度脂蛋白中的胆固醇。 传感器具有：电极系统，其安装在电绝缘基板上，并且至少包括工作电极和对电极; 在所述基板上形成有电极系的酶层; 以及被配置在所述酶层之前的试剂溶液供给到所述电极系统的路径中的试剂层。 酶层至少包含氧化还原酶和电子介体。 试剂层包括抑制低密度脂蛋白以外的脂蛋白中的胆固醇与氧化还原酶的反应性的试剂，例如附着于低密度脂蛋白以外的脂蛋白的试剂，形成水溶性络合物。

公开(公告)号：US06656702B1

公开(公告)日：2003-12-02

申请号：US09345676

申请日：1999-06-30

申请人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Keiko Yugawa ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q132

CPC分类号： C12Q1/005

摘要： A biosensor that enables rapid and simple quantitation of a substrate with high accuracy and demonstrates an excellent preservation characteristic by best retaining enzyme activity is disclosed. The biosensor comprises an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on the base plate, and a reaction layer containing at least an enzyme and a sugar. The reaction layer may be formed on the electrode system. The enzyme may be selected from glucose oxidase, glucose dehydrogenase, and fructose dehydrogenase. The sugar is trehalose, sucrose, glycerol, mannitol, and ribose. If the enzyme selected is glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the use of trehalose is preferable.

摘要翻译： 公开了一种生物传感器，其能够高精度地快速和简单地定量基质，并通过最佳的保留酶活性表现出优异的保存特性。 生物传感器包括电绝缘基板，至少包括形成在基板上的工作电极和对电极的电极系统和至少含有酶和糖的反应层。 反应层可以形成在电极系统上。 酶可以选自葡萄糖氧化酶，葡萄糖脱氢酶和果糖脱氢酶。 糖是海藻糖，蔗糖，甘油，甘露糖醇和核糖。 如果选择的酶是辅酶是吡咯并喹啉醌的葡萄糖脱氢酶，则优选使用海藻糖。

公开(公告)号：US20050009102A1

公开(公告)日：2005-01-13

申请号：US10886640

申请日：2004-07-09

申请人： Tatsurou Kawamura ,  Keiko Yugawa ,  Akihito Kamei

发明人： Tatsurou Kawamura ,  Keiko Yugawa ,  Akihito Kamei

IPC分类号： G01N21/82 ,  G01N33/52 ,  G01N33/53 ,  G01N33/536 ,  G01N33/557

CPC分类号： G01N33/557

摘要： According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and/or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and/or minimum value is observed, and turbidity level.

摘要翻译： 根据本发明，可以在没有稀释步骤等的情况下实现样品溶液中的抗原的可测量的浓度范围的扩大。 样品溶液中含有的抗原浓度是根据观察浊度的瞬时现象，观察到最大值和/或最小值时的经过时间以及浊度的最大值和/或最小值来确定的。 水平。

公开(公告)号：US07241578B2

公开(公告)日：2007-07-10

申请号：US10441192

申请日：2003-05-20

申请人： Keiko Yugawa ,  Nobuyuki Shigetoh ,  Akihito Kamei ,  Tatsurou Kawamura

发明人： Keiko Yugawa ,  Nobuyuki Shigetoh ,  Akihito Kamei ,  Tatsurou Kawamura

IPC分类号： G01K33/53 ,  G01K33/536 ,  G01K33/557 ,  G01K33/577 ,  C07K16/18 ,  C12N5/20 ,  C12P21/08

CPC分类号： A61B5/415 ,  A61B5/20 ,  A61B10/0038 ,  A61B10/007 ,  C07K16/18 ,  G01N33/577 ,  G01N33/68

摘要： The immunoassay method of this invention measures the content of a subject substance in a sample. The method includes the steps of: (a) preparing a mixed solution by mixing the sample and an antibody solution including a first monoclonal antibody and a second monoclonal antibody capable of specifically binding to the subject substance; and (b) measuring an optical property of the mixed solution. The first monoclonal antibody is capable of binding to a first epitope of the subject substance, and the second monoclonal antibody is capable of binding to a second epitope of the subject substance different from the first epitope. Each of the first and second epitopes exists singly in the subject substance.

摘要翻译： 本发明的免疫测定方法测定样品中目标物质的含量。 该方法包括以下步骤：（a）通过混合样品和包含第一单克隆抗体和能够特异性结合本发明物质的第二单克隆抗体的抗体溶液来制备混合溶液; 和（b）测量混合溶液的光学性质。 第一单克隆抗体能够结合受试物质的第一表位，第二单克隆抗体能够结合不同于第一表位的受试物质的第二表位。 第一和第二表位中的每一个单独存在于主题物质中。

公开(公告)号：US07226777B2

公开(公告)日：2007-06-05

申请号：US10886640

申请日：2004-07-09

申请人： Tatsurou Kawamura ,  Keiko Yugawa ,  Akihito Kamei

发明人： Tatsurou Kawamura ,  Keiko Yugawa ,  Akihito Kamei

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： G01N33/557

摘要： According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and/or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and/or minimum value is observed, and turbidity level.

摘要翻译： 根据本发明，可以在没有稀释步骤等的情况下实现样品溶液中的抗原的可测量的浓度范围的扩大。 样品溶液中含有的抗原浓度是根据观察浊度的瞬时现象，观察到最大值和/或最小值时的经过时间以及浊度的最大值和/或最小值来确定的。 水平。

公开(公告)号：US07202041B2

公开(公告)日：2007-04-10

申请号：US10516067

申请日：2003-12-09

申请人： Akihito Kamei ,  Tatsurou Kawamura ,  Keiko Yugawa

发明人： Akihito Kamei ,  Tatsurou Kawamura ,  Keiko Yugawa

IPC分类号： G01N33/53 ,  G01N33/536 ,  G01N21/00 ,  C07K16/00

CPC分类号： G01N33/5306 ,  Y10S435/961 ,  Y10S436/805

摘要： The present invention relates to an immunoreaction measuring method for measuring an antigen or antibody contained as a subject substance in a sample, wherein the sample was mixed with at least one compound selected from the group consisting of dicarboxylic acids having a hydroxyl group, dicarboxylic acids having a double bond, straight-chain dicarboxylic acids expressed by the chemical formula (1): HOOC(CH2)nCOOH (n is an integer), and the salts of these dicarboxylic acids, and an antibody or antigen as a specifically binding substance capable of specifically binding to the subject substance, to obtain an acidic reaction solution, and then an antigen-antibody complex, generated by an antigen-antibody reaction of the subject substance with the specifically binding substance in the reaction solution was detected. This makes it possible to improve a measurement value and to relax a limitation of a measurement range due to a zone phenomenon that occurs in an antigen-excess region.

摘要翻译： 本发明涉及一种用于测量样品中作为主体物质的抗原或抗体的免疫反应测定方法，其中将样品与选自由具有羟基的二羧酸组成的组中选择的至少一种化合物混合， 双键，由化学式（1）表示的直链二羧酸：HOOC（CH 2 CH 2）n COOH（n为整数），其盐 这些二羧酸和作为特异性结合物质的能够与本发明物质特异性结合的抗体或抗原，获得酸性反应溶液，然后将抗原 - 抗体复合物通过本发明物质的抗原 - 抗体反应与 检测反应溶液中特异性结合物质。 这使得可以改善测量值并且放松由于在抗原过量区域中发生的区域现象引起的测量范围的限制。

公开(公告)号：US06174723B1

公开(公告)日：2001-01-16

申请号：US08982963

申请日：1997-12-02

申请人： Keiko Yugawa ,  Nobuyuki Sigetoh ,  Jinsei Miyazaki ,  Tadayasu Mitsumata

发明人： Keiko Yugawa ,  Nobuyuki Sigetoh ,  Jinsei Miyazaki ,  Tadayasu Mitsumata

IPC分类号： C12N506

CPC分类号： G01N33/946 ,  A61K47/60 ,  A61K47/643 ,  C07D451/12 ,  C07K16/16

摘要： A method for preparing a cocaine-protein conjugate easily by using a cocaine derivative having a methoxy carbonyl group and benzoyl group. This conjugate is useful for the detection of cocaine or cocaine derivatives. A monoclonal antibody, a monoclonal antibody producing cell line, and a method for producing the monoclonal antibody producing cell line by using the above cocaine-protein conjugate as an immunogen is also described. The method of the invention comprises preparing a cocaine-protein conjugate with a pyridyl dithiococaine derivative of Formula (I): where R is either H or R′; and where R′ is: wherein n=1 to 4 and Ph is a benzene ring, and wherein said monoclonal antibody detects 10−9 M cocaine; and which derivative binds to a protein by a disulfide bond; immunizing A/J mouse with the conjugate; fusing its spleen cell and a myeloma cell line; cloning the fusion to produce a monoclonal antibody producing cell line; culturing the cell line, and purifying a supernatant of the culture to form a monoclonal antibody to specifically bind to cocaine or cocaine derivatives.

摘要翻译： 通过使用具有甲氧基羰基和苯甲酰基的可卡因衍生物容易地制备可卡因 - 蛋白质结合物的方法。 该缀合物可用于检测可卡因或可卡因衍生物。 还描述了单克隆抗体，产生单克隆抗体的细胞系，以及通过使用上述可卡因蛋白质缀合物作为免疫原来制备产生单克隆抗体的细胞系的方法。 本发明的方法包括用式（I）的吡啶基二硫代可卡因衍生物制备可卡因蛋白质结合物：其中R是H或R'; 其中R'为：其中n = 1-4，Ph为苯环，其中所述单克隆抗体检测10-9M可卡因; 哪个衍生物通过二硫键与蛋白质结合; 用缀合物免疫A / J小鼠; 融合其脾细胞和骨髓瘤细胞系; 克隆融合物以产生产生单克隆抗体的细胞系; 培养细胞系，并纯化培养物的上清液以形成特异性结合可卡因或可卡因衍生物的单克隆抗体。

公开(公告)号：US20090093068A1

公开(公告)日：2009-04-09

申请号：US12330859

申请日：2008-12-09

申请人： Keiko Yugawa ,  Shinji Tanaka ,  Shin Ikeda

发明人： Keiko Yugawa ,  Shinji Tanaka ,  Shin Ikeda

IPC分类号： G01N33/53 ,  B01J19/00

CPC分类号： G01N33/536 ,  B01L3/508 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/0858 ,  B01L2400/0487 ,  G01N21/03 ,  G01N2021/0325

摘要： An immunosensor includes a base body (101) having a sample holding portion (102) which holds a test sample, a sample introducing port (103) which is communicated with the sample holding portion (102) and through which the test sample is introduced to the sample holding portion (102), a dried first reagent body (109) which contains an antibody to a material to be measured which is contained in the test sample, and a dried second reagent body (110) which contains polyethylene glycol, and in the sample holding portion (102), the first reagent body (109) is placed closer to the sample introducing port (103) than the second reagent body (110).

摘要翻译： 免疫传感器包括：具有保持试样的样品保持部（102）的基体（101），与试样保持部（102）连通的试样导入口（103），将试样插入其中 样品保持部（102），含有测试样品中包含的被测定材料的抗体的干燥的第一试剂体（109）和含有聚乙二醇的干燥后的第二试剂体（110） 样品保持部（102），第一试剂体（109）比第二试剂体（110）更靠近试样导入口（103）。