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公开(公告)号：US4503147A
公开(公告)日：1985-03-05
申请号：US435316
申请日：1982-10-19
CPC分类号： C12N9/0022 , C12Q1/26 , Y10S435/829 , Y10S435/832
摘要： A monomethylamine-oxidizing enzyme can be obtained by cultivating in a medium a strain which belongs to Genus Bacillus and has an ability to produce a monomethylamine-oxidizing enzyme. This enzyme exhibits several beneficial properties including the ability to oxidatively deaminate the amino group of monomethylamine to produce formaldehyde, ammonia, and hydrogen peroxide. The enzyme exhibits a high substrate specificity for monomethylamine, ethylamine, and n-proplyamine while showing no substrate specificity for benzylamine, dimethylamine, trimethylamine, ethylenediamine and tryamine. In addition, the enzyme is stable through an elevated temperature range permitting faster reaction rates and therefore a shorter overall quantitative evaluation. Another property includes a low Km value which allows smaller quantities of the enzyme to be employed per sample.
摘要翻译： 通过在培养基中培养属于芽孢杆菌属的菌株并具有产生单甲胺氧化酶的能力，可以获得单甲胺氧化酶。 该酶显示出几种有益的性质，包括使一甲胺的氨基氧化脱氨以产生甲醛，氨和过氧化氢的能力。 该酶对单甲胺，乙胺和正丙胺显示出高的底物特异性，同时对苄胺，二甲胺，三甲胺，乙二胺和试胺没有底物特异性。 此外，酶在升高的温度范围内是稳定的，允许更快的反应速率，因此较短的总体定量评估。 另一种性质包括低Km值，其允许每个样品使用较少量的酶。
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公开(公告)号：US4247632A
公开(公告)日：1981-01-27
申请号：US37028
申请日：1979-05-08
CPC分类号： C12Q1/34 , C12N9/78 , G01N2333/978 , Y10S435/829
摘要： A novel methylguanidine-decomposing enzyme can be obtained by cultivating in a medium a bacterium belonging to Genus Alcaligenes and having an ability to produce a methylguanidine-decomposing enzyme. This methylguanidine-decomposing enzyme has an ability to decompose methylguanidine into methylamine and urea. Its optimum pH range is 10.9-12.3 and its stable pH range is 5.0-10.6.
摘要翻译： 通过在属于产碱杆菌属的细菌的培养基中培养并具有产生甲基胍分解酶的能力，可以获得新的甲基胍分解酶。 该甲基胍分解酶具有将甲基胍分解成甲胺和尿素的能力。 其最适pH范围为10.9-12.3，其稳定pH范围为5.0-10.6。
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