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公开(公告)号:US20100317075A1
公开(公告)日:2010-12-16
申请号:US12840026
申请日:2010-07-20
申请人: NORIYUKI KIZAKI , MIHO YANO , MASAHIRO FUNAKI , TERUAKI TAKESUE , YOSHIHIKO YASOHARA , SOUICHI MORIKAWA , TAKAHISA NAKAI , MICHIHIKO KATAOKA , SAKAYU SHIMIZU
发明人: NORIYUKI KIZAKI , MIHO YANO , MASAHIRO FUNAKI , TERUAKI TAKESUE , YOSHIHIKO YASOHARA , SOUICHI MORIKAWA , TAKAHISA NAKAI , MICHIHIKO KATAOKA , SAKAYU SHIMIZU
CPC分类号: C12P13/02 , C12N9/0006 , C12P41/002
摘要: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant.Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
摘要翻译: 本发明涉及具有不对称还原(3S)-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁酮的活性以产生(2R,3S)-1-氯-3-叔丁氧基羰基氨基-4- 从属于Ogataea的微生物分离的丁氧基羰基氨基-4-苯基-2-丁醇,编码多肽的DNA和产生多肽的转化体。 本发明还涉及利用多肽或转化体制备(2R,3S)-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇的方法。 使用本发明的多肽或转化体,可以有效地制备光学活性醇如(2R,3S)-1-氯-3-叔丁氧基羰基氨基-4-苯基-2-丁醇等。