公开(公告)号：US06521304B1

公开(公告)日：2003-02-18

申请号：US09331292

申请日：1999-06-18

申请人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

发明人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

IPC分类号： F21K206

CPC分类号： C09K11/07 ,  F21K2/06

摘要： The present invention relates to luminescent playthings such as candles, table lights, pen lights, illumination, illuminating playthings for camp, illuminating playthings for night fishing, fish-gathering lamps, safety candles, neon lights, luminescent inks, luminescent pens, luminescent coatings or luminescent writing implements, in which a soft light of an indescribable tone of color not obtainable from flames of conventional candles is emitted via seawater, lake and marsh water, river water, ground water, tap water or mineral drinking water by utilizing 3 components of luciferin, luciferase and ATP or 4 components of these components plus a metal salt, out of components necessary for bioluminescence in fireflies.

摘要翻译： 本发明涉及诸如蜡烛，台灯，笔灯，照明，营地的照明玩具，夜间钓鱼的照明玩具，聚光灯，安全蜡烛，霓虹灯，发光油墨，发光笔，发光涂层或发光的发光玩具 发光书写工具，其中可以通过海水，湖泊和沼泽水，河水，地下水，自来水或矿物饮用水通过利用荧光素3的三种成分从普通蜡烛的火焰中获得难以形容的色调的柔和光线 ，荧光素酶和ATP或这些组分的4种组分加上金属盐，不仅仅是萤火虫生物发光所必需的组分。

公开(公告)号：US5352598A

公开(公告)日：1994-10-04

申请号：US759814

申请日：1991-08-29

申请人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

发明人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

IPC分类号： C12N9/02 ,  C12N1/00

CPC分类号： C12N9/0069 ,  C12Y113/12007 ,  Y10S435/815 ,  Y10S435/816

摘要： A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.

摘要翻译： 公开了一种来自Luciola lateralis的纯化的萤光素酶。 该酶的特征在于具有以下特性：最佳pH范围为7.5至9.5，最适温度范围为0℃至50℃，并且该酶不作用于ADP，CTP，UTP和GTP。 通过使用包括凝胶过滤色谱法，羟基磷灰石柱色谱法和三（羟基）氨基甲烷 - 氢氯酸缓冲液的方法纯化酶。

公开(公告)号：US5182202A

公开(公告)日：1993-01-26

申请号：US742477

申请日：1991-08-05

申请人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

发明人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

IPC分类号： C12N9/02

CPC分类号： C12N9/0069 ,  C12Y113/12007

摘要： Disclosed is a purified luciferase and a method for making it. The luciferase is obtained from Luciola cruciata. The luciferase has a pH range for stabililty of 6.5-9.0 and a optimum pH range of 8.0-9.5. The enzyme does not act on ADP, CTP, UTP and GTP.

摘要翻译： 公开了一种纯化的荧光素酶及其制备方法。 萤光素酶得自Luciola cruciata。 萤光素酶的稳定pH范围为6.5-9.0，最适pH范围为8.0-9.5。 该酶不对ADP，CTP，UTP和GTP起作用。

公开(公告)号：US07070948B1

公开(公告)日：2006-07-04

申请号：US10088524

申请日：2000-09-29

申请人： Ryoichi Sakaue ,  Ayumi Arai ,  Naoki Kajiyama ,  Yasuji Koyama

发明人： Ryoichi Sakaue ,  Ayumi Arai ,  Naoki Kajiyama ,  Yasuji Koyama

IPC分类号： C12Q1/37 ,  C01N33/53

CPC分类号： C12Q1/26 ,  C12Q1/37 ,  G01N30/02 ,  Y10S435/975

摘要： Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an α-glycated peptide, particularly preferably an α-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.

摘要翻译： 基于与常规酶法不同的原理，本发明提供了一种通过简单的方法在短时间内和高精度测定糖基化蛋白质的新方法和用于测定的试剂盒 用于该方法。 用于测定样品中糖化蛋白质的方法是通过用蛋白酶处理含糖基化蛋白的样品来实现的，以从糖基化蛋白质释放糖基化肽，优选α-糖化肽，特别优选α-糖化二肽，从而允许 氧化酶与释放的糖化肽反应，并测定所产生的过氧化氢。

公开(公告)号：US5330906A

公开(公告)日：1994-07-19

申请号：US76042

申请日：1993-06-15

申请人： Naoki Kajiyama ,  Eiichi Nakano

发明人： Naoki Kajiyama ,  Eiichi Nakano

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12Q1/68 ,  C12N15/70

CPC分类号： C12N9/0069 ,  C12Q1/6897 ,  C12Y113/12007

摘要： The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.

摘要翻译： 本发明提供了工业上有用的荧光素酶。 本发明的突变型荧光素酶是通过培养属于埃希氏杆菌属的微生物，其生产含有萤火虫突变萤光素酶基因的重组DNA。 突变萤光素酶可以产生不能由野生型荧光素酶产生的红色，橙色或绿色的光。 突变荧光素酶可用于在有色溶液（如红色（如血液），橙色或绿色）中准确测定ATP，其中野生型荧光素酶未提供可靠的结果。

公开(公告)号：US5219737A

公开(公告)日：1993-06-15

申请号：US675211

申请日：1991-03-26

申请人： Naoki Kajiyama ,  Eiichi Nakano

发明人： Naoki Kajiyama ,  Eiichi Nakano

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12Q1/68

CPC分类号： C12N9/0069 ,  C12Q1/6897 ,  C12Y113/12007

摘要： The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.

公开(公告)号：US5229285A

公开(公告)日：1993-07-20

申请号：US903047

申请日：1992-06-23

申请人： Naoki Kajiyama ,  Eiichi Nakano

发明人： Naoki Kajiyama ,  Eiichi Nakano

IPC分类号： C12N9/02 ,  C12N15/53

CPC分类号： C12N9/0069 ,  C12Y113/12007

摘要： The present invention relates to thermostable luciferase of firefly wherein an amino acid at the 217-position of the amino acid sequence of wild-type firefly luciferase or an amino acid equivalent to the amino acid at the 217-position of luciferase of GENJI firefly or HEIKE firefly is converted into a hydrophobic amino acid, a gene encoding said thermostable luciferase, a vector comprising the gene encoding said thermostable luciferase inserted therein, and a process for the preparation of thermostable firefly luciferase comprising use of said vector.

公开(公告)号：US20110003361A1

公开(公告)日：2011-01-06

申请号：US12846153

申请日：2010-07-29

申请人： Keiko KUROSAWA ,  Kozo Hirokawa ,  Naoki Kajiyama

发明人： Keiko KUROSAWA ,  Kozo Hirokawa ,  Naoki Kajiyama

IPC分类号： C12N9/06

CPC分类号： C12N9/0022

摘要： The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase.A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.

摘要翻译： 本发明的目的是提供具有优异的物理化学性质的新型果糖基氧化酶，例如可用作临床诊断用酶的稳定性，以及提供生产果糖基氧化酶的方法的目的。 本发明提供了具有用作临床诊断用酶的物理化学特性的新型果糖基氧化酶，以及生产新型果糖基氧化酶的方法，该方法包括：培养能够在培养基中产生氧化酶的微生物; 并从培养物中收集氧化酶。 此外，本发明提供了编码新型果糖基肽氧化酶的果糖基肽氧化酶基因，其中将基因插入载体DNA中的重组DNA，以及生产新型果糖基氧化酶的方法，该方法包括：在培养基中培养， 包含该基因的转化体或转导体; 并从培养物中收集新颖的果糖基氧化酶。

公开(公告)号：US20070269860A1

公开(公告)日：2007-11-22

申请号：US11627484

申请日：2007-01-26

申请人： Keisuke FURUKAWA ,  Naoki Kajiyama

发明人： Keisuke FURUKAWA ,  Naoki Kajiyama

IPC分类号： C07H21/04

CPC分类号： C12N9/0034

摘要： (1) A modified sarcosine oxidase with improved stability in the acidic range compared to a wild-type sarcosine oxidase. (2) A sarcosine oxidase gene encoding a modified sarcosine oxidase of the following (a), (b), or (c): (a) protein composed of the amino acid sequence represented by SEQ ID NO: 1 (b) protein composed of an amino acid sequence wherein one or some amino acid(s) are deleted, substituted, or added from the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity (c) protein composed of an amino acid sequence which shows 80% or more homology to the amino acid sequence represented by SEQ ID NO: 1, and which has sarcosine oxidase activity According to the present invention, sarcosine oxidases, in particular sarcosine oxidases which show optimal pH and high activity in the slightly acidic range and have improved stability can be prepared efficiently, thus making the invention industrially useful.

摘要翻译： （1）与野生型肌氨酸氧化酶相比，在酸性范围内具有改善的稳定性的改性肌氨酸氧化酶。 （2）编码以下（a），（b）或（c）的修饰肌氨酸氧化酶的肌氨酸氧化酶基因：（a）由SEQ ID NO：1（b）表示的氨基酸序列组成的蛋白质， 的氨基酸序列，其中一个或一些氨基酸被从SEQ ID NO：1所示的氨基酸序列中缺失，取代或添加，并具有肌氨酸氧化酶活性（c）由氨基酸序列组成的蛋白质 其显示与SEQ ID NO：1所示的氨基酸序列具有80％或更高的同源性，并且其具有肌氨酸氧化酶活性根据本发明，肌氨酸氧化酶，特别是在微酸性中显示最佳pH和高活性的肌氨酸氧化酶 可有效制备稳定性，从而使本发明在工业上有用。

公开(公告)号：US20070037243A1

公开(公告)日：2007-02-15

申请号：US10572052

申请日：2004-09-13

申请人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

发明人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

IPC分类号： C12Q1/26 ,  C12P21/06

CPC分类号： C12P21/02 ,  C12P21/06

摘要： The present invention relates to a method for producing α-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of α-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the α-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing α-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of α-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of α-glycated dipeptide is provided, which enables to determine the amount of α-glycated dipeptide in a highly precise manner within a short time period.

摘要翻译： 本发明涉及α-糖基化二肽的制备方法，其包括使蛋白酶作用于N-末端糖基化肽或N-末端糖基化蛋白质。 本发明还涉及确定α-糖化二肽量的方法，其包括使果糖基肽氧化酶作用于通过上述方法获得的α-糖化二肽，然后测定由此产生的过氧化氢的量。 根据本发明，提供了α-糖化二肽的制备方法，能够从糖化蛋白质或糖化肽中简单，快速，有效地生产α-糖化二肽。 此外，根据本发明，提供了用于测定α-糖化二肽量的方法，其能够在短时间内以高度精确的方式测定α-糖化二肽的量。