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    Purified luciferase from luciola lateralis
    1.
    发明授权
    Purified luciferase from luciola lateralis 失效
    来自luciola lateralis的纯化萤光素酶

    公开(公告)号：US5352598A

    公开(公告)日：1994-10-04

    申请号：US759814

    申请日：1991-08-29

    申请人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    发明人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N1/00

    CPC分类号： C12N9/0069 ,  C12Y113/12007 ,  Y10S435/815 ,  Y10S435/816

    摘要： A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.

    摘要翻译： 公开了一种来自Luciola lateralis的纯化的萤光素酶。 该酶的特征在于具有以下特性：最佳pH范围为7.5至9.5，最适温度范围为0℃至50℃，并且该酶不作用于ADP，CTP，UTP和GTP。 通过使用包括凝胶过滤色谱法，羟基磷灰石柱色谱法和三（羟基）氨基甲烷 - 氢氯酸缓冲液的方法纯化酶。

    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member
    3.
    发明授权
    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member 失效
    发光工具，其辅助构件和保存在工具中使用的生物发光组合物和辅助构件的方法

    公开(公告)号：US06521304B1

    公开(公告)日：2003-02-18

    申请号：US09331292

    申请日：1999-06-18

    申请人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    发明人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    IPC分类号： F21K206

    CPC分类号： C09K11/07 ,  F21K2/06

    摘要： The present invention relates to luminescent playthings such as candles, table lights, pen lights, illumination, illuminating playthings for camp, illuminating playthings for night fishing, fish-gathering lamps, safety candles, neon lights, luminescent inks, luminescent pens, luminescent coatings or luminescent writing implements, in which a soft light of an indescribable tone of color not obtainable from flames of conventional candles is emitted via seawater, lake and marsh water, river water, ground water, tap water or mineral drinking water by utilizing 3 components of luciferin, luciferase and ATP or 4 components of these components plus a metal salt, out of components necessary for bioluminescence in fireflies.

    摘要翻译： 本发明涉及诸如蜡烛，台灯，笔灯，照明，营地的照明玩具，夜间钓鱼的照明玩具，聚光灯，安全蜡烛，霓虹灯，发光油墨，发光笔，发光涂层或发光的发光玩具 发光书写工具，其中可以通过海水，湖泊和沼泽水，河水，地下水，自来水或矿物饮用水通过利用荧光素3的三种成分从普通蜡烛的火焰中获得难以形容的色调的柔和光线 ，荧光素酶和ATP或这些组分的4种组分加上金属盐，不仅仅是萤火虫生物发光所必需的组分。

    Mutant luciferase of a firefly
    6.
    发明授权
    Mutant luciferase of a firefly 失效
    萤火虫的突变萤光素酶

    公开(公告)号：US5330906A

    公开(公告)日：1994-07-19

    申请号：US76042

    申请日：1993-06-15

    申请人： Naoki Kajiyama ,  Eiichi Nakano

    发明人： Naoki Kajiyama ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N15/53 ,  C12Q1/68 ,  C12N15/70

    CPC分类号： C12N9/0069 ,  C12Q1/6897 ,  C12Y113/12007

    摘要： The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.

    摘要翻译： 本发明提供了工业上有用的荧光素酶。 本发明的突变型荧光素酶是通过培养属于埃希氏杆菌属的微生物，其生产含有萤火虫突变萤光素酶基因的重组DNA。 突变萤光素酶可以产生不能由野生型荧光素酶产生的红色，橙色或绿色的光。 突变荧光素酶可用于在有色溶液（如红色（如血液），橙色或绿色）中准确测定ATP，其中野生型荧光素酶未提供可靠的结果。

    Method for preparation of a recombinant DNA phage
    10.
    发明授权
    Method for preparation of a recombinant DNA phage 失效
    重组DNA噬菌体的制备方法

    公开(公告)号：US4348478A

    公开(公告)日：1982-09-07

    申请号：US87167

    申请日：1979-10-22

    申请人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

    发明人： Eiichi Nakano ,  Tsutomu Masuda ,  Narimasa Saito ,  Danji Fukushima

    IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P19/34 ,  C12R1/91

    CPC分类号： C12N15/00

    摘要： A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.

    摘要翻译： 一种制备新的重组DNA的方法，其包括（1）用核酸内切酶切割温和的噬菌体DNA，所述温带噬菌体DNA具有参与噬菌体DNA复制的DNA片段中不具有内切核酸酶敏感区域，并将噬菌体DNA整合到 宿主染色体，但至少在DNA片段中携带外壳蛋白生产的遗传信息和携带预期遗传信息的另一个DNA，（2）向裂解DNA的混合物中加入DNA连接酶，和（3）从混合物中回收噬菌体 通过用携带预期信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段，缺失其外壳蛋白产生能力的DNA。