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    DATA ANALYSIS OF DNA SEQUENCES
    1.
    发明申请
    DATA ANALYSIS OF DNA SEQUENCES 审中-公开
    DNA序列的数据分析

    公开(公告)号：US20120173153A1

    公开(公告)日：2012-07-05

    申请号：US13332242

    申请日：2011-12-20

    申请人： Navin Elango ,  Joseph F. Petolino ,  Shreedharan Sriram ,  Lakshmi Sastry-Dent

    发明人： Navin Elango ,  Joseph F. Petolino ,  Shreedharan Sriram ,  Lakshmi Sastry-Dent

    IPC分类号： G06F19/22

    CPC分类号： G16B30/00

    摘要： Systems and methods for data analysis are provided. In one embodiment, a method may be provided for analysis comprising electronically receiving sequence data related to a plurality of sequences and a reference sequence, associating the sequence data with one of at least two groups, identifying a plurality of high quality read sequences from among the plurality of sequences, extracting a plurality of unique read sequences from the plurality of high quality read sequences, and aligning the plurality of unique read sequences against the reference sequence data corresponding to a reference sample. The method may further identify mutations in a targeted location, display the targeted mutations, and prioritize the technologies that caused the mutations according to their efficiency. In one example, the systems and methods are used to characterize the activity of several ZFN candidates.

    摘要翻译： 提供了数据分析的系统和方法。 在一个实施例中，可以提供一种用于分析的方法，包括电子地接收与多个序列相关的序列数据和参考序列，将序列数据与至少两个组中的一个相关联，从多个序列中识别多个高质量读取序列 多个序列，从所述多个高质量读取序列提取多个唯一读取序列，以及将所述多个唯一读取序列与对应于参考样本的参考序列数据对准。 该方法可以进一步鉴定目标位置中的突变，显示目标突变，并根据其效率对引起突变的技术进行优先化。 在一个示例中，系统和方法用于表征若干ZFN候选者的活动。

    EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS
    2.
    发明申请
    EXCISION OF TRANSGENES IN GENETICALLY MODIFIED ORGANISMS 有权
    在遗传改良机构中实施转基因

    公开(公告)号：US20110191877A1

    公开(公告)日：2011-08-04

    申请号：US13011666

    申请日：2011-01-21

    申请人： Sean M. Russell ,  Joseph F. Petolino

    发明人： Sean M. Russell ,  Joseph F. Petolino

    IPC分类号： A01H1/00 ,  A01H1/06 ,  A01H5/00 ,  C07H21/00 ,  C12N5/10

    CPC分类号： C12N15/8213 ,  A01H1/02 ,  C12N9/22 ,  C12N15/8241 ,  C12N15/8265

    摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

    摘要翻译： 一种删除植物中DNA区域的方法。 在一些实施方案中，所述方法包括用核酸分子转化植物，其中所述核酸分子编码与一个或多个组织特异性启动子可操作地连接的一个或多个锌指核酸酶（ZFN），例如， 花粉特异性启动子。 方法包括在植物中切除天然基因。 因此，在一些实施方案中，ZFN被设计为识别与天然植物基因相邻的序列。 在进一步的实施方案中，ZFN在发育阶段特异性启动子的控制下表达，使得例如在相对较晚的发育阶段在植物中特异性切除核酸序列。 包括用于实施所公开的方法的核酸分子和通过该方法生产的植物。

    Increasing the anther culturability of maize
    5.
    发明授权
    Increasing the anther culturability of maize 失效
    增加玉米的花药培养

    公开(公告)号：US5602310A

    公开(公告)日：1997-02-11

    申请号：US221297

    申请日：1994-03-31

    申请人： Joseph F. Petolino

    发明人： Joseph F. Petolino

    IPC分类号： A01H1/02 ,  A01H4/00 ,  A01H5/00 ,  A01H1/04 ,  C12N15/02

    CPC分类号： A01H4/00 ,  A01H1/02

    摘要： This invention provides a process for producing germplasm of plant species exhibiting enhanced response to anther culture. The anther culture procedure itself is used as a selection criterion for genes favoring in vitro androgenesis. After subjecting anthers to standard anther culture regeneration procedures, the regenerated plants are intermated and self-pollinated to generate valuable genetic variability for improved culture response. The transfer of increased anther culturability to other selected germplasm is also possible.

    摘要翻译： 本发明提供了一种用于生产对花药培养具有增强反应的植物物种的种质的方法。 花药培养程序本身被用作有利于体外雄激素发生的基因的选择标准。 在使花药进行标准的花药培养再生程序后，再生植物被间接和自花授粉以产生有价值的遗传变异性以改善培养反应。 将花药培养增加转移到其他选择的种质也是可能的。

    Increasing the anther culturability of maize
    9.
    发明授权
    Increasing the anther culturability of maize 失效
    增加玉米的花药培养

    公开(公告)号：US5306864A

    公开(公告)日：1994-04-26

    申请号：US839024

    申请日：1992-02-18

    申请人： Joseph F. Petolino

    发明人： Joseph F. Petolino

    IPC分类号： A01H1/02 ,  A01H4/00 ,  A01H5/00 ,  A01H1/04

    CPC分类号： A01H4/00 ,  A01H1/02

    摘要： This invention provides a process for producing germplasm of plant species exhibiting enhanced response to anther culture. The anther culture procedure itself is used as a selection criterion for genes favoring in vitro androgenesis. After subjecting anthers to standard anther culture regeneration procedures, the regenerated plants are intermated and self-pollinated to generate valuable genetic variability for improved culture response. The transfer of increased anther culturability to other selected germplasm is also possible.

    摘要翻译： 本发明提供了一种用于生产对花药培养具有增强反应的植物物种的种质的方法。 花药培养程序本身被用作有利于体外雄激素发生的基因的选择标准。 在使花药进行标准的花药培养再生程序后，再生植物被间接和自花授粉以产生有价值的遗传变异性以改善培养反应。 将花药培养增加转移到其他选择的种质也是可能的。