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    Biotic and abiotic stress tolerance in plants
    2.
    发明授权
    Biotic and abiotic stress tolerance in plants 有权
    植物中的生物和非生物胁迫耐受性

    公开(公告)号：US08030546B2

    公开(公告)日：2011-10-04

    申请号：US12077535

    申请日：2008-03-17

    申请人： T. Lynne Reuber ,  Oliver Ratcliffe ,  Karen S. Century ,  Neal I. Gutterson ,  Roger Canales ,  Emily L. Queen

    发明人： T. Lynne Reuber ,  Oliver Ratcliffe ,  Karen S. Century ,  Neal I. Gutterson ,  Roger Canales ,  Emily L. Queen

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N5/14

    CPC分类号： C07K14/415 ,  C12N15/8261 ,  Y02A40/146

    摘要： Transcription factor polynucleotides and polypeptides incorporated into nucleic acid constructs, including expression vectors, have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these constructs have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include, for example, salt, hyperosmotic stress, water deficit, heat, cold, drought, or low nutrient conditions.

    摘要翻译： 掺入核酸构建体（包括表达载体）的转录因子多核苷酸和多肽已经被引入植物中并被异位表达。 已经显示用许多这些构建体转化的转基因植物对疾病（在一些情况下，多于一种病原体）更耐受，或更耐受非生物胁迫（在某些情况下，对多于一种非生物胁迫）。 非生物胁迫可包括例如盐，高渗胁迫，缺水，热，冷，干旱或低营养条件。

    Transcriptional regulation of plant disease tolerance
    4.
    发明授权
    Transcriptional regulation of plant disease tolerance 有权
    植物病害耐受性的转录调控

    公开(公告)号：US08912394B2

    公开(公告)日：2014-12-16

    申请号：US10903236

    申请日：2004-07-30

    申请人： Karen S. Century ,  Emily L. Queen ,  T. Lynne Reuber ,  Oliver Ratcliffe ,  Roger D. Canales ,  Neal I. Gutterson

    发明人： Karen S. Century ,  Emily L. Queen ,  T. Lynne Reuber ,  Oliver Ratcliffe ,  Roger D. Canales ,  Neal I. Gutterson

    IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8273 ,  C12N15/8282

    摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including disease stress and abiotic stress tolerance, as compared to wild-type or control plants. The invention also pertains to expression systems that may be used to regulate these transcription factor polynucleotides, providing constitutive, transient, inducible and tissue-specific regulation.

    摘要翻译： 本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同源物，以及与野生型相比，使用多核苷酸和多肽产生具有有利性质的转基因植物（包括疾病胁迫和非生物胁迫耐受性）的方法 型或对照植物。 本发明还涉及可用于调节这些转录因子多核苷酸的表达系统，提供组成型，瞬时，诱导型和组织特异性调节。

    STRONG ACTIVATION DOMAIN
    8.
    发明申请
    STRONG ACTIVATION DOMAIN 审中-公开
    强活动域

    公开(公告)号：US20110126326A1

    公开(公告)日：2011-05-26

    申请号：US13000488

    申请日：2009-06-26

    申请人： Shiv Tiwari ,  Roger Canales ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver Ratcliffe

    发明人： Shiv Tiwari ,  Roger Canales ,  T. Lynne Reuber ,  Karen S. Century ,  Oliver Ratcliffe

    IPC分类号： A01H5/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07H21/00 ,  C12N5/10 ,  C12N15/82

    摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the “EDLL domain” and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.

    摘要翻译： 从拟南芥蛋白质乙烯响应因子98（AtERF98）中鉴定出新的和强的转录激活结构域。 该领域已经被指定为“EDLL结构域”，并且具有许多高度保守的氨基酸残基，其在来自植物的AtERF98家族的整个成员中发现，包括单子叶植物和雌蕊直系同源物。 当EDLL结构域与植物和酵母的转录因子融合时，显示其具有高度的活性，并且还显示具有与广泛使用的衍生自单纯疱疹的VP16活化区域相当的活化潜力。 当通过序列特异性DNA结合蛋白或通过蛋白质 - 蛋白质相互作用靶向基因启动子时，EDLL结构域也是活性的。 与其他已知的活化结构域（如VP16和GAL4）不同，EDLL结构域的大小相对较小，并且是植物来源的，因此作为在转基因食品作物中应用的强转录激活工具是有利的。