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公开(公告)号：US20240287485A1
公开(公告)日：2024-08-29
申请号：US18596176
申请日：2024-03-05
Applicant: Oxford Nanopore Technologies PLC
Inventor： Andrew John Heron , Rebecca Victoria Bowen , Mark John Bruce , Lakmal Nishantha Jayasinghe , Joseph Hargreaves Lloyd , Szabolcs Soeroes , Elizabeth Jayne Wallace , Christopher Peter Youd
IPC: C12N9/14 , C12Q1/68 , C12Q1/6869
CPC classification number: C12N9/14 , C12Q1/68 , C12Q1/6869 , C12Y306/04012
Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
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公开(公告)号：US11965183B2
公开(公告)日：2024-04-23
申请号：US17500831
申请日：2021-10-13
Applicant: Oxford Nanopore Technologies PLC
Inventor： Andrew John Heron , Rebecca Victoria Bowen , Mark Bruce , Lakmal Jayasinghe , Joseph Hargreaves Lloyd , Szabolcs Soeroes , Elizabeth Jayne Wallace , Christopher Peter Youd
IPC: C12N9/14 , C12Q1/68 , C12Q1/6869
CPC classification number: C12N9/14 , C12Q1/68 , C12Q1/6869 , C12Y306/04012 , C12Q1/6869 , C12Q2521/513 , C12Q2565/631
Abstract: The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
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公开(公告)号：US20230227799A1
公开(公告)日：2023-07-20
申请号：US18047589
申请日：2022-10-18
Applicant: Oxford Nanopore Technologies PLC
Inventor： Andrew John Heron , James Anthony Clarke , Ruth Moysey , Elizabeth Jayne Wallace , Mark John Bruce , Lakmal Jayasinghe , Domenico Caprotti , Szabolcs Soeroes , Luke McNeill , David Antoni Alves , Rebecca Victoria Bowen , John Milton
IPC: C12N9/14 , C12N9/90 , C12Q1/6827
CPC classification number: C12N9/14 , C12Y306/04012 , C12N9/90 , C12Y599/01003 , C12Y599/01002 , C12Q1/6827 , C07K2319/80
Abstract: The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
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公开(公告)号：US20240060126A1
公开(公告)日：2024-02-22
申请号：US18341906
申请日：2023-06-27
Applicant: Oxford Nanopore Technologies PLC
Inventor： Lakmal Nishantha Jayasinghe , Elizabeth Jayne Wallace , Jonathan Bankes Pugh , Richard George Hambley , Neil Roger Wood , Clive Gavin Brown , James White , Andrew John Heron , Mark John Bruce , Christopher Peter Youd , Rebecca Victoria Bowen
IPC: C12Q1/6869 , C07K14/35
CPC classification number: C12Q1/6869 , C07K14/35
Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins
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公开(公告)号：US20230227903A1
公开(公告)日：2023-07-20
申请号：US18010826
申请日：2021-06-18
Applicant: Oxford Nanopore Technologies PLC
Inventor： Rebecca Victoria Bowen , Clive Gavin Brown , Mark John Bruce , Daniel Ryan Garalde , James Edward Graham , Andrew John Heron , Etienne Raimondeau , James White , Christopher Peter Youd
IPC: C12Q1/6869
CPC classification number: C12Q1/6869
Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.
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公开(公告)号：US20220389481A1
公开(公告)日：2022-12-08
申请号：US17778204
申请日：2020-11-20
Applicant: Oxford Nanopore Technologies PLC
Inventor： Andrew John Heron , Rebecca Victoria Bowen , Clive Gavin Brown
IPC: C12Q1/6806 , C12Q1/6869
Abstract: Provided herein is a method of sequencing a target double stranded nucleic acid. The method comprises contacting the double stranded nucleic acid with a reagent as described herein to form a construct and sequencing the construct using a single-molecule sequencing technique as described herein. Associated products and kits are further provided.
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公开(公告)号：US20240417704A1
公开(公告)日：2024-12-19
申请号：US18762324
申请日：2024-07-02
Applicant: Oxford Nanopore Technologies PLC
Inventor： Andrew John Heron , James Anthony Clarke , Ruth Moysey , Elizabeth Jayne Wallace , Mark John Bruce , Lakmal Nishantha Jayasinghe , Domenico Caprotti , Szabolcs Soeroes , Luke McNeill , David Antoni Alves , Rebecca Victoria Bowen , John Milton
IPC: C12N9/14 , C12N9/90 , C12Q1/6827
Abstract: The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
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公开(公告)号：US20240240245A1
公开(公告)日：2024-07-18
申请号：US17612606
申请日：2020-05-22
Applicant: Oxford Nanopore Technologies plc
Inventor： Andrew John Heron , Mark John Bruce , Rebecca Victoria Bowen , Luke Alexander McNeill , Simon Rafael Villarreal , Samuel John Martin , Rebecca Anne Stafford-Allen
IPC: C12Q1/6869 , C12Q1/6806
CPC classification number: C12Q1/6869 , C12Q1/6806
Abstract: Provided herein is a method of loading a motor protein onto a polynucleotide adapter. Also provided are polynucleotide adapters and kits comprising such adapters. The adapters find use in characterising analytes such as polynucleotides in methods in which the polynucleotide moves in respect of a nanopore.
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公开(公告)号：US20240076729A9
公开(公告)日：2024-03-07
申请号：US18010826
申请日：2021-06-18
Applicant: Oxford Nanopore Technologies PLC
Inventor： Rebecca Victoria Bowen , Clive Gavin Brown , Mark John Bruce , Daniel Ryan Garalde , James Edward Graham , Andrew John Heron , Etienne Raimondeau , James White , Christopher Peter Youd
IPC: C12Q1/6869
CPC classification number: C12Q1/6869
Abstract: Provided herein is a method of characterising a target polynucleotide as it moves with respect to a nanopore using a motor protein. Also provided are polynucleotide adapters and kits comprising such adapters. The methods, kits and adapters find use in characterising polynucleotides, for example in sequencing.
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公开(公告)号：US20240200128A1
公开(公告)日：2024-06-20
申请号：US18352701
申请日：2023-07-14
Applicant: Oxford Nanopore Technologies PLC
Inventor： Andrew John Heron , James Edward Graham , Richard Alexander Gutierrez , Rebecca Victoria Bowen , James White , Clive Gavin Brown , Daniel George Fordham
IPC: C12Q1/6869 , C12Q1/6876 , G01N33/487
CPC classification number: C12Q1/6869 , C12Q1/6876 , G01N33/48721
Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore: (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.
