公开(公告)号：US20160146786A1

公开(公告)日：2016-05-26

申请号：US14898064

申请日：2014-06-26

申请人： PHYLOGICA LIMITED

发明人： Richard Hopkins ,  Katrin Hoffmann ,  Tatjana Heinrich ,  Paula Cunningham ,  Paul Watt ,  Nadia Milech

IPC分类号： G01N33/50

CPC分类号： G01N33/5035 ,  C12Q1/25 ,  G01N33/52 ,  G01N33/68 ,  G01N2333/9015 ,  G01N2440/32 ,  G01N2500/10

摘要： This disclosure provides a method of isolating peptides having cell-penetrating function, wherein the peptides are detected as biotinylated molecules only following their translocation through the cell membrane. The disclosure also provides methods for validating the cell-penetrating function of the peptides, or that may be employed in their own right to isolate such peptides, wherein the peptides are detectable by virtue of their ability to transport a detectable cargo into the cytoplasm, such as a cargo toxin or a fragment of a green fluorescent protein (GFP) that is required for complementation of a functional GFP. The disclosure also provides non-canonical peptides having cell-penetrating function that differ structurally from known CPPs such as TAT, VP22, transportan and penetratin, and that are capable of translocating cell membranes and escaping the endosome. The disclosed peptides have utility in transporting cargo therapeutics and diagnostics into cells.

摘要翻译： 本公开提供了分离具有细胞穿透功能的肽的方法，其中肽仅在其通过细胞膜的移位后才被检测为生物素化分子。 本公开还提供了用于验证肽的细胞穿透功能的方法，或者可以以其本身的权利来分离这些肽，其中肽可以通过将可检测的货物运输到细胞质中而被检测，例如 作为补充功能性GFP所需的货物毒素或绿色荧光蛋白（GFP）的片段。 本公开还提供了具有细胞穿透功能的非规范肽，其结构不同于已知的CPP，例如TAT，VP22，转运蛋白和穿透素，并且能够移位细胞膜并逃逸内体。 所公开的肽可用于将货物治疗和诊断转运到细胞中。

公开(公告)号：US20150232835A1

公开(公告)日：2015-08-20

申请号：US14580007

申请日：2014-12-22

申请人： Phylogica Limited

发明人： Paul Michael Watt ,  Wayne Robert Thomas ,  Richard Hopkins

IPC分类号： C12N15/10

CPC分类号： C12N15/1082 ,  C07K7/06 ,  C07K7/08 ,  C07K14/001 ,  C12N15/1034 ,  C12N15/1086 ,  C40B30/04 ,  G01N33/569 ,  G01N33/574 ,  Y02A50/57 ,  Y02A50/58

摘要： The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid fragments of the libraries are derived from two or more diverse characterized genomes.

摘要翻译： 本发明提供用于产生表达高度多样化肽或蛋白质结构域的核酸片段文库的新方法，特别是用于产生核酸片段文库的方法，其中文库的核酸片段衍生自两个或多个不同特征的基因组。

公开(公告)号：US20180209960A1

公开(公告)日：2018-07-26

申请号：US15838164

申请日：2017-12-11

申请人： Phylogica Limited

发明人： Paul Michael Watt ,  Richard Hopkins ,  Katrin Hoffman

IPC分类号： G01N33/50 ,  C07K14/47 ,  C40B60/12

CPC分类号： G01N33/5014 ,  C07K14/4723 ,  C07K2319/00 ,  C07K2319/74 ,  C12N2503/02 ,  C40B60/12

摘要： The present invention provides a method of determining or identifying or isolating a cell-penetrating peptide (CPP) or analog or derivative thereof having cell-type selectivity and/or at least capable of passing through a Blood Brain Barrier of an animal subject. This invention also provides CPPs and analogs and derivatives thereof, such as those set forth in SEQ ID NOs: 1-27 of the Sequence Listing, and compositions comprising one or more of the CPPs, including conjugates in which a CPP or analog or derivative thereof is linked to a cargo molecule. The invention also provides methods for transporting cargo molecules across cell membranes to specific locations within cells, and for treating, preventing and/or diagnosing diseases that are treatable by a cargo molecule to which a CPP or analog or derivative of the invention is attached. The invention also provides tailored peptide libraries for use in identifying or isolating CPPs.

公开(公告)号：US10288601B2

公开(公告)日：2019-05-14

申请号：US15838164

申请日：2017-12-11

申请人： Phylogica Limited

发明人： Paul Michael Watt ,  Richard Hopkins ,  Katrin Hoffman

IPC分类号： G01N33/50 ,  C07K14/47 ,  C40B60/12

摘要： The present invention provides a method of determining or identifying or isolating a cell-penetrating peptide (CPP) or analog or derivative thereof having cell-type selectivity and/or at least capable of passing through a Blood Brain Barrier of an animal subject. This invention also provides CPPs and analogs and derivatives thereof, such as those set forth in SEQ ID NOs: 1-27 of the Sequence Listing, and compositions comprising one or more of the CPPs, including conjugates in which a CPP or analog or derivative thereof is linked to a cargo molecule. The invention also provides methods for transporting cargo molecules across cell membranes to specific locations within cells, and for treating, preventing and/or diagnosing diseases that are treatable by a cargo molecule to which a CPP or analog or derivative of the invention is attached. The invention also provides tailored peptide libraries for use in identifying or isolating CPPs.