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223 条记录 (耗时 0.06 秒)

    Lung cancer biomarkers and uses thereof
    4.
    发明授权
    Lung cancer biomarkers and uses thereof 有权

    公开(公告)号：US10359425B2

    公开(公告)日：2019-07-23

    申请号：US12556480

    申请日：2009-09-09

    申请人： Larry Gold ,  Marty Stanton ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A. E. Stewart

    发明人： Larry Gold ,  Marty Stanton ,  Edward N. Brody ,  Rachel M. Ostroff ,  Dominic Zichi ,  Alex A. E. Stewart

    IPC分类号： G01N33/574

    摘要： The present application includes biomarkers, methods, devices, reagents, systems, and kits for the detection and diagnosis of lung cancer. In one aspect, the application provides biomarkers that can be used alone or in various combinations to diagnose lung cancer or permit the differential diagnosis of pulmonary nodules as benign or malignant. In another aspect, methods are provided for diagnosing lung cancer in an individual, where the methods include detecting, in a biological sample from an individual, at least one biomarker value corresponding to at least one biomarker selected from the group of biomarkers provided in Table 1, Col. 2, wherein the individual is classified as having lung cancer, or the likelihood of the individual having lung cancer is determined, based on the at least one biomarker value.

    Method for purification and identification of sperm cells
    5.
    发明授权
    Method for purification and identification of sperm cells 有权
    纯化和鉴定精子细胞的方法

    公开(公告)号：US08703416B2

    公开(公告)日：2014-04-22

    申请号：US13525271

    申请日：2012-06-15

    申请人： Glenn Sanders ,  Evaldas Katilius ,  Larry Gold

    发明人： Glenn Sanders ,  Evaldas Katilius ,  Larry Gold

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12N15/115 ,  C12N5/061 ,  C12N2310/16 ,  C12N2320/10 ,  C12N2330/00

    摘要： The present disclosure describes the isolation, identification and purification of aptamers having sufficiently high affinity and specificity to capture and immobilize intact sperm cells in the presence of female epithelial cells and other non-sperm semen components. The present disclosure also describes affinity-based methods for the detection of sperm cells in samples, including from forensic sample surrogates consisting of swab eluates containing a mixture of HeLa cells and sperm cells. The present disclosure describes methods for eluting sperm cell samples from swabs; methods for purifying sperm cells and methods for amplification and analysis of male DNA. The affinity-based system described herein is inexpensive, simple to use and easily implemented in forensic laboratories.

    摘要翻译： 本公开描述了具有足够高的亲和性和特异性的适体的分离，鉴定和纯化，以在存在雌性上皮细胞和其它非精液精液组分的情况下捕获和固定完整精子细胞。 本公开还描述了用于检测样品中精子细胞的基于亲和性的方法，包括由包含HeLa细胞和精子细胞的混合物的拭子洗脱液组成的法医样品替代物。 本公开描述了从拭子洗脱精子细胞样品的方法; 纯化精子细胞的方法和扩增和分析雄性DNA的方法。 本文描述的基于亲和性的系统是廉价的，易于使用并且容易地在法医实验室中实施。

    Nucleic Acid Ligand Diagnostic Biochip
    9.
    发明申请
    Nucleic Acid Ligand Diagnostic Biochip 审中-公开
    核酸配体诊断生物芯片

    公开(公告)号：US20100209935A1

    公开(公告)日：2010-08-19

    申请号：US12771895

    申请日：2010-04-30

    申请人： Larry Gold ,  Dominic Zichi

    发明人： Larry Gold ,  Dominic Zichi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/00 ,  Y10S977/924 ,  C12Q2565/514 ,  C12Q2565/101 ,  C12Q2541/101 ,  C12Q2525/205

    摘要： A nucleic acid ligand “biochip” is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.

    摘要翻译： 公开了一种核酸配体“生物芯片”，其由以空间限定的方式连接一个或多个特定核酸配体的固体支持物组成。 每个核酸配体特异性地并且强烈地结合到测试混合物（例如体液）中所含的特定靶分子。 靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。 将测试混合物与生物芯片接触导致靶分子与其同源核酸配体的结合。 然后生物芯片可以与与蛋白质共价反应的试剂与不与核酸反应的试剂接触。 然后可以通过检测生物芯片上适当地址处的试剂的存在来检测测试混合物中的每个蛋白质靶标。

    Nucleic acid ligand diagnostic biochip
    10.
    发明授权
    Nucleic acid ligand diagnostic biochip 有权
    核酸配体诊断生物芯片

    公开(公告)号：US07709192B2

    公开(公告)日：2010-05-04

    申请号：US10375487

    申请日：2003-02-27

    申请人： Larry Gold ,  Dominic Zichi

    发明人： Larry Gold ,  Dominic Zichi

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/00 ,  Y10S977/924 ,  C12Q2565/514 ,  C12Q2565/101 ,  C12Q2541/101 ,  C12Q2525/205

    摘要： A nucleic acid ligand “biochip” is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.

    摘要翻译： 公开了一种核酸配体“生物芯片”，其由以空间限定的方式连接一个或多个特定核酸配体的固体支持物组成。 每个核酸配体特异性地并且强烈地结合到测试混合物（例如体液）中所含的特定靶分子。 靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。 将测试混合物与生物芯片接触导致靶分子与其同源核酸配体的结合。 然后生物芯片可以与与蛋白质共价反应的试剂与不与核酸反应的试剂接触。 然后可以通过检测生物芯片上适当地址处的试剂的存在来检测测试混合物中的每个蛋白质靶标。