公开(公告)号：US08784821B1

公开(公告)日：2014-07-22

申请号：US10554731

申请日：2004-05-26

申请人： Peter Kufer ,  Tobias Raum ,  Meera Berry ,  Roman Kischel ,  Susanne Mangold ,  Eva Krinner ,  Birgit Kohleisen ,  Steven Zeman ,  Christian Itin ,  Patrick Bäuerle

发明人： Peter Kufer ,  Tobias Raum ,  Meera Berry ,  Roman Kischel ,  Susanne Mangold ,  Eva Krinner ,  Birgit Kohleisen ,  Steven Zeman ,  Christian Itin ,  Patrick Bäuerle

IPC分类号： A61K38/00

CPC分类号： C07K16/24 ,  A61K2039/505 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2896 ,  C07K16/30 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention provides a method for the preparation of a human binding molecule, fragment or derivative thereof which specifically binds to the human CD3 complex. Furthermore, the invention provides a human binding molecules specifically binding to the human CD3 complex and means comprising said human binding molecules.

摘要翻译： 本发明提供了制备与人CD3复合物特异性结合的人结合分子，其片段或衍生物的方法。 此外，本发明提供了特异性结合人CD3复合物的人结合分子和包含所述人结合分子的手段。

公开(公告)号：US08790645B2

公开(公告)日：2014-07-29

申请号：US13079453

申请日：2011-04-04

申请人： Peter Kufer ,  Meera Berry ,  Sonja Offner ,  Klaus Brischwein ,  Andreas Wolf ,  Tobias Raum ,  Birgit Kohleisen ,  Ulla Lenkkeri-Schütz ,  Patrick Bauerle

发明人： Peter Kufer ,  Meera Berry ,  Sonja Offner ,  Klaus Brischwein ,  Andreas Wolf ,  Tobias Raum ,  Birgit Kohleisen ,  Ulla Lenkkeri-Schütz ,  Patrick Bauerle

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07K16/30 ,  C07K16/28

CPC分类号： C07K16/30 ,  A61K2039/505 ,  C07K16/2809 ,  C07K16/3076 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  Y10S435/81 ,  Y10S435/975

摘要： The present invention provides a pharmaceutical composition comprising a bispecific single chain antibody construct. Said bispecific single chain antibody construct is characterized to comprise or consist of at least two domains, whereby one of said at least two domains specifically binds to human EpCAM and comprises at least one CDR-H3 region comprising the amino acid sequence NXID antigen and a second domain binds to human CD3 antigen. The invention further provides a process for the production of the pharmaceutical composition of the invention, a method for the prevention, treatment or amelioration of a tumorous disease and the use of the disclosed bispecific single chain antibody construct and corresponding means in the prevention, treatment or amelioration of a tumorous disease.

摘要翻译： 本发明提供包含双特异性单链抗体构建体的药物组合物。 所述双特异性单链抗体构建体的特征在于包含至少两个结构域或由至少两个结构域组成，其中所述至少两个结构域之一特异性结合人EpCAM并且包含至少一个CDR-H3区，其包含氨基酸序列NXID抗原和第二 结构域结合人CD3抗原。 本发明进一步提供了制备本发明的药物组合物的方法，一种预防，治疗或改善肿瘤疾病的方法，以及所公开的双特异性单链抗体构建体的用途和相应的手段，用于预防，治疗或 改善肿瘤疾病

公开(公告)号：US20110256137A1

公开(公告)日：2011-10-20

申请号：US13079453

申请日：2011-04-04

申请人： Peter Kufer ,  Meera Berry ,  Sonja Offner ,  Klaus Brischwein ,  Andreas Wolf ,  Tobias Raum ,  Birgit Kohleisen ,  Ulla Lenkkeri-Schutz ,  Patrick Bauerle

发明人： Peter Kufer ,  Meera Berry ,  Sonja Offner ,  Klaus Brischwein ,  Andreas Wolf ,  Tobias Raum ,  Birgit Kohleisen ,  Ulla Lenkkeri-Schutz ,  Patrick Bauerle

IPC分类号： A61K39/395 ,  C07H21/00 ,  A61P35/00 ,  C12P21/02 ,  C12N5/10 ,  C12N1/00 ,  C07K16/28 ,  C12N15/63

CPC分类号： C07K16/30 ,  A61K2039/505 ,  C07K16/2809 ,  C07K16/3076 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  Y10S435/81 ,  Y10S435/975

摘要： The present invention provides a pharmaceutical composition comprising a bispecific single chain antibody construct. Said bispecific single chain antibody construct is characterized to comprise or consist of at least two domains, whereby one of said at least two domains specifically binds to human EpCAM and comprises at least one CDR-H3 region comprising the amino acid sequence NXID antigen and a second domain binds to human CD3 antigen. The invention further provides a process for the production of the pharmaceutical composition of the invention, a method for the prevention, treatment or amelioration of a tumorous disease and the use of the disclosed bispecific single chain antibody construct and corresponding means in the prevention, treatment or amelioration of a tumorous disease.

摘要翻译： 本发明提供包含双特异性单链抗体构建体的药物组合物。 所述双特异性单链抗体构建体的特征在于包含至少两个结构域或由至少两个结构域组成，其中所述至少两个结构域之一特异性结合人EpCAM并且包含至少一个CDR-H3区，其包含氨基酸序列NXID抗原和第二 结构域结合人CD3抗原。 本发明进一步提供了制备本发明的药物组合物的方法，一种预防，治疗或改善肿瘤疾病的方法，以及所公开的双特异性单链抗体构建体的用途和相应的手段，用于预防，治疗或 改善肿瘤疾病

公开(公告)号：US07919089B2

公开(公告)日：2011-04-05

申请号：US10554851

申请日：2004-05-26

申请人： Peter Kufer ,  Meera Berry ,  Sonja Offner ,  Klaus Brischwein ,  Andreas Wolf ,  Tobias Raum ,  Birgit Kohleisen ,  Ulla Lenkkeri-Schutz ,  Patrick Baeuerle

发明人： Peter Kufer ,  Meera Berry ,  Sonja Offner ,  Klaus Brischwein ,  Andreas Wolf ,  Tobias Raum ,  Birgit Kohleisen ,  Ulla Lenkkeri-Schutz ,  Patrick Baeuerle

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07K16/24

CPC分类号： C07K16/30 ,  A61K2039/505 ,  C07K16/2809 ,  C07K16/3076 ,  C07K2317/31 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  Y10S435/81 ,  Y10S435/975

摘要： The present invention provides a pharmaceutical composition comprising a bispecific single chain antibody construct. Said bispecific single chain antibody construct is characterized to comprise or consist of at least two domains, whereby one of said at least two domains specifically binds to human EpCAM and comprises at least one CDR-H3 region comprising the amino acid sequence NXID antigen and a second domain binds to human CD3 antigen. The invention further provides a process for the production of the pharmaceutical composition of the invention, a method for the prevention, treatment or amelioration of a tumorous disease and the use of the disclosed bispecific single chain antibody construct and corresponding means in the prevention, treatment or amelioration of a tumorous disease.

摘要翻译： 本发明提供包含双特异性单链抗体构建体的药物组合物。 所述双特异性单链抗体构建体的特征在于包含至少两个结构域或由至少两个结构域组成，其中所述至少两个结构域之一特异性结合人EpCAM并且包含至少一个CDR-H3区，其包含氨基酸序列NXID抗原和第二 结构域结合人CD3抗原。 本发明进一步提供了制备本发明的药物组合物的方法，一种预防，治疗或改善肿瘤疾病的方法，以及所公开的双特异性单链抗体构建体的用途和相应的手段，用于预防，治疗或 改善肿瘤疾病

公开(公告)号：US20100183615A1

公开(公告)日：2010-07-22

申请号：US12594729

申请日：2008-04-03

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbuese ,  Patrick Hoffmann ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07H21/04 ,  A61P35/00 ,  A61P37/00 ,  C12N15/74 ,  C12N5/10

CPC分类号： C07K16/2809 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/32 ,  C07K16/40 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/626 ,  C07K2317/73 ,  C07K2317/90 ,  C07K2317/92 ,  C07K2319/32 ,  C07K2319/43

摘要： The present invention relates to a polypeptide comprising a first human binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 (epsilon) chain and a second binding domain capable of binding to EGFR, Her2/neu or IgE of a human and/or a non-chimpanzee primate as well as to a process for the production of the mentioned polypeptide. The invention further relates to nucleic acids encoding for the polypeptide, to vectors comprising the same and to host cells comprising the vector. In another aspect, the invention provides for a pharmaceutical composition comprising the mentioned polypeptide and medical uses of the polypeptide.

摘要翻译： 本发明涉及包含能够结合人和非黑猩猩灵长类CD3（ε）链的表位的第一人结合结构域和能够结合人的EGFR，Her2 / neu或IgE的第二结合结构域的多肽 和/或非黑猩猩灵长类动物以及生产所述多肽的方法。 本发明还涉及编码多肽的核酸，包含其的载体和包含载体的宿主细胞。 另一方面，本发明提供包含所述多肽的药物组合物和多肽的医学用途。

公开(公告)号：US10981998B2

公开(公告)日：2021-04-20

申请号：US13122280

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffman ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffman ,  Matthias Klinger ,  Doris Rau ,  Susanne Mangold

IPC分类号： C07K16/28

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain consisting of one antibody variable domain capable of binding to an epitope of the human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to an epitope of a human and a non-chimpanzee primate tumor target antigen. The invention further relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3ϵ (epsilon) chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain consisting of one antibody variable domain capable of binding to an epitope of a human and a non-chimpanzee primate tumor target antigen. The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

公开(公告)号：US09587036B2

公开(公告)日：2017-03-07

申请号：US13638223

申请日：2011-04-01

申请人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carola Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

发明人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carola Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

IPC分类号： C07K16/46 ,  A61K39/395 ,  C07K16/28 ,  C07K16/30

CPC分类号： C07K16/468 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/3069 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20130129730A1

公开(公告)日：2013-05-23

申请号：US13638223

申请日：2011-04-01

申请人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carole Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

发明人： Peter Kufer ,  Ralf Lutterbuese ,  Matthias Klinger ,  Petra Fluhr ,  Doris Rau ,  Susanne Hausmann ,  Carole Steiger ,  Tobias Raum ,  Patrick Hoffmann ,  Roman Kischel ,  Evelyne Schaller ,  Susanne Mangold

IPC分类号： C07K16/46

CPC分类号： C07K16/468 ,  C07K16/2809 ,  C07K16/30 ,  C07K16/3069 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/622

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20120034228A1

公开(公告)日：2012-02-09

申请号：US13122242

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Doris Rau ,  Matthias Klinger ,  Susanne Mangold ,  Claudia Blümel

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lutterbüse ,  Patrick Hoffmann ,  Doris Rau ,  Matthias Klinger ,  Susanne Mangold ,  Claudia Blümel

IPC分类号： A61K39/395 ,  C12N15/62 ,  C12N15/63 ,  C12P21/02 ,  A61P35/02 ,  C12N1/19 ,  C12N5/10 ,  C12N1/15 ,  A61P35/00 ,  C07K16/46 ,  C12N1/21

CPC分类号： C07K16/2809 ,  A61K2039/505 ,  C07K16/22 ,  C07K16/2803 ,  C07K16/2851 ,  C07K16/2863 ,  C07K16/30 ,  C07K16/3069 ,  C07K16/40 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to an antigen selected from the group consisting of Prostate Stem Cell Antigen (PSCA), B-Lymphocyte antigen CD19 (CD19), hepatocyte growth factor receptor (C-MET), Endosialin, the EGF-like domain 1 of EpCAM, encoded by exon 2, Fibroblast activation protein alpha (FAP alpha) and Insulin-like growth factor I receptor (IGF-IR or IGF-1R). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain antibody molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合选自前列腺干细胞抗原（PSCA），B-淋巴细胞抗原CD19（CD19），肝细胞生长因子受体（C- MET），内皮唾液酸蛋白，由外显子2编码的EpCAM的EGF样结构域1，成纤维细胞激活蛋白α（FAPα）和胰岛素样生长因子I受体（IGF-1R或IGF-1R）。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。

公开(公告)号：US20110293619A1

公开(公告)日：2011-12-01

申请号：US13122245

申请日：2009-10-01

申请人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lütterbuse ,  Patrick Hoffmann ,  Doris Rau ,  Susanne Mangold ,  Matthias Klinger ,  Evelyne aschaller ,  Susanne Hausmann ,  Petra Fluhr ,  Carola Steiger

发明人： Peter Kufer ,  Tobias Raum ,  Roman Kischel ,  Ralf Lütterbuse ,  Patrick Hoffmann ,  Doris Rau ,  Susanne Mangold ,  Matthias Klinger ,  Evelyne aschaller ,  Susanne Hausmann ,  Petra Fluhr ,  Carola Steiger

IPC分类号： A61K39/395 ,  C12N5/10 ,  C12N1/00 ,  A61P35/00 ,  C12N15/63 ,  C07K16/18 ,  C07H21/04 ,  C12P21/00 ,  C12N1/21

CPC分类号： C07K16/3069 ,  A61K2039/505 ,  C07K2317/31 ,  C07K2317/34

摘要： The present invention relates to a bispecific single chain antibody molecule comprising a first binding domain capable of binding to an epitope of human and non-chimpanzee primate CD3 epsilon chain, wherein the epitope is part of an amino acid sequence comprised in the group consisting of SEQ ID NOs. 2, 4, 6, and 8, and a second binding domain capable of binding to prostate-specific membrane antigen (PSMA). The invention also provides nucleic acids encoding said bispecific single chain antibody molecule as well as vectors and host cells and a process for its production. The invention further relates to pharmaceutical compositions comprising said bispecific single chain antibody molecule and medical uses of said bispecific single chain anti-body molecule.

摘要翻译： 本发明涉及双特异性单链抗体分子，其包含能够结合人和非黑猩猩灵长类CD3ε链的表位的第一结合结构域，其中所述表位是包含在SEQ ID NO：1中的氨基酸序列的一部分 身份证号 2,4,6和8以及能够结合前列腺特异性膜抗原（PSMA）的第二结合结构域。 本发明还提供了编码所述双特异性单链抗体分子以及载体和宿主细胞的核酸及其生产方法。 本发明还涉及包含所述双特异性单链抗体分子的药物组合物和所述双特异性单链抗体分子的医学应用。