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1.
公开(公告)号:US20090081192A1
公开(公告)日:2009-03-26
申请号:US11860488
申请日:2007-09-24
CPC分类号: G01N33/5064 , G01N33/5088 , G01N2400/50 , G01N2800/328
摘要: A method screens compounds out of the group consisting of proteins, peptides, peptidomimetics, antibodies and small organic molecules that increase the activity of the protein Rac1 by virtue of their binding to the extracellular portion of this protein. The method includes the steps of: contacting a confluent layer of cultured endothelial cells with at least one of test compounds, lysing the endothelial cells with a lysation buffer, and measuring the amount of Rac1 activity.
摘要翻译: 一种方法筛选由蛋白质,肽,肽模拟物,抗体和小有机分子组成的组,其通过与蛋白质的细胞外部分结合而增加蛋白Rac1的活性。 所述方法包括以下步骤:使培养的内皮细胞的汇合层与至少一种测试化合物,裂解内皮细胞与裂解缓冲液和测量Rac1活性的量接触。