公开(公告)号：US20060246079A1

公开(公告)日：2006-11-02

申请号：US11040580

申请日：2005-01-21

申请人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

发明人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

IPC分类号： A61K39/40 ,  C07K16/12

CPC分类号： C07K16/1278 ,  A61K39/40 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/76 ,  C07K2317/92 ,  A61K2300/00

摘要： A highly efficient method for generating human antibodies using recall technology is provided. In one aspect, human antibodies which are specific to the anthrax toxin are provided. In one aspect, human peripheral blood cells that have been pre-exposed to anthrax toxin are used in the SCID mouse model. This method results in high human antibody titers which are primarily of the IgG isotype and which contain antibodies of high specificity and affinity to desired antigens. The antibodies generated by this method can be used therapeutically and prophylactically for preventing or treating mammals exposed to anthrax. Thus, in one embodiment, a prophylactic or therapeutic agent used to counter the effects of anthrax toxin, released as a mechanism of bioterrorism, is provided. In one embodiment, a formulation and method for preventing and/or treating anthrax infection comprising a binding agent that prevents the assembly of the PA63 heptamer is also provided. Methods for diagnosis and methods to determine anthrax contamination are also described.

摘要翻译： 提供了使用召回技术产生人抗体的高效方法。 一方面，提供了对炭疽毒素特异性的人抗体。 一方面，在SCID小鼠模型中使用预先暴露于炭疽毒素的人外周血细胞。 该方法导致高的人抗体滴度，其主要是IgG同种型，并且其含有对所需抗原具有高特异性和亲和力的抗体。 通过该方法产生的抗体可以治疗和预防用于预防或治疗暴露于炭疽的哺乳动物。 因此，在一个实施方案中，提供了用作抵抗作为生物恐怖机制的机制释放的炭疽毒素的作用的预防或治疗剂。 在一个实施方案中，还提供了用于预防和/或治疗炭疽感染的制剂和方法，其包含防止PA63七聚体组装的结合剂。 还描述了诊断方法和确定炭疽污染的方法。

公开(公告)号：US20050281830A1

公开(公告)日：2005-12-22

申请号：US11041471

申请日：2005-01-24

申请人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

发明人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

IPC分类号： A61K39/40 ,  G01N33/554 ,  G01N33/569

CPC分类号： G01N33/6854 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/505 ,  A61K2039/507 ,  C07K16/1278 ,  C07K2317/21 ,  G01N2333/35 ,  A61K2300/00

摘要： A highly efficient method for generating human antibodies using recall technology is provided. In one aspect, human antibodies which are specific to the anthrax toxin are provided. In one aspect, human peripheral blood cells that have been pre-exposed to anthrax toxin are used in the SCID mouse model. This method results in high human antibody titers which are primarily of the IgG isotype and which contain antibodies of high specificity and affinity to desired antigens. The antibodies generated by this method can be used therapeutically and prophylactically for preventing or treating mammals exposed to anthrax. Thus, in one embodiment, a prophylactic or therapeutic agent used to counter the effects of anthrax toxin, released as a mechanism of bioterrorism, is provided. In one embodiment, a formulation and method for preventing and/or treating anthrax infection comprising a binding agent that prevents the assembly of the PA63 heptamer is also provided. Methods for diagnosis and methods to determine anthrax contamination are also described.

摘要翻译： 提供了使用召回技术产生人抗体的高效方法。 一方面，提供了对炭疽毒素特异性的人抗体。 一方面，在SCID小鼠模型中使用预先暴露于炭疽毒素的人外周血细胞。 该方法导致高的人抗体滴度，其主要是IgG同种型，并且其含有对所需抗原具有高特异性和亲和力的抗体。 通过该方法产生的抗体可以治疗和预防用于预防或治疗暴露于炭疽的哺乳动物。 因此，在一个实施方案中，提供了用作抵抗作为生物恐怖机制的机制释放的炭疽毒素的作用的预防或治疗剂。 在一个实施方案中，还提供了用于预防和/或治疗炭疽感染的制剂和方法，其包含防止PA63七聚体组装的结合剂。 还描述了诊断方法和确定炭疽污染的方法。

公开(公告)号：US20080063647A1

公开(公告)日：2008-03-13

申请号：US11041318

申请日：2005-01-24

申请人： Phillip R. Morrow ,  Angray S. Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz ,  Jeanne Morrow

发明人： Phillip R. Morrow ,  Angray S. Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz ,  Jeanne Morrow

IPC分类号： A61K39/40 ,  C07K16/12

CPC分类号： C07K16/1278 ,  A61K39/40 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/76 ,  C07K2317/92 ,  A61K2300/00

摘要： A highly efficient method for generating human antibodies using recall technology is provided. In one aspect, human antibodies which are specific to the anthrax toxin are provided. In one aspect, human peripheral blood cells that have been pre-exposed to anthrax toxin are used in the SCID mouse model. This method results in high human antibody titers which are primarily of the IgG isotype and which contain antibodies of high specificity and affinity to desired antigens. The antibodies generated by this method can be used therapeutically and prophylactically for preventing or treating mammals exposed to anthrax. Thus, in one embodiment, a prophylactic or therapeutic agent used to counter the effects of anthrax toxin, released as a mechanism of bioterrorism, is provided. In one embodiment, a formulation and method for preventing and/or treating anthrax infection comprising a binding agent that prevents the assembly of the PA63 heptamer is also provided. Methods for diagnosis and methods to determine anthrax contamination are also described.

摘要翻译： 提供了使用召回技术产生人抗体的高效方法。 一方面，提供了对炭疽毒素特异性的人抗体。 一方面，在SCID小鼠模型中使用预先暴露于炭疽毒素的人外周血细胞。 该方法导致高的人抗体滴度，其主要是IgG同种型，并且其含有对所需抗原具有高特异性和亲和力的抗体。 通过该方法产生的抗体可以治疗和预防用于预防或治疗暴露于炭疽的哺乳动物。 因此，在一个实施方案中，提供了用作抵抗作为生物恐怖机制的机制释放的炭疽毒素的作用的预防或治疗剂。 在一个实施方案中，还提供了用于预防和/或治疗炭疽感染的制剂和方法，其包含阻止PA 63七聚体组装的结合剂。 还描述了诊断方法和确定炭疽污染的方法。

公开(公告)号：US20090202553A1

公开(公告)日：2009-08-13

申请号：US12230549

申请日：2008-08-29

申请人： Phillip R. Morrow ,  Angray S. Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz ,  Jeanne Morrow

发明人： Phillip R. Morrow ,  Angray S. Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz ,  Jeanne Morrow

IPC分类号： C07K16/12 ,  C12N15/13 ,  C12N15/85 ,  C12N5/10 ,  C12P21/08 ,  A61K39/40 ,  A61P31/04

CPC分类号： C07K16/1278 ,  A61K39/40 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/76 ,  C07K2317/92 ,  A61K2300/00

摘要： A highly efficient method for generating human antibodies using recall technology is provided. In one aspect, human antibodies which are specific to the anthrax toxin are provided. In one aspect, human peripheral blood cells that have been pre-exposed to anthrax toxin are used in the SCID mouse model. This method results in high human antibody titers which are primarily of the IgG isotype and which contain antibodies of high specificity and affinity to desired antigens. The antibodies generated by this method can be used therapeutically and prophylactically for preventing or treating mammals exposed to anthrax. Thus, in one embodiment, a prophylactic or therapeutic agent used to counter the effects of anthrax toxin, released as a mechanism of bioterrorism, is provided. In one embodiment, a formulation and method for preventing and/or treating anthrax infection comprising a binding agent that prevents the assembly of the PA63 heptamer is also provided. Methods for diagnosis and methods to determine anthrax contamination are also described.

摘要翻译： 提供了使用召回技术产生人抗体的高效方法。 一方面，提供了对炭疽毒素特异性的人抗体。 一方面，在SCID小鼠模型中使用预先暴露于炭疽毒素的人外周血细胞。 该方法导致高的人抗体滴度，其主要是IgG同种型，并且其含有对所需抗原具有高特异性和亲和力的抗体。 通过该方法产生的抗体可以治疗和预防用于预防或治疗暴露于炭疽的哺乳动物。 因此，在一个实施方案中，提供了用作抵抗作为生物恐怖机制的机制释放的炭疽毒素的作用的预防或治疗剂。 在一个实施方案中，还提供了用于预防和/或治疗炭疽感染的制剂和方法，其包含防止PA63七聚体组装的结合剂。 还描述了诊断方法和确定炭疽污染的方法。

公开(公告)号：US20080081042A1

公开(公告)日：2008-04-03

申请号：US11041763

申请日：2005-01-24

申请人： Phillip R. Morrow ,  Angray S. Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz ,  Jeanne Morrow

发明人： Phillip R. Morrow ,  Angray S. Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz ,  Jeanne Morrow

IPC分类号： A61K39/40 ,  C07K16/12

CPC分类号： C07K16/1278 ,  A61K39/40 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/76 ,  C07K2317/92 ,  A61K2300/00

摘要： A highly efficient method for generating human antibodies using recall technology is provided. In one aspect, human antibodies which are specific to the anthrax toxin are provided. In one aspect, human peripheral blood cells that have been pre-exposed to anthrax toxin are used in the SCID mouse model. This method results in high human antibody titers which are primarily of the IgG isotype and which contain antibodies of high specificity and affinity to desired antigens. The antibodies generated by this method can be used therapeutically and prophylactically for preventing or treating mammals exposed to anthrax. Thus, in one embodiment, a prophylactic or therapeutic agent used to counter the effects of anthrax toxin, released as a mechanism of bioterrorism, is provided. In one embodiment, a formulation and method for preventing and/or treating anthrax infection comprising a binding agent that prevents the assembly of the PA63 heptamer is also provided. Methods for diagnosis and methods to determine anthrax contamination are also described.

摘要翻译： 提供了使用召回技术产生人抗体的高效方法。 一方面，提供了对炭疽毒素特异性的人抗体。 一方面，在SCID小鼠模型中使用预先暴露于炭疽毒素的人外周血细胞。 该方法导致高的人抗体滴度，其主要是IgG同种型，并且其含有对所需抗原具有高特异性和亲和力的抗体。 通过该方法产生的抗体可以治疗和预防用于预防或治疗暴露于炭疽的哺乳动物。 因此，在一个实施方案中，提供了用作抵抗作为生物恐怖机制的机制释放的炭疽毒素的作用的预防或治疗剂。 在一个实施方案中，还提供了用于预防和/或治疗炭疽感染的制剂和方法，其包含防止PA63七聚体组装的结合剂。 还描述了诊断方法和确定炭疽污染的方法。