公开(公告)号：US6146858A

公开(公告)日：2000-11-14

申请号：US462046

申请日：2000-02-14

申请人： Rainhardt Koch ,  Frank Berendes ,  John Foster ,  Hans-Georg Rast ,  Jurgen Engelhardt ,  Jorg Neubauer ,  Wolfgang Koch ,  Klaus Szablikowski

发明人： Rainhardt Koch ,  Frank Berendes ,  John Foster ,  Hans-Georg Rast ,  Jurgen Engelhardt ,  Jorg Neubauer ,  Wolfgang Koch ,  Klaus Szablikowski

IPC分类号： C08B1/00 ,  C08B11/08 ,  C12P19/14 ,  C12S3/04 ,  C12P19/04 ,  C08B1/06 ,  C12N9/26 ,  C12N9/42 ,  D06M16/00

CPC分类号： C12P19/14 ,  C08B1/00 ,  C08B11/08

摘要： Samples of commercial cellulose having high crystallinity (>80%) and a degree of polymerization of approximately 1500 were pretreated enzymatically under various conditions with commercial endoglucanases, before the chemical conversion to substituted cellulose derivatives was carried out. The enzymatically pretreated cellulose samples exhibited a significantly higher substitution, up to 222% higher, in comparison with control samples which had been treated with buffer without enzyme. The increase in substitution during the chemical reaction could be observed in the presence of various amounts of alkali, but fell as the amounts of alkali decreased. At the same degree of substitution of the cellulose derivative, the use of cellulose pretreated with endoglucanase significantly reduced the amount of alkali required by 60%, as compared with the use of cellulose pretreated only with buffer. Furthermore, by reducing the amount of water used in the reaction mixture in the chemical reaction, it was possible further to increase the substitution of enzymatically pretreated cellulose.

摘要翻译： PCT No.PCT / EP98 / 03907 Sec。 371日期2000年2月14日 102（e）日期2000年2月14日PCT提交1998年6月26日PCT公布。 出版物WO99 /​​ 02568 日期1999年1月21日在进行取代纤维素衍生物的化学转化之前，将具有高结晶度（> 80％）和聚合度约1500的市售纤维素样品在各种条件下用商业内切葡聚糖酶预处理。 与没有酶的缓冲液处理的对照样品相比，酶法预处理的纤维素样品显示出高得多的222％的替代。 化学反应中取代的增加可以在各种碱存在下观察到，但随着碱量的降低而降低。 与纤维素衍生物相同的取代度，与仅使用缓冲液预处理的纤维素的使用相比，使用用内切葡聚糖酶预处理的纤维素显着地减少了所需碱量的60％。 此外，通过减少化学反应中反应混合物中使用的水量，可以进一步增加酶预处理的纤维素的取代。