公开(公告)号：US20060057689A1

公开(公告)日：2006-03-16

申请号：US10513812

申请日：2003-04-29

申请人： Ralf Otto ,  Georg Fieg ,  Sabine Both ,  Ulrich Schoerken ,  Levent Yueksel ,  Ingomar Mrozek ,  Carolin Meyer ,  Norbert Klein ,  Albrecht Weiss

发明人： Ralf Otto ,  Georg Fieg ,  Sabine Both ,  Ulrich Schoerken ,  Levent Yueksel ,  Ingomar Mrozek ,  Carolin Meyer ,  Norbert Klein ,  Albrecht Weiss

IPC分类号： C12P7/64 ,  C12P7/40

CPC分类号： C12P7/6418 ,  C12P7/40

摘要： Processes for preparing fatty acids are described wherein a C4-C12 fatty acid methyl ester is subjected to hydrolysis in the presence of an enzyme to form an organic phase comprising a C4-C12 fatty acid and an aqueous phase comprising methanol, wherein at least a portion of the methanol is continuously removed; and the organic phase is subsequently separated from the aqueous phase, and optionally, where the organic phase further comprises an unhydrolyzed portion of the C4-C12 fatty acid methyl ester, the unhydrolyzed portion of the C4-C12 fatty acid methyl ester is separated from the C4-C12 fatty acid.

摘要翻译： 描述了制备脂肪酸的方法，其中在酶的存在下使C 4 -C 12脂肪酸甲酯进行水解以形成包含C 脂肪酸和包含甲醇的水相，其中至少部分甲醇被连续除去; 随后将有机相与水相分离，任选地，当有机相进一步包含C 6-14脂肪酸甲酯的未水解部分时， 将C 4 -C 12脂肪酸甲酯的未水解部分与C 4 -C 12脂肪酸甲酯分离 >脂肪酸。

公开(公告)号：US06750332B1

公开(公告)日：2004-06-15

申请号：US09856835

申请日：2002-02-26

申请人： Ralf Otto ,  Albrecht Weiss

发明人： Ralf Otto ,  Albrecht Weiss

IPC分类号： C07H1500

CPC分类号： C07H15/04 ,  A61K8/602 ,  A61Q19/00 ,  C08B37/00 ,  C12P7/62 ,  C12P19/44

摘要： Novel salicyl alcohol derivatives having valuable cosmetically and pharmaceutically useful properties, such as prostaglandin synthesis inhibition, corresponding to the formula (I): R1—OCH2—Ph—O—Z—(R2)n  (I), a method for producing the same and their utilization in cosmetics and pharmacy.

摘要翻译： 新颖的水杨醇衍生物具有有价值的美容和药学上有用的性质，如前列腺素合成抑制，对应于式（I）：其制备方法及其在化妆品和药学中的应用。

公开(公告)号：US20080176811A1

公开(公告)日：2008-07-24

申请号：US12057425

申请日：2008-03-28

申请人： Bernadette Geers ,  Ralf Otto ,  Albrecht Weiss ,  Dirk Petersohn ,  Klaus Rudolf Schroeder ,  Kordula Schlotmann

发明人： Bernadette Geers ,  Ralf Otto ,  Albrecht Weiss ,  Dirk Petersohn ,  Klaus Rudolf Schroeder ,  Kordula Schlotmann

IPC分类号： A61K31/7048 ,  C07H17/06 ,  C12P19/60 ,  A61P17/00 ,  A61Q19/02

CPC分类号： A61K8/602 ,  A23L33/105 ,  A23V2002/00 ,  A61K8/73 ,  A61Q19/02 ,  C07H17/07 ,  C12P7/62 ,  C12P17/06 ,  C12P19/60 ,  A23V2250/2116 ,  A23V2250/1866

摘要： Substituted and unsubstituted flavone or isoflavone glycoside derivatives of the formula [A1-C(═O)O]m—[X—O-Z]—[O—C(═O)-A2]n, wherein [X—O-Z] represents a flavone or isoflavone glycoside structure, particularly a naringin residue, X is a flavone or isoflavone corresponding to formula (IIa) or formula (IIb): wherein the flavone or isoflavone residue is substituted one or more times and/or reduced one or more times; Z represents a mono-, di- or polysaccharide, which is acetally-bound at the benzopyran group to X and ester-substituted by —O—C(═O)-A2; [A1-C(═O)] is an acyl group on the flavone or isoflavone; A1 and A2 independently, represent a polyunsaturated C15-26 alkenyl group containing at least four isolated and/or at least two conjugated double bonds, or an arylaliphatic radical with 1-to-4 methylene groups between the ester group and the aromatic ring; [C(═O)A2] is an acyl group; n is an integer other than 0; m is an integer, including 0; and R1, R2 and R are hydroxyl groups or hydrogen atoms.

摘要翻译： 取代和未取代的式[A-1-C（-O）O] - [XOZ] - [OC（-O）-A）的黄酮或异黄酮糖苷衍生物 其中[XOZ]表示黄酮或异黄酮糖苷结构，特别是柚皮苷残基，X是与式（IIa）或式（IIa）相应的黄酮或异黄酮 IIb）：其中所述黄酮或异黄酮残基被一次或多次取代和/或还原一次或多次; Z表示一种，二或多糖，其在苯并吡喃基团处与X缩合而X和被-O-C（-O）-A 2 - 被酯取代。 [A-1] -C（-O）]是黄酮或异黄酮上的酰基; A 1和A 2独立地表示含有至少四个分离和/或至少两个共轭双键的多不饱和C 15-26亚烯基 或在酯基和芳香环之间具有1至4个亚甲基的芳基脂族基; [C（-O）A 2]是酰基; n是0以外的整数; m是整数，包括0; 并且R 1，R 2和R 2是羟基或氢原子。

公开(公告)号：US06897328B2

公开(公告)日：2005-05-24

申请号：US10301333

申请日：2002-11-21

申请人： Bernhard Gutsche ,  Albrecht Weiss ,  Ralf Otto ,  Tycho Michel

发明人： Bernhard Gutsche ,  Albrecht Weiss ,  Ralf Otto ,  Tycho Michel

IPC分类号： C11B3/00 ,  C11C3/00 ,  C07C51/43

CPC分类号： C11C3/003 ,  C11B3/003

摘要： A single stage process for deacidifying glycerides involving: (a) providing a glyceride having an acid value of from about 5 to 20; (b) providing a lower alcohol; (c) providing an enzyme catalyst immobilized on a support material; and passing a mixture of (a) and (b), over (c), thereby deacidifying the glycerides.

摘要翻译： 用于脱酸甘油酯的单阶段方法，涉及：（a）提供酸值为约5至20的甘油酯; （b）提供低级醇; （c）提供固定在载体材料上的酶催化剂; 并通过（a）和（b）的混合物，超过（c），从而使甘油酯脱酸。

公开(公告)号：US07833787B2

公开(公告)日：2010-11-16

申请号：US10922577

申请日：2004-08-19

申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

IPC分类号： C12N5/07 ,  C12N5/10 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

公开(公告)号：US20090162900A1

公开(公告)日：2009-06-25

申请号：US12031865

申请日：2008-02-15

申请人： Barbara ENENKEL ,  Juergen Fieder ,  Ralf Otto ,  Stefanos Grammatikos

发明人： Barbara ENENKEL ,  Juergen Fieder ,  Ralf Otto ,  Stefanos Grammatikos

IPC分类号： C12P21/00 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N15/65 ,  C12N15/67 ,  C12N15/69 ,  C12N15/79

摘要： An expression vector for eukaryotic cells comprising a gene which codes for a protein of interest, functionally linked to a hamster-ubiquitin/S27a-promoter and a gene which codes for a fluorescent protein. Preferably the expression vector also contains an amplifiable selectable marker gene.The invention also describes host cells, preferably mammalian cells, which have been transfected with the expression vector, processes for producing heterologous gene products and a method of selecting high-producing cells.

摘要翻译： 用于真核细胞的表达载体，其包含编码与仓鼠 - 泛素/ S27a启动子功能相关的感兴趣的蛋白质的基因和编码荧光蛋白的基因。 优选地，表达载体还含有可扩增选择标记基因。 本发明还描述了已经用表达载体转染的宿主细胞，优选哺乳动物细胞，用于产生异源基因产物的方法和选择高产细胞的方法。

公开(公告)号：US20060121444A1

公开(公告)日：2006-06-08

申请号：US11270236

申请日：2005-11-09

申请人： Hitto Kaufmann ,  Juergen Fieder ,  Ralf Otto

发明人： Hitto Kaufmann ,  Juergen Fieder ,  Ralf Otto

IPC分类号： C12Q1/00 ,  C12N5/06

CPC分类号： G01N33/56966 ,  G01N2333/4718

摘要： Production of biopharmaceuticals from CHO cells requires generation of master-, working- and post-production cell banks of high quality, partly under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to the desire to establish production processes that are completely devoid of serum or even any animal components and to ensure robust thaw performance for reliable production. Here we describe a novel strategy employing flow cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of CHO cell banks. Our data show that this method enables evaluation of a cryopreservation procedure just 6 h after thaw.

公开(公告)号：US06265225B1

公开(公告)日：2001-07-24

申请号：US09172852

申请日：1998-10-15

申请人： Ralf Otto ,  Dieter Bickoni ,  Hugo Wilmes

发明人： Ralf Otto ,  Dieter Bickoni ,  Hugo Wilmes

IPC分类号： G01N3500

CPC分类号： B01L3/50825 ,  B65D45/02 ,  G01N35/1002 ,  G01N2035/0405 ,  Y10T436/2575

摘要： A description is given of a cap for a reagent container having a sealable lid, where the lid can be pivoted laterally upward from the cap sealing position, with the container being opened, by means of an inclined bistable hinge, bears one or more catches which can come into contact with an apparatus for opening or closing the lid, and the cap has one or more centering elements by means of which the reagent-container setting position in the analyzer is fixed.

摘要翻译： 给出了具有可密封盖的试剂容器的盖子的描述，其中盖子可以通过倾斜的双稳态铰链从盖密封位置横向向上枢转，容器被打开，承载一个或多个可以 与用于打开或关闭盖的装置接触，并且盖具有一个或多个定心元件，通过该定心元件固定分析器中的试剂容器设置位置。

公开(公告)号：US08338179B2

公开(公告)日：2012-12-25

申请号：US11969644

申请日：2008-01-04

申请人： Barbara Enenkel ,  Juergen Fieder ,  Ralf Otto ,  Kerstin Sautter ,  Klaus Bergemann

发明人： Barbara Enenkel ,  Juergen Fieder ,  Ralf Otto ,  Kerstin Sautter ,  Klaus Bergemann

IPC分类号： C12N9/12 ,  C12N15/63 ,  C12N5/00 ,  C07H21/04

CPC分类号： C12N9/1205 ,  C12Y207/01095

摘要： The invention relates to modified neomycin phosphotransferase genes and their use in a selection method for high-producing recombinant cells. The invention further relates to expression vectors which contain a modified neomycin phosphotransferase gene and a gene of interest functionally linked to a heterologous promoter and a method of preparing heterologous gene products using these expression vectors.

摘要翻译： 本发明涉及修饰的新霉素磷酸转移酶基因及其在高产重组细胞筛选方法中的应用。 本发明还涉及含有修饰的新霉素磷酸转移酶基因和与异源启动子功能连接的目的基因的表达载体和使用这些表达载体制备异源基因产物的方法。

公开(公告)号：US07569339B2

公开(公告)日：2009-08-04

申请号：US11270236

申请日：2005-11-09

申请人： Hitto Kaufmann ,  Juergen Fieder ,  Ralf Otto

发明人： Hitto Kaufmann ,  Juergen Fieder ,  Ralf Otto

IPC分类号： C12Q1/00 ,  G01N33/48 ,  C12N5/02

CPC分类号： G01N33/56966 ,  G01N2333/4718

摘要： Production of biopharmaceuticals from CHO cells requires generation of master-, working- and post-production cell banks of high quality, partly under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to the desire to establish production processes that are completely devoid of serum or even any animal components and to ensure robust thaw performance for reliable production. Here we describe a novel strategy employing flow cytometric (FC) analysis of Annexin V-stained cells for high-throughput characterization of CHO cell banks. Our data show that this method enables evaluation of a cryopreservation procedure just 6 h after thaw.

摘要翻译： 从CHO细胞生产生物药物需要生产高质量的母体，工作和后期生产细胞库，部分是在GMP条件下生产。 每个新的生产细胞系都需要最佳的冷冻保存策略，特别是关于建立完全没有血清或甚至任何动物成分的生产过程的愿望，并确保可靠生产的强大的解冻性能。 在这里，我们描述了一种采用流式细胞术（FC）分析Annexin V染色细胞用于CHO细胞库的高通量表征的新策略。 我们的数据显示，这种方法能够在融化6小时后进行冷冻保存程序的评估。