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公开(公告)号：US07053282B1
公开(公告)日：2006-05-30
申请号：US09020716
申请日：1998-02-09
申请人： Rudolf Jung , Larry R. Beach , Virginia M. Dress , A. Gururaj Rao , Jerome P. Ranch , David S. Ertl , Regina K. Higgins
发明人： Rudolf Jung , Larry R. Beach , Virginia M. Dress , A. Gururaj Rao , Jerome P. Ranch , David S. Ertl , Regina K. Higgins
CPC分类号： C07K14/811 , C07K14/415 , C12N15/8234 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： The present invention provides a plant seed the endosperm of which is characterized as having an elevated level of a preselected amino acid. The present invention also provides expression cassettes, vectors, plants, plant cells and a method for enhancing the nutritional value of seeds.
摘要翻译： 本发明提供一种植物种子，其胚乳的特征在于具有升高的预选氨基酸水平。 本发明还提供了表达盒，载体，植物，植物细胞和增强种子营养价值的方法。
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2.发明申请Methods of Transforming Plants and Identifying Parental Origin of a Chromosome In Those Plants 审中-公开在这些植物中转化植物和识别染色体的亲本起源的方法公开(公告)号：US20080131896A1
公开(公告)日：2008-06-05
申请号：US11941106
申请日：2007-11-16
申请人： Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
发明人： Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
IPC分类号： C12Q1/68
CPC分类号： C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
摘要翻译： 提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。 所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。 从混合植物获得的组织是转化能力。 然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。 可以选择转化的细胞，并再生转基因杂交植物。 感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。 另外提供转化的植物和种子。
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公开(公告)号：US07057089B2
公开(公告)日：2006-06-06
申请号：US09993080
申请日：2001-11-13
申请人： Jerome P. Ranch , Wallace A. Marsh
发明人： Jerome P. Ranch , Wallace A. Marsh
CPC分类号： A01H4/001
摘要： Methods are provided for transforming freshly isolated, immature maize embryos and for producing transgenic maize plants. The methods comprise obtaining immature embryos from a maize plant, contacting the embryos with an auxin-depleted or phytohormone-depleted transformation support medium and introducing a nucleotide construct into cells from the embryos prior to subjecting the embryos to conditions which promote embryogenic-tissue formation. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.
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4.发明授权公开(公告)号：US07022894B2
公开(公告)日：2006-04-04
申请号：US10784418
申请日：2004-02-23
CPC分类号： C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
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5.发明授权Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs 有权受精卵，卵或精子细胞的转化以及从分离的胚囊恢复转化的植物公开(公告)号：US06300543B1
公开(公告)日：2001-10-09
申请号：US09147489
申请日：1999-05-12
申请人： David D. Cass , Locksley E. McGann , Guichang Zhang , John D. Laurie , Jerome P. Ranch , William J. Gordon-Kamm
发明人： David D. Cass , Locksley E. McGann , Guichang Zhang , John D. Laurie , Jerome P. Ranch , William J. Gordon-Kamm
IPC分类号： C12N1582
CPC分类号： C12N15/8201
摘要： Methodology is provided for the production of uniformly transformed plants capable of transmitting a foreign gene to progeny by sexual reproduction. A foreign gene is introduced into the zygote in an isolated embryo sac and a transformed plant is recovered. Alternatively, a foreign gene is introduced into an egg cell in an isolated embryo sac, the egg cell is fertilized with an isolated sperm cell and a transformed plant is recovered. Sperm cells may be transformed with a foreign gene, an egg cell in an isolated embryo sac is fertilized with the transformed sperm cells, or nuclei isolated from the transformed sperm cells, and a transgenic plant is recovered. Another method for the production of transgenic plants is transformation of an embryo in an isolated embryo sac. The transgenic plant produced by any one of these methods is homogeneously transformed and capable of transmitting the foreign gene to progeny by sexual reproduction.
摘要翻译： 提供方法用于生产能够通过有性繁殖将外来基因传播给后代的均匀转化植物。 将外源基因导入分离的胚囊中的受精卵并回收转化的植物。 或者，外源基因被引入到分离的胚囊中的卵细胞中，卵细胞用分离的精子细胞受精并回收转化的植物。 精子细胞可以用外源基因转化，分离的胚囊中的卵细胞用转化的精子细胞受精，或从转化的精子细胞中分离出核，并回收转基因植物。 用于生产转基因植物的另一种方法是在分离的胚囊中转化胚胎。 通过这些方法中的任何一种产生的转基因植物被均匀转化，并且能够通过有性繁殖将外源基因转移到后代。
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公开(公告)号：US08334429B2
公开(公告)日：2012-12-18
申请号：US12650106
申请日：2009-12-30
CPC分类号： C12N15/8205 , C12N9/1007
摘要： Auxotrophic Agrobacterium and methods employing auxotrophic Agrobacterium are provided. Auxotrophic Agrobacterium may be used in a variety of methods including biologically containing Agrobacterium comprising a transgene and transforming a plant cell without using an Agrobacterium counter-selective agent. Transforming maize immature embryos using an Agrobacterium auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic Agrobacterium. Methods for producing an Agrobacterium thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.
摘要翻译： 营养缺陷型农杆菌和使用营养缺陷型农杆菌的方法。 营养不良农杆菌可以以多种方法使用，包括生物地含有转基因的农杆菌，并且不使用农杆菌反选择剂来转化植物细胞。 使用农杆菌营养缺陷型用于胸苷转化玉米未成熟胚，导致与使用原养性农杆菌的转化相当的转化效率。 公开了用于生产农杆菌营养缺陷型农杆菌和在转化方法中使用营养缺陷型的方法。 还提供了使用本发明的方法生产的转化的组织和植物。
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公开(公告)号：US5008200A
公开(公告)日：1991-04-16
申请号：US197355
申请日：1988-05-23
申请人： Jerome P. Ranch , Julie A. Buchheim
发明人： Jerome P. Ranch , Julie A. Buchheim
CPC分类号： C12N5/0025 , C12N5/04
摘要： Seeds of Glycine max, Glycine soja and hybrids thereof are produced by propagating embryogenic tissue containing multiple immature somatic embryos directly or serially by culturing particular cotyledonary tissue or derivation product thereof on media containing auxin selected from the group consisting of chloro-substituted phenoxyacetic acid, methyl derivative thereof, dicamba or picloram, incubating individual immature somatic embryos or embryogenic tissue containing such in a maturation stage to produce mature somatic embryos, germinating the mature somatic embryos to cause shoot tip formation, cultivating the germinated embryos to provide plantlets with 2 to 5 nodes and roots, cultivating the plantlets to provide whole fertile plants which flower and bear seeds, and recovering the seeds. Culturing to provide immature somatic embryos is carried out utilizing auxin in a concentration within the range of 2.5 to 1000 ppm effective to foster normal development of immature somatic embryos. 2,4-dichlorophenoxyacetic acid is the preferred auxin. Maturation is carried out in B5 or MS medium containing indolebutyric acid and abscissic acid or activated charcoal and sucrose, and germination is carried out in B5 or MS medium containing indolebutyric acid and gibberellic acid or MS medium containing filter sterilized L-proline and sucrose. Plantlet formation is carried out in B5 or MS medium without growth regulators. Plant and seed formation is carried out in soil or potting medium.
摘要翻译： 通过在含有生长素的培养基上培养特定子叶组织或其衍生产物，通过培养含有多个未成熟体细胞胚的胚发生组织来产生大豆，大豆和其杂种的种子，所述培养基含有选自氯取代的苯氧基乙酸，甲基 衍生物，麦草畏或pic lor am am am am，，，nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes nodes 和根，培育小植物以提供花和种子的全部可育植物，并回收种子。 使用浓度在2.5至1000ppm范围内的生长素进行培养以提供未成熟体细胞胚，有效促进未成熟体细胞胚的正常发育。 2,4-二氯苯氧基乙酸是优选的生长素。 在含有吲哚丁酸和离子酸或活性炭和蔗糖的B5或MS培养基中进行成熟，并且在含有吲哚丁酸和赤霉酸或含有过滤灭菌的L-脯氨酸和蔗糖的MS培养基的B5或MS培养基中进行发芽。 在没有生长调节剂的B5或MS培养基中进行小植株形成。 植物和种子形成在土壤或盆栽培养基中进行。
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