-
公开(公告)号：US10422799B2
公开(公告)日：2019-09-24
申请号：US15847677
申请日：2017-12-19
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/558 , G01N33/543 , G01N33/53 , G01N33/50 , G01N33/553
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
-
公开(公告)号：US10416156B2
公开(公告)日：2019-09-17
申请号：US15363895
申请日：2016-11-29
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/558 , G01N33/543 , G01N33/53 , G01N33/50 , G01N33/553
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
-
公开(公告)号：US10422798B2
公开(公告)日：2019-09-24
申请号：US15536621
申请日：2014-12-16
发明人： Keigo Kohno , Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Marie Asami
IPC分类号： G01N33/543 , G01N33/558
摘要： A problem of the present invention is to provide an immunochromatographic test strip avoiding agglutination of colloidal gold while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a hemagglutinating agent and the colloidal gold conjugates as a detection reagent, and to provide immunochromatography using the test strip. To solve the problem, the present inventors reviewed the composition of the existing reagent itself from a completely different viewpoint rather than the selection of type or amount of polyanions, and as a result of extensive study on each element, the inventors surprisingly found that agglutination of colloidal gold may be suppressed by using a particular additive without neutralization by polyanions.
-
公开(公告)号：US10422790B2
公开(公告)日：2019-09-24
申请号：US15853503
申请日：2017-12-22
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/558 , G01N33/53 , G01N33/543 , G01N33/50
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventors reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventors surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
-
公开(公告)号：US10151747B2
公开(公告)日：2018-12-11
申请号：US15389015
申请日：2016-12-22
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/558 , G01N33/53 , G01N33/543 , G01N33/50
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
-
公开(公告)号：US09885707B2
公开(公告)日：2018-02-06
申请号：US14389180
申请日：2013-04-01
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/558 , G01N33/53 , G01N33/543
CPC分类号： G01N33/5306 , B01L2300/0825 , G01N33/5005 , G01N33/54366 , G01N33/54386 , G01N33/54393 , G01N33/558
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
-
公开(公告)号：US20150086974A1
公开(公告)日：2015-03-26
申请号：US14389180
申请日：2013-04-01
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/5306 , B01L2300/0825 , G01N33/5005 , G01N33/54366 , G01N33/54386 , G01N33/54393 , G01N33/558
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
-
8.发明申请IMMUNOCHROMATOGRAPHIC TEST STRIP AND DETECTION METHOD USING IMMUNOCHROMATOGRAPHY FOR DETECTING TARGET IN RED BLOOD CELL-CONTAINING SAMPLE 有权使用免疫荧光法检测红血球细胞样品中的目标的免疫色谱测试条带和检测方法公开(公告)号：US20150064692A1
公开(公告)日：2015-03-05
申请号：US14389222
申请日：2013-04-01
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/558 , G01N33/53
CPC分类号： G01N33/54393 , B01L2300/0825 , G01N33/5002 , G01N33/5306 , G01N33/54346 , G01N33/553 , G01N33/558
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
摘要翻译： 本发明要解决的问题是提供一种免疫色谱测试条和使用免疫色谱法的检测方法，其避免了胶体金共轭物的聚集，同时在使用聚凝胺作为全血的情况下，将全血中的红细胞凝集，然后分离和除去 血凝集剂和胶体金缀合物作为检测试剂。 为了解决这个问题，本发明人从完全不同的观点来看待过去的试剂配置本身，而不是选择聚阴离子的种类和数量，并且作为对每个元素的广泛研究的结果，发明人惊奇地发现胶体金的聚集可以是 通过使用某种缓冲溶液而不使用聚阴离子中和来抑制。
-
公开(公告)号：US11275085B2
公开(公告)日：2022-03-15
申请号：US16539587
申请日：2019-08-13
发明人： Keigo Kohno , Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Marie Asami
IPC分类号： G01N33/543 , G01N33/558
摘要： A problem of the present invention is to provide an immunochromatographic test strip avoiding agglutination of colloidal gold while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a hemagglutinating agent and the colloidal gold conjugates as a detection reagent, and to provide immunochromatography using the test strip. To solve the problem, the present inventors reviewed the composition of the existing reagent itself from a completely different viewpoint rather than the selection of type or amount of polyanions, and as a result of extensive study on each element, the inventors surprisingly found that agglutination of colloidal gold may be suppressed by using a particular additive without neutralization by polyanions.
-
公开(公告)号：US20180120303A1
公开(公告)日：2018-05-03
申请号：US15853503
申请日：2017-12-22
发明人： Mayumi Yoshida , Mitsuaki Yamamoto , Shinya Yokokawa , Tomo Shimizu , Keigo Kono
IPC分类号： G01N33/53 , G01N33/558 , G01N33/543 , G01N33/50
摘要： A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.
-
-
-
-
-
-
-
-
-
搜索结果
13 条记录 (耗时 0.015 秒)