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1.发明授权公开(公告)号：US07745203B2
公开(公告)日：2010-06-29
申请号：US11045646
申请日：2005-01-31
申请人： Sadato Hongo , Minoru Ishikawa , Takiji Ishimura , Shigeru Wakayama , Shinji Yanaga , Kenji Oki , Jun Okada
发明人： Sadato Hongo , Minoru Ishikawa , Takiji Ishimura , Shigeru Wakayama , Shinji Yanaga , Kenji Oki , Jun Okada
CPC分类号： G01N33/5438
摘要： A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.
摘要翻译： 碱基序列检测装置具有形成在基序列检测芯片上的信道。 沿通道形成工作电极，并且包括固定在其上的探针，在通道的内表面上形成相对电极，并且在通道的内表面上形成参考电极。 引入端口从通道的上游侧引入溶液或空气，输送口将溶液或空气输送到通道中，并且样品通过样品注入口注入通道。
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2.发明申请Base sequence detection apparatus and base sequence automatic analyzing apparatus 审中-公开基序检测装置和基序自动分析装置公开(公告)号：US20080060940A1
公开(公告)日：2008-03-13
申请号：US11980409
申请日：2007-10-31
申请人： Sadato Hongo , Minoru Ishikawa , Takiji Ishimura , Shigeru Wakayama , Shinji Yanaga , Kenji Oki , Jun Okada
发明人： Sadato Hongo , Minoru Ishikawa , Takiji Ishimura , Shigeru Wakayama , Shinji Yanaga , Kenji Oki , Jun Okada
IPC分类号： G01N27/416
CPC分类号： G01N33/5438
摘要： A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.
摘要翻译： 碱基序列检测装置具有形成在基序列检测芯片上的信道。 沿通道形成工作电极，并且包括固定在其上的探针，在通道的内表面上形成相对电极，并且在通道的内表面上形成参考电极。 引入端口从通道的上游侧引入溶液或空气，输送口将溶液或空气输送到通道中，并且样品通过样品注入口注入通道。
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3.发明申请公开(公告)号：US20050158787A1
公开(公告)日：2005-07-21
申请号：US11045646
申请日：2005-01-31
申请人： Sadato Hongo , Minoru Ishikawa , Takiji Ishimura , Shigeru Wakayama , Shinji Yanaga , Kenji Oki , Jun Okada
发明人： Sadato Hongo , Minoru Ishikawa , Takiji Ishimura , Shigeru Wakayama , Shinji Yanaga , Kenji Oki , Jun Okada
IPC分类号： C12M1/34 , C12Q1/68 , G01N33/543
CPC分类号： G01N33/5438
摘要： A base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.
摘要翻译： 碱基序列检测装置具有形成在基序列检测芯片上的信道。 沿通道形成工作电极，并且包括固定在其上的探针，在通道的内表面上形成相对电极，并且在通道的内表面上形成参考电极。 引入端口从通道的上游侧引入溶液或空气，输送口将溶液或空气输送到通道中，并且样品通过样品注入口注入通道。
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4.发明授权Method and an apparatus for determining nucleotide sequence, and a computer program product to be executed by the apparatus 有权用于确定核苷酸序列的方法和装置，以及由该装置执行的计算机程序产品公开(公告)号：US08150635B2
公开(公告)日：2012-04-03
申请号：US12949411
申请日：2010-11-18
申请人： Sadato Hongo , Shinji Yanaga
发明人： Sadato Hongo , Shinji Yanaga
CPC分类号： G01N27/3275
摘要： A method for determining nucleotide sequence encompasses: injecting a solution containing a sample DNA into a chip cartridge provided with a detecting electrode, to which a probe DNA is immobilized; introducing an intercalator solution in the chip cartridge; obtaining a current-voltage characteristic curve by measuring a current in the solution due to an electrochemical reaction of the intercalator through the detecting electrode; obtaining a baseline by linearly approximating the current-voltage characteristic curve; obtaining a net current value by subtracting, from a peak current value of the current-voltage characteristic curve, a baseline current value obtained from the baseline at a peak voltage value defining the peak current value; and identifying a nucleotide sequence in the sample DNA, using the net current value.
摘要翻译： 确定核苷酸序列的方法包括：将含有样本DNA的溶液注入设置有检测电极的芯片盒中，固定有探针DNA; 在芯片盒中引入嵌入剂溶液; 通过测量由于嵌入剂通过检测电极的电化学反应而导致的溶液中的电流来获得电流 - 电压特性曲线; 通过线性近似电流 - 电压特性曲线获得基线; 通过从当前电压特性曲线的峰值电流值中减去在定义峰值电流值的峰值电压值处从基线获得的基线电流值来获得净电流值; 并使用净电流值鉴定样品DNA中的核苷酸序列。
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5.发明申请Method and an apparatus for determining nucleotide sequence, and a computer program product to be executed by the apparatus 审中-公开用于确定核苷酸序列的方法和装置，以及由该装置执行的计算机程序产品公开(公告)号：US20060252067A1
公开(公告)日：2006-11-09
申请号：US11377265
申请日：2006-03-17
申请人： Sadato Hongo , Shinji Yanaga
发明人： Sadato Hongo , Shinji Yanaga
CPC分类号： G01N27/3275
摘要： A method for determining nucleotide sequence encompasses: injecting a solution containing a sample DNA into a chip cartridge provided with a detecting electrode, to which a probe DNA is immobilized; introducing an intercalator solution in the chip cartridge; obtaining a current-voltage characteristic curve by measuring a current in the solution due to an electrochemical reaction of the intercalator through the detecting electrode; obtaining a baseline by linearly approximating the current-voltage characteristic curve; obtaining a net current value by subtracting, from a peak current value of the current-voltage characteristic curve, a baseline current value obtained from the baseline at a peak voltage value defining the peak current value; and identifying a nucleotide sequence in the sample DNA, using the net current value.
摘要翻译： 确定核苷酸序列的方法包括：将含有样本DNA的溶液注入设置有检测电极的芯片盒中，固定有探针DNA; 在芯片盒中引入嵌入剂溶液; 通过测量由于嵌入剂通过检测电极的电化学反应而导致的溶液中的电流来获得电流 - 电压特性曲线; 通过线性近似电流 - 电压特性曲线获得基线; 通过从当前电压特性曲线的峰值电流值中减去在定义峰值电流值的峰值电压值处从基线获得的基线电流值来获得净电流值; 并使用净电流值鉴定样品DNA中的核苷酸序列。
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