公开(公告)号：US20110191877A1

公开(公告)日：2011-08-04

申请号：US13011666

申请日：2011-01-21

申请人： Sean M. Russell ,  Joseph F. Petolino

发明人： Sean M. Russell ,  Joseph F. Petolino

IPC分类号： A01H1/00 ,  A01H1/06 ,  A01H5/00 ,  C07H21/00 ,  C12N5/10

CPC分类号： C12N15/8213 ,  A01H1/02 ,  C12N9/22 ,  C12N15/8241 ,  C12N15/8265

摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

摘要翻译： 一种删除植物中DNA区域的方法。 在一些实施方案中，所述方法包括用核酸分子转化植物，其中所述核酸分子编码与一个或多个组织特异性启动子可操作地连接的一个或多个锌指核酸酶（ZFN），例如， 花粉特异性启动子。 方法包括在植物中切除天然基因。 因此，在一些实施方案中，ZFN被设计为识别与天然植物基因相邻的序列。 在进一步的实施方案中，ZFN在发育阶段特异性启动子的控制下表达，使得例如在相对较晚的发育阶段在植物中特异性切除核酸序列。 包括用于实施所公开的方法的核酸分子和通过该方法生产的植物。

公开(公告)号：US09695432B2

公开(公告)日：2017-07-04

申请号：US13011666

申请日：2011-01-21

申请人： Sean M. Russell ,  Joseph F. Petolino

发明人： Sean M. Russell ,  Joseph F. Petolino

IPC分类号： C12N15/82 ,  C12N9/22 ,  A01H1/02

CPC分类号： C12N15/8213 ,  A01H1/02 ,  C12N9/22 ,  C12N15/8241 ,  C12N15/8265

摘要： A method for deleting a region of DNA in a plant. In some embodiments, the method comprises transforming a plant with a nucleic acid molecule, wherein the nucleic acid molecule encodes one or more zinc finger nuclease(s) (ZFNs) operably linked to one or more tissue-specific promoter(s), e.g., a pollen-specific promoter. Methods include excising native genes in a plant. Accordingly, in some embodiments, ZFNs are engineered that recognize sequences that flank native plant genes. In further embodiments, ZFNs are expressed under the control of developmental stage-specific promoters, such that, for example, nucleic acid sequences are specifically excised in plants during relatively late stages of development. Nucleic acid molecules useful for carrying out disclosed methods and plants produced by the methods are included.

公开(公告)号：US09617551B2

公开(公告)日：2017-04-11

申请号：US13812469

申请日：2011-07-29

申请人： Donald J. Merlo ,  Sean M. Russell ,  Diane M. Retallack ,  Aaron T. Woosley ,  Thomas Meade ,  Kenneth Narva

发明人： Donald J. Merlo ,  Sean M. Russell ,  Diane M. Retallack ,  Aaron T. Woosley ,  Thomas Meade ,  Kenneth Narva

IPC分类号： C12N15/82 ,  C12N15/74

CPC分类号： C12N15/8205 ,  C07K14/195 ,  C07K14/325 ,  C12N15/74 ,  C12N15/743 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

公开(公告)号：US08163876B2

公开(公告)日：2012-04-24

申请号：US12299404

申请日：2007-06-08

申请人： Matthew Henry ,  Ignacio M. Larrinua ,  Sean M. Russell

发明人： Matthew Henry ,  Ignacio M. Larrinua ,  Sean M. Russell

IPC分类号： A01H5/00 ,  C07K7/00 ,  A61K39/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/04

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/517 ,  A61K2039/545 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/8258 ,  C12N2760/16122 ,  C12N2760/16134

摘要： The subject invention provides novel amino acid sequences (including a consensus sequence) of the Avian Influenza A virus hemagglutinin protein. These newly constructed genes are designed to provide a broader spectrum of activity across the serotype family thus providing a basis for a vaccine that has broad heterologous disease protection. The novel genes have been further improved by the addition of strategic glycosylation sites into the amino acids sequences that they encode. These genes can also, optionally, be codon optimized for plant expression, inserted into the appropriate vector and cloned into plants for expression. Polypeptides produced by recombinant host cells or transgenic plants can also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals. Additionally, transgenic plant material may also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals.

摘要翻译： 本发明提供禽流感A病毒血凝素蛋白的新型氨基酸序列（包括共有序列）。 这些新构建的基因被设计为提供跨血清型家族的更广泛的活性范围，从而为具有广泛异源疾病保护的疫苗提供基础。 通过在其编码的氨基酸序列中加入战略糖基化位点，进一步改善了这些新基因。 这些基因也可任选地优化用于植物表达的密码子，插入合适的载体中并克隆到植物中用于表达。 通过重组宿主细胞或转基因植物产生的多肽也可以用作疫苗来源，用于制定用于控制易感个体中的流感的疫苗。 另外，转基因植物材料也可以用作疫苗的来源，用于制定用于控制易感个体中的流感的疫苗。

公开(公告)号：US10752913B2

公开(公告)日：2020-08-25

申请号：US12517906

申请日：2007-12-07

申请人： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven R. Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

发明人： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven R. Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

IPC分类号： C12N15/82

摘要： The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

公开(公告)号：US20150267213A1

公开(公告)日：2015-09-24

申请号：US13812469

申请日：2011-07-29

申请人： Donald J. Merlo ,  Sean M. Russell ,  Diane M. Retallack ,  Aaron T. Woosley ,  Thomas Meade ,  Kenneth Narva

发明人： Donald J. Merlo ,  Sean M. Russell ,  Diane M. Retallack ,  Aaron T. Woosley ,  Thomas Meade ,  Kenneth Narva

IPC分类号： C12N15/82

CPC分类号： C12N15/8205 ,  C07K14/195 ,  C07K14/325 ,  C12N15/74 ,  C12N15/743 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

摘要翻译： 提供了在能够独立于农杆菌染色体，Ti质粒和植物转化双向载体复制的质粒上携带转化增强基因的土壤杆菌菌株，以及这些农杆菌菌株的用途。 另外，缺乏DNA重组功能的农杆菌菌株导致植物转化双向载体的不稳定或重排，并且能够独立于农杆菌染色体，Ti质粒和植物转化二元体的能够复制的质粒上转录增强基因 还提供了这些菌株的载体和用途。 还包括农杆菌菌株，其在不干扰或以其他方式损害农杆菌细胞的正常生长和植物转化能力的位点处将整合到农杆菌染色体中的转化增强基因并用于这些农杆菌菌株。 还描述了使用这些农杆菌菌株制备的植物。

公开(公告)号：US07795395B2

公开(公告)日：2010-09-14

申请号：US12553662

申请日：2009-09-03

申请人： Patricia C. Apel-Birkhold ,  Timothy D. Hey ,  Joel J. Sheets ,  Thomas Meade ,  Ze-Sheng Li ,  Justin M. Lira ,  Sean M. Russell ,  Robin L. Thompson ,  Jon C. Mitchell ,  Kristin Fencil

发明人： Patricia C. Apel-Birkhold ,  Timothy D. Hey ,  Joel J. Sheets ,  Thomas Meade ,  Ze-Sheng Li ,  Justin M. Lira ,  Sean M. Russell ,  Robin L. Thompson ,  Jon C. Mitchell ,  Kristin Fencil

IPC分类号： A01H5/00 ,  C12N15/00 ,  C12N15/31 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07H21/04 ,  C07K14/24 ,  C12N15/8279 ,  Y02A40/162 ,  Y10S530/825

摘要： The invention relates to isolated genes encoding Xenorhabdus toxin complex (TC) proteins, and transgenic plants and host cells expressing Xenorhabdus toxin complex (TC) proteins and having resistance to insects.

摘要翻译： 本发明涉及编码Xenorhabdus毒素复合物（TC）蛋白质的分离基因，以及表达Xenorhabdus毒素复合物（TC）蛋白质并具有抗昆虫的转基因植物和宿主细胞。

公开(公告)号：US07585944B2

公开(公告)日：2009-09-08

申请号：US11857073

申请日：2007-09-18

申请人： Patricia C. Apel-Birkhold ,  Timothy D. Hey ,  Joel J. Sheets ,  Thomas Meade ,  Ze-Sheng Li ,  Justin M. Lira ,  Sean M. Russell ,  Robin L. Thompson ,  Jon C. Mitchell ,  Kristin Fencil

发明人： Patricia C. Apel-Birkhold ,  Timothy D. Hey ,  Joel J. Sheets ,  Thomas Meade ,  Ze-Sheng Li ,  Justin M. Lira ,  Sean M. Russell ,  Robin L. Thompson ,  Jon C. Mitchell ,  Kristin Fencil

IPC分类号： C07K1/00 ,  C07K14/00 ,  C07K14/20

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07H21/04 ,  C07K14/24 ,  C12N15/8279 ,  Y02A40/162 ,  Y10S530/825

摘要： The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus bovienii strain ILM104.

摘要翻译： 本发明涉及新型异戊酰梭菌毒素复合物（TC）蛋白质和编码这些蛋白质的基因。 更具体地，本发明涉及从Xenorhabdus bovienii菌株ILM104获得的TC基因和蛋白质。

公开(公告)号：US20110107455A1

公开(公告)日：2011-05-05

申请号：US12517906

申请日：2007-12-07

申请人： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven Robert Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

发明人： Justin M. Lira ,  Terry R. Wright ,  Sean M. Russell ,  Donald J. Merlo ,  Steven Robert Webb ,  Nicole L. Arnold ,  Andrew E. Robinson ,  Kelley A. Smith

IPC分类号： C12N15/82 ,  C12N5/10 ,  C12N15/63 ,  C12Q1/04 ,  A01H5/10 ,  A01H1/00 ,  C12N9/10 ,  C12N1/21 ,  A01N57/18 ,  C12Q1/68 ,  A01H5/00

摘要： The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

摘要翻译： 本发明涉及本文称为DSM-2的新基因。 该基因在天竺葵A3中鉴定。 DSM-2蛋白与PAT和BAR密切相关。 本发明还提供编码DSM-2蛋白质的植物优化基因，DSM-2可用作转基因性状，以将植物和植物细胞中的耐受性赋予除草剂草铵膦和双丙二酸。 主题基因的一个优选用途是选择标记。 在细菌系统中使用该基因作为选择标记可以提高植物转化的效率。 使用DSM-2作为唯一选择标记，在克隆过程中不需要额外的药用抗生素标记（如氨苄青霉素抗性）。 根据本发明，各种其他用途也是可能的。

公开(公告)号：US20090106864A1

公开(公告)日：2009-04-23

申请号：US12299404

申请日：2007-06-08

申请人： Matthew Henry ,  Ignacio M. Larrinua ,  Sean M. Russell

发明人： Matthew Henry ,  Ignacio M. Larrinua ,  Sean M. Russell

IPC分类号： A01H5/00 ,  C07K7/00 ,  A61K39/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/04

CPC分类号： A61K39/145 ,  A61K39/12 ,  A61K2039/517 ,  A61K2039/545 ,  C07K14/005 ,  C07K2319/00 ,  C12N15/8258 ,  C12N2760/16122 ,  C12N2760/16134

摘要： The subject invention provides novel amino acid sequences (including a consensus sequence) of the Avian Influenza A virus hemagglutinin protein. These newly constructed genes are designed to provide a broader spectrum of activity across the serotype family thus providing a basis for a vaccine that has broad heterologous disease protection. The novel genes have been further improved by the addition of strategic glycosylation sites into the amino acids sequences that they encode. These genes can also, optionally, be codon optimized for plant expression, inserted into the appropriate vector and cloned into plants for expression. Polypeptides produced by recombinant host cells or transgenic plants can also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals. Additionally, transgenic plant material may also be used as source of antigen for the formulation of vaccines for the control of influenza in susceptible individuals.

摘要翻译： 本发明提供禽流感A病毒血凝素蛋白的新型氨基酸序列（包括共有序列）。 这些新构建的基因被设计为提供跨血清型家族的更广泛的活性范围，从而为具有广泛异源疾病保护的疫苗提供基础。 通过在其编码的氨基酸序列中加入战略糖基化位点，进一步改善了这些新基因。 这些基因也可任选地优化用于植物表达的密码子，插入合适的载体中并克隆到植物中用于表达。 通过重组宿主细胞或转基因植物产生的多肽也可以用作疫苗来源，用于制定用于控制易感个体中的流感的疫苗。 另外，转基因植物材料也可以用作疫苗的来源，用于制定用于控制易感个体中的流感的疫苗。