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36 条记录 (耗时 0.042 秒)

    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member
    2.
    发明授权
    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member 失效
    发光工具，其辅助构件和保存在工具中使用的生物发光组合物和辅助构件的方法

    公开(公告)号：US06521304B1

    公开(公告)日：2003-02-18

    申请号：US09331292

    申请日：1999-06-18

    申请人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    发明人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    IPC分类号： F21K206

    CPC分类号： C09K11/07 ,  F21K2/06

    摘要： The present invention relates to luminescent playthings such as candles, table lights, pen lights, illumination, illuminating playthings for camp, illuminating playthings for night fishing, fish-gathering lamps, safety candles, neon lights, luminescent inks, luminescent pens, luminescent coatings or luminescent writing implements, in which a soft light of an indescribable tone of color not obtainable from flames of conventional candles is emitted via seawater, lake and marsh water, river water, ground water, tap water or mineral drinking water by utilizing 3 components of luciferin, luciferase and ATP or 4 components of these components plus a metal salt, out of components necessary for bioluminescence in fireflies.

    摘要翻译： 本发明涉及诸如蜡烛，台灯，笔灯，照明，营地的照明玩具，夜间钓鱼的照明玩具，聚光灯，安全蜡烛，霓虹灯，发光油墨，发光笔，发光涂层或发光的发光玩具 发光书写工具，其中可以通过海水，湖泊和沼泽水，河水，地下水，自来水或矿物饮用水通过利用荧光素3的三种成分从普通蜡烛的火焰中获得难以形容的色调的柔和光线 ，荧光素酶和ATP或这些组分的4种组分加上金属盐，不仅仅是萤火虫生物发光所必需的组分。

    Purified luciferase from luciola lateralis
    3.
    发明授权
    Purified luciferase from luciola lateralis 失效
    来自luciola lateralis的纯化萤光素酶

    公开(公告)号：US5352598A

    公开(公告)日：1994-10-04

    申请号：US759814

    申请日：1991-08-29

    申请人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    发明人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N1/00

    CPC分类号： C12N9/0069 ,  C12Y113/12007 ,  Y10S435/815 ,  Y10S435/816

    摘要： A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.

    摘要翻译： 公开了一种来自Luciola lateralis的纯化的萤光素酶。 该酶的特征在于具有以下特性：最佳pH范围为7.5至9.5，最适温度范围为0℃至50℃，并且该酶不作用于ADP，CTP，UTP和GTP。 通过使用包括凝胶过滤色谱法，羟基磷灰石柱色谱法和三（羟基）氨基甲烷 - 氢氯酸缓冲液的方法纯化酶。

    Variant E1 protein gene for pyruvate dehydrogenase complex and variant E1 protein of pyruvate dehydrogenase complex
    7.
    发明授权
    Variant E1 protein gene for pyruvate dehydrogenase complex and variant E1 protein of pyruvate dehydrogenase complex 失效
    丙酮酸脱氢酶复合物的变体E1蛋白基因和丙酮酸脱氢酶复合体的变体E1蛋白

    公开(公告)号：US5432071A

    公开(公告)日：1995-07-11

    申请号：US215709

    申请日：1994-03-22

    申请人： Toshio Ichikawa ,  Yasuji Koyama ,  Hideko Otake ,  Eiichi Nakano

    发明人： Toshio Ichikawa ,  Yasuji Koyama ,  Hideko Otake ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N15/09 ,  C12R1/19 ,  C12N9/04 ,  C12N15/53 ,  C12N15/70

    CPC分类号： C12N9/0008 ,  C12Y102/04001 ,  C12Y102/05001

    摘要： The present invention relates to variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity in which arginine at the 146-position is replaced by proline in the amino acid sequence of wild-type E.sub.1 protein of pyruvate dehydrogenase complex, a gene coding for said variant E.sub.1 protein, a novel recombinant DNA comprising said variant E.sub.1 protein-encoding gene inserted in a vector DNA, and a process for producing variant E.sub.1 protein by said recombinant DNA. The present invention provides the variant E.sub.1 protein of pyruvate dehydrogenase complex of high activity.

    摘要翻译： 本发明涉及高活性的丙酮酸脱氢酶复合物的变体E1蛋白，其中146位的精氨酸被丙酮酸脱氢酶复合物的野生型E1蛋白的氨基酸序列中的脯氨酸替代，编码所述变体E1的基因 蛋白质，包含插入载体DNA中的所述变体E1蛋白质编码基因的新型重组DNA，以及由所述重组DNA产生变体E1蛋白质的方法。 本发明提供了具有高活性的丙酮酸脱氢酶复合物的变体E1蛋白。

    Method of controlling electrocoating bath and apparatus therefor
    8.
    发明授权
    Method of controlling electrocoating bath and apparatus therefor 失效
    电泳浴的控制方法及其设备

    公开(公告)号：US4501649A

    公开(公告)日：1985-02-26

    申请号：US548139

    申请日：1983-11-02

    申请人： Nobuo Furuno ,  Yoshio Ota ,  Masahiro Itai ,  Akio Tokuyama ,  Eiichi Nakano

    发明人： Nobuo Furuno ,  Yoshio Ota ,  Masahiro Itai ,  Akio Tokuyama ,  Eiichi Nakano

    IPC分类号： C25D13/24

    CPC分类号： C25D13/24

    摘要： The present invention provides a method of controlling an electrocoating bath using an ion exchange resin. The ion exchange resin is maintained in a porous container in a suspended state in the electrocoating bath, and is used in a quantity of not more than the chemical equivalent of excess counter-ion to be removed from the bath. Bath control is easily achieved without any coagulation trouble as often observed in conventional column methods.

    摘要翻译： 本发明提供一种使用离子交换树脂控制电泳浴的方法。 离子交换树脂在电镀浴中以悬浮状态保持在多孔容器中，并且使用的量不超过要从浴中除去的过量抗衡离子的化学当量。 在常规柱方法中经常观察到的浴控制容易实现而没有任何凝结问题。

    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter
    9.
    发明授权
    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter 失效
    含有鞣酸酶基因的DNA片段，重组质粒，鞣酸酶的制备方法和启动子

    公开(公告)号：US5665584A

    公开(公告)日：1997-09-09

    申请号：US460860

    申请日：1995-06-05

    申请人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    发明人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N9/18 ,  C12N15/00 ,  C12R1/69

    CPC分类号： C12N9/18

    摘要： The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.

    摘要翻译： 本发明涉及含有编码鞣酸酶的基因并衍生自属于曲霉属的微生物的3,563个碱基对的DNA片段，其具有以下限制酶图：B：Bam HI，H：Hind III，K ：Kpn I，S：Sal I，X：Xba I; 含有编码（SEQ ID NO：4）的氨基酸序列的鞣酸酶基因的DNA片段; 包含插入质粒载体中的含有鞣酸酶基因的DNA片段的重组质粒; 一种鞣酸酶的制造方法，其特征在于，在培养基中培养能够在培养基中生产鞣酸酶的微生物属于属于曲霉属的微生物，从培养物中回收鞣酸酶; 和由（SEQ ID NO：1）的核苷酸序列表示的启动子。 根据本发明可以有效地制备鞣酸酶。

    Method for continuous pretreatment of a cloth
    10.
    发明授权
    Method for continuous pretreatment of a cloth 失效
    连续预处理布的方法

    公开(公告)号：US5334224A

    公开(公告)日：1994-08-02

    申请号：US25841

    申请日：1993-03-03

    申请人： Yoshiteru Sando ,  Eiichi Nakano ,  Hiroshi Ishidoshiro ,  Koji Sando

    发明人： Yoshiteru Sando ,  Eiichi Nakano ,  Hiroshi Ishidoshiro ,  Koji Sando

    IPC分类号： D06B3/12 ,  D06L1/14 ,  D06L3/06 ,  D06L3/08 ,  D06L3/14

    CPC分类号： D06L3/08 ,  D06L1/14

    摘要： A method for the pretreatment of a long cloth continuously in which a cloth to be subjected to desizing, scouring and bleaching for the pretreatment thereof is preliminarily subjected to souring treatment in the sodium chlorite solution prepared by absorbing the chlorine dioxide gas, which is exhausted from the subsequent bleaching process with the use of chlorite, in a solution of H.sub.2 O.sub.2 and NaOH, and accordingly it is possible to transport the long cloth continuously in the pretreating process speedily with no need of staying the cloth in folded state for increasing the productivity and improving the quality of the product, and further to render the pretreating apparatus compact so as suitable for the production of small lot products.

    摘要翻译： 一种连续预处理长纤维的方法，其中待进行退浆，精练和漂白处理的布预先在吸收二氧化氯气体制备的亚氯酸钠溶液中进行酸处理，该二氧化氯溶液是从 随后的使用亚氯酸盐的漂白工艺在H2O2和NaOH的溶液中，因此可以快速地在预处理过程中连续输送长布，而不需要将布留在折叠状态，以提高生产率和改善 产品的质量，进一步使预处理装置紧凑，从而适合生产小批量产品。