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公开(公告)号：US20100043882A1
公开(公告)日：2010-02-25
申请号：US12308434
申请日：2007-06-15
申请人： Setsuya Sato , Toshiyuki Yamato , Katsuaki Hayashi , Yoshitaka Matsumoto , Takahiro Ochiya , Isamu Oh
发明人： Setsuya Sato , Toshiyuki Yamato , Katsuaki Hayashi , Yoshitaka Matsumoto , Takahiro Ochiya , Isamu Oh
IPC分类号： F16L41/00
CPC分类号： C12M33/00 , B01L3/502761 , G01N15/1056 , Y10T137/0318 , Y10T137/85938
摘要： [Problems]To provide a microchip for cell arranging which transfers and arranges the cell accurately and quickly, by combining advantages of the method of an optical tweezer and of micro channel.[Means for Solving Problems]A microchip for arranging cells, including a base, the base comprising: at least one cell supply channel including a cell inlet port in liquid communication with a supply mechanism configured to supply the cells into the cell supply channel; at least one cell drain channel including a cell drain port in fluid communication with a drain mechanism, the cell drain channel configured to guide the cells to the cell drain port; at least one reservoir for temporarily storing the cells flowing between the cell supply channel and the cell drain channel; at least one cell arrangement portion for receiving cells introduced from the reservoir, the cell arrangement portion comprising plural compartments partitioned by a wall; and at least one junction channel extending from the cell arrangement portion to the reservoir; wherein the reservoir is wider than the cell supply channel, the cell drain channel and the junction channel.
摘要翻译： [问题]通过组合光镊方法和微通道的优点，提供一种用于细胞排列的微芯片，其通过精确而快速地传送和排列细胞。 解决问题的手段一种用于布置电池的微芯片，包括基座，所述基座包括：至少一个电池供应通道，包括与供配给将电池供应到电池供应通道的供电机构液体连通的电池入口; 至少一个单元排放通道，包括与漏极机构流体连通的单元排出端口，所述单元排放通道被配置为将单元引导到单元排放端口; 至少一个存储器，用于临时存储在单元供应通道和单元排放通道之间流动的单元; 至少一个用于接收从储存器引入的单元的单元布置部分，单元布置部分包括由壁分隔的多个隔室; 以及从单元布置部分延伸到储存器的至少一个接合通道; 其中所述储存器比所述电池供给通道，所述电池排出通道和所述接合通道宽。
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公开(公告)号：US20050276456A1
公开(公告)日：2005-12-15
申请号：US11139015
申请日：2005-05-26
CPC分类号： G06K9/00127 , C12M41/36 , C12M47/04 , G02B21/365
摘要： The present invention provides a cell-operating device comprising a laser light source and a microscope to observe cell sample fluorescently labeled and a camera to take the images of the cell sample that is observed with said microscope and a computer to capture the digital image data obtained by said camera and a group of mirrors comprising electronic control mirrors of which optical axis can be adjusted so that the laser light irradiated from said laser light source can be guided in to said microscope. The computer includes a binarization program for binarizing said digital image data by identifying whether each pixel of said digital image data captures a part of said cell sample according to the fluorescence luminance of each pixel. The computer has an identification program for identifying the boundaries between adjacent different values in the binarized digital image and joins the boundaries into an isolated closed curve, and a data processing program for processing said binarized digital data so that in all the area within said isolated closed curve a cell exists, and a calculation program to divide said binarized digital image data, after running said data processing program, into sections with a predefined size and identify the center of gravity coordinate of each section, and an adjustment program to adjust the optical axis of said electronic control mirrors so that said laser irradiates said center of gravity coordinate. Thus, the present invention is a cell-operating device that the laser light from said laser light source irradiates said center of gravity coordinate.
摘要翻译： 本发明提供了一种细胞操作装置，其包括激光光源和显微镜以观察荧光标记的细胞样品，以及相机拍摄用所述显微镜观察的细胞样品的图像和计算机，以捕获获得的数字图像数据 通过所述相机和一组镜子，包括可以调节光轴的电子控制反射镜，使得可以将从所述激光源照射的激光引导到所述显微镜。 计算机包括二进制化程序，用于通过识别所述数字图像数据的每个像素是否根据每个像素的荧光亮度捕获所述细胞样本的一部分来二值化所述数字图像数据。 计算机具有用于识别二进制数字图像中的相邻不同值之间的边界的识别程序，并将边界连接成隔离闭合曲线，以及用于处理所述二进制数字数据的数据处理程序，使得在所述隔离闭合的所有区域内 曲线单元存在，以及计算程序，用于在运行所述数据处理程序之后将所述二进制数字图像数据划分成具有预定尺寸的区段，并且识别每个区段的重心坐标，以及调整程序以调整光轴 的所述电子控制反射镜，使得所述激光器照射所述重心坐标。 因此，本发明是一种电池操作装置，来自所述激光光源的激光照射所述重心坐标。
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公开(公告)号：US20100066880A1
公开(公告)日：2010-03-18
申请号：US12308442
申请日：2007-06-15
CPC分类号： G01N15/1484 , B01L3/502761 , B01L2200/0647 , B01L2200/0652 , B01L2300/0864 , B01L2400/0454 , B01L2400/0487 , C12M47/04 , G01N15/1459 , G01N15/1463 , G01N2015/149
摘要： [Problems] To provide a microchip for cell sorting to obtain the desired cell from a number of cells quickly and accurately by combining the advantages from both methods using the optical tweezer and the liquid supply to the micro channel.[Means for solving problems] A microchip for sorting at least one cell including a base, the base comprising: a cell supply channel for supplying and flowing a liquid containing cells; a cell supply channel including an inlet port in liquid communication with a supply mechanism and a drain port in liquid communication with a drain mechanism; a cell sorting channel intersecting with the cell supply channel at their middle point, the cell sorting channel including an inlet port in liquid communication with a supply mechanism and a drain port in liquid communication with a drain mechanism.
摘要翻译： [问题]提供一种用于细胞分选的微芯片，通过结合使用光学镊子的两种方法和向微通道的液体供应的优点，快速而准确地从许多细胞获得所需的细胞。 解决问题的手段一种用于对至少一个包括基底的电池进行分选的微型芯片，所述基底包括：用于供给和流动含有细胞的液体的电池供给通道; 电池供应通道，其包括与供给机构液体连通的入口和与排出机构液体连通的排出口; 细胞分选通道在其中点处与细胞供应通道相交，细胞分选通道包括与供给机构液体连通的入口和与排出机构液体连通的排出口。
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公开(公告)号：US20100068794A1
公开(公告)日：2010-03-18
申请号：US12225977
申请日：2007-03-27
申请人： Isamu Oh , Hiroshi Masuhara , Toshiyuki t Yamato , Katsuaki Hayashi , Yasuji Adachi , Sato Setsuya
发明人： Isamu Oh , Hiroshi Masuhara , Toshiyuki t Yamato , Katsuaki Hayashi , Yasuji Adachi , Sato Setsuya
IPC分类号： C12M3/00
CPC分类号： C12M35/02
摘要： (Problems) To provide a cell fusion chip, which allows to perform a cell fusion on a single chip, excels in the operating efficiency, does not require experience so much in cell feed or recovery, and has no risk of the contamination to the cells during the operation.(Means for Solving Problems) The present invention provides a cell fusion chip comprising: an isolation chamber for receiving isolated cells to be subjected to an fusion operation; a fusion chamber for fusing the cells together supplied from the isolation chamber; a culture chamber for culturing the cells fused in the fusion chamber; a first channel for connecting the isolation chamber and the fusion chamber; and a second channel for connecting the fusion chamber and the culture chamber, wherein the isolation chamber, the fusion chamber, the culture chamber, the first channel and the second channel are formed on a single chip.
摘要翻译： （问题）为了提供能够在单个芯片上进行细胞融合的细胞融合芯片，其操作效率优异，在细胞进料或回收中不需要经验，并且没有细胞污染的风险 在操作期间。 （解决问题的手段）本发明提供一种细胞融合芯片，包括：隔离室，用于接收待进行融合操作的分离细胞; 用于将从隔离室供应的单元熔合的熔化室; 用于培养在所述融合室中熔融的细胞的培养室; 用于连接隔离室和熔化室的第一通道; 以及用于连接熔融室和培养室的第二通道，其中隔离室，熔化室，培养室，第一通道和第二通道形成在单个芯片上。
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公开(公告)号：USD485368S1
公开(公告)日：2004-01-13
申请号：US29176882
申请日：2003-03-03
申请人： Katsuaki Hayashi
设计人： Katsuaki Hayashi
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公开(公告)号：US20120161483A1
公开(公告)日：2012-06-28
申请号：US13046143
申请日：2011-03-11
申请人： Katsuaki Hayashi
发明人： Katsuaki Hayashi
CPC分类号： A47C1/03238 , A47C1/03255 , A47C1/03266 , A47C1/03272 , A47C1/03294 , A47C3/30
摘要： An object of this invention is to provide a chair comprising a simple and compact rocking mechanism and lock mechanism to lock the rocking movement.The chair comprises a support frame 4 supported by a support rod 12, a back frame 5 that can make an inclining movement, a seat frame 6 that make a movement in conjunction with the back frame 5 toward a direction along the inclining movement of the back frame 5, a reactive force mechanism 7 that accumulates a spring force against an interlocking movement, and a lock mechanism 8 that locks the interlocking movement, wherein the reactive force mechanism 7 comprises a spring case 71 and a spring 72 that can make a relative movement in the back and forth direction, and has such an arrangement that the spring case 71 moves in accordance with the interlocking movement while inclining together with the seat frame 6 with a movement of a part of a front end part and a rear end part of the spring case 71 limited by the seat frame 6 during the interlocking movement, and the lock mechanism 8 locks the interlocking movement of the seat frame 6 and the back frame 5 with the support frame 4 by fixing a relative position of the spring case 71 and the seat frame 6.
摘要翻译： 本发明的目的是提供一种椅子，其包括简单且紧凑的摇摆机构和锁定机构以锁定摇摆运动。 椅子包括由支撑杆12支撑的支撑框架4，可以进行倾斜运动的后框架5，使座椅框架6与后框架5一起沿朝向后部的倾斜运动的方向运动 框架5，积聚反作用力的弹簧力的反作用力机构7和锁定机构8，锁定机构8锁定互锁运动，其中反作用力机构7包括弹簧壳体71和弹簧72，弹簧壳体可以进行相对运动 并且具有这样的布置，使得弹簧壳体71随着互锁运动而移动，同时与座椅框架6一起倾斜，同时使得前端部分和后端部分的一部分的运动 在联锁运动期间由座椅框架6限制的弹簧壳体71，并且锁定机构8通过固定来锁定座椅框架6和后部框架5与支撑框架4的互锁运动 弹簧壳体71和座椅框架6的相对位置。
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公开(公告)号：US20120161482A1
公开(公告)日：2012-06-28
申请号：US13046078
申请日：2011-03-11
申请人： Katsuaki Hayashi
发明人： Katsuaki Hayashi
IPC分类号： A47C3/00
CPC分类号： A47C1/03255 , A47C1/03238 , A47C1/03266 , A47C1/03272 , A47C1/03294 , A47C3/30
摘要： An object of this invention is to provide a chair having less sliding resistance, less abrasion and less noise with prevention of partially contacting each component while the chair is making a rocking movement.The chair comprises a support frame 4 supported by a support rod 12, a back frame 5 that can make an inclining movement relative to the support frame 4, a seat frame 6 that is related to the support frame 4 and the back frame 5 so as to make a movement in conjunction with the back frame 5 toward a direction along the inclining movement of the back frame 5, and a spring case 71 arranged along the seat frame 6 in a state that a proximal point part of the spring case 71 is supported by the support frame 4 so as to introduce a spring force against a force of an interlocking movement of the seat frame 6 and the back frame 5 to the support frame 4, wherein a spring 72 is so arranged to be compressed by a movement of the seat frame 6 relative to the spring case 71, and a slide guide part 66 to make the spring case 71 and the seat frame 6 line-contact in a direction orthogonal to the back and forth direction is arranged between the spring case 71 and the seat frame 6.
摘要翻译： 本发明的目的是提供一种椅子，其具有较小的滑动阻力，较少的磨损和较小的噪音，同时防止在椅子进行摇摆运动时部分地接触每个部件。 椅子包括由支撑杆12支撑的支撑框架4，能够相对于支撑框架4进行倾斜运动的后框架5，与支撑框架4和后框架5相关的座椅框架6，从而 与背框5一起沿着后框架5的倾斜运动的方向进行移动，弹簧壳体71以弹簧壳体71的基端部被支撑的状态沿着座椅框架6配置 通过支撑框架4，以便将座椅框架6和后框架5的互锁运动的力引入弹簧力到支撑框架4，其中弹簧72被布置成被 座椅框架6相对于弹簧壳体71，以及滑动引导部66，其使得弹簧壳体71和座椅框架6在与前后方向正交的方向上线接触地布置在弹簧壳体71和座椅 框架6。
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公开(公告)号：USD294142S
公开(公告)日：1988-02-09
申请号：US707850
申请日：1985-03-04
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