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公开(公告)号:US4940836A
公开(公告)日:1990-07-10
申请号:US782385
申请日:1985-10-01
IPC分类号: C12N15/02 , A01H1/00 , A01H1/02 , A01H1/06 , A01H4/00 , A01H5/00 , C12N5/00 , C12N5/10 , C12N5/14
CPC分类号: C12N5/14 , A01H1/02 , A01H1/06 , C12N5/0025
摘要: Somatic hybrids of Rutaceae plants were obtained by allowing the protoplasts from embryogenic cells of a Rutaceae plant, which are of the nucellar origin, resistant to fusion treatment, and have a high proliferation activity, to fuse with the protoplasts of another Rutaceae plant having no proliferation activity, and then cultivating the fused protoplasts in a medium not containing a phytohormone but containing a saccharide in a concentration suitable for the selective embryogenesis of the hybrid cells.
摘要翻译: 芸香科植物的体细胞杂种通过使来自芸苔属植物的芸苔科植物的胚发生细胞的原生质体具有抗融合处理并且具有高增殖活性,与不具有增殖的另一种芸香科植物的原生质体融合 然后在不含植物激素但含有适合于混合细胞选择性胚发生的浓度的糖的培养基中培养融合的原生质体。