公开(公告)号：US4051314A

公开(公告)日：1977-09-27

申请号：US513958

申请日：1974-10-11

申请人： Shigeto Ohtsuka ,  Saburo Ueno ,  Chikao Yoshikumi ,  Fumio Hirose ,  Yoshio Ohmura ,  Toshihiko Wada ,  Takayoshi Fujii ,  Eiichi Takahashi

发明人： Shigeto Ohtsuka ,  Saburo Ueno ,  Chikao Yoshikumi ,  Fumio Hirose ,  Yoshio Ohmura ,  Toshihiko Wada ,  Takayoshi Fujii ,  Eiichi Takahashi

IPC分类号： A61K31/715 ,  A61K36/07 ,  A61K36/899 ,  C08B37/00 ,  C12P19/04

CPC分类号： C12P19/04 ,  A61K31/715 ,  A61K36/07 ,  A61K36/899 ,  C08B37/006 ,  Y10S435/803 ,  Y10S435/911

摘要： Polysaccharides are produced by purifying a liquid extract of a mycelium of a strain of fungus species belonging to the class Basidiomycetes or the filtered broth of a cultured medium in which a mycelium of a selected strain of Basidiomycetes has been incubated; the polysaccharides exhibiting an anticarcinogenic activity in mice.

公开(公告)号：US4851395A

公开(公告)日：1989-07-25

申请号：US163238

申请日：1988-02-26

申请人： Saburo Ueno ,  Chikao Yoshikumi ,  Yoshio Omura ,  Takayoshi Fujii ,  Toshihiko Wada ,  Eiichi Takahashi ,  Fumio Hirose

发明人： Saburo Ueno ,  Chikao Yoshikumi ,  Yoshio Omura ,  Takayoshi Fujii ,  Toshihiko Wada ,  Eiichi Takahashi ,  Fumio Hirose

IPC分类号： A61K36/07 ,  C12P21/00

CPC分类号： C12P21/005 ,  A61K36/07

摘要： Disclosed is nitrogen-containing polysaccharide substantially free of units having a molecular weight below about 5,000, said nitrogen-containing polysaccharide having been extracted from Coriolus versicolor (Fr.) Quel., substantially all polymer units having a molecular weight below about 5,000 having been removed therefrom, which exhibits antitumor and immunological activity.

摘要翻译： 公开了基本上不含分子量低于约5,000的单元的含氮多糖，所述含氮多糖已从Coriolus versicolor（Fr.）Quel提取，基本上分子量低于约5,000的聚合物单元已经被除去 其具有抗肿瘤和免疫活性。

公开(公告)号：US4699787A

公开(公告)日：1987-10-13

申请号：US585037

申请日：1984-03-06

申请人： Saburo Ueno ,  Chikao Yoshikumi ,  Yoshio Omura ,  Takayoshi Fujii ,  Toshihiko Wada ,  Eiichi Takahashi ,  Fumio Hirose

发明人： Saburo Ueno ,  Chikao Yoshikumi ,  Yoshio Omura ,  Takayoshi Fujii ,  Toshihiko Wada ,  Eiichi Takahashi ,  Fumio Hirose

IPC分类号： C12P19/04 ,  A61K31/715 ,  A61K35/66 ,  A61K36/06 ,  A61K36/07 ,  A61K36/074 ,  A61P35/00 ,  C08B37/00 ,  C12P1/02 ,  C12P19/00 ,  C12P21/00 ,  C12R1/645 ,  C07H1/08

CPC分类号： A61K36/074 ,  C12P21/005 ,  Y10S514/893

摘要： A method of restoring the immune response of a patient having a lowered immune response level is provided, in which the patient is given an effective amount of certain nitrogen-containing polysaccharides derived from Coriolus versicolor (Fr.) Quel, where the polymer units having a molecular weight of below about 5,000 have been removed therefrom.

摘要翻译： 提供一种恢复具有降低的免疫应答水平的患者的免疫应答的方法，其中给予患者有效量的衍生自Coriolus versicolor（Fr.）Quel的某些含氮多糖，其中聚合物单元具有 小于约5,000的分子量已被除去。

公开(公告)号：US4229570A

公开(公告)日：1980-10-21

申请号：US788991

申请日：1977-04-19

申请人： Saburo Ueno ,  Chikao Yoshikumi ,  Fumio Hirose ,  Yoshio Omura ,  Toshihiko Wada ,  Takayoshi Fujii ,  Eiichi Takahashi

发明人： Saburo Ueno ,  Chikao Yoshikumi ,  Fumio Hirose ,  Yoshio Omura ,  Toshihiko Wada ,  Takayoshi Fujii ,  Eiichi Takahashi

IPC分类号： C12P19/04 ,  A61K31/715 ,  A61K35/66 ,  A61K36/06 ,  A61K36/07 ,  A61K36/074 ,  A61P35/00 ,  C08B37/00 ,  C12P1/02 ,  C12P19/00 ,  C12P21/00 ,  C12R1/645 ,  A61K31/73 ,  C07H1/08

CPC分类号： A61K36/074 ,  C12P21/005

摘要： The fungi belonging to the Coriolus genus is first extracted with water or a dilute alkaline solution and then further extracted stepwise by using alkaline solutions with gradually increased concentrations to obtain a nitrogen-containing polysaccharide having an anti-tumor activity in a high yield.

摘要翻译： 首先用水或稀碱溶液提取属于科氏菌属的真菌，然后通过逐渐浓缩的碱性溶液逐步提取，以高产率获得具有抗肿瘤活性的含氮多糖。

公开(公告)号：US4202969A

公开(公告)日：1980-05-13

申请号：US788992

申请日：1977-04-19

申请人： Saburo Ueno ,  Chikao Yoshikumi ,  Fumio Hirose ,  Yoshio Omura ,  Toshihiko Wada ,  Takayoshi Fujii ,  Eiichi Takahashi

发明人： Saburo Ueno ,  Chikao Yoshikumi ,  Fumio Hirose ,  Yoshio Omura ,  Toshihiko Wada ,  Takayoshi Fujii ,  Eiichi Takahashi

IPC分类号： C12P19/04 ,  A61K31/715 ,  A61K35/66 ,  A61K36/06 ,  A61K36/07 ,  A61K36/074 ,  A61P35/00 ,  C08B37/00 ,  C12P1/02 ,  C12P19/00 ,  C12P21/00 ,  C12R1/645 ,  C07H1/08

CPC分类号： A61K36/074 ,  C12P21/005 ,  Y10S514/893

摘要： The fungi belonging to the Coriolus genus are extracted by using a dilute alkaline solution having a concentration within a certain specified range whereby the low molecular weight substances with molecular weight of less than 5,000 are obtained in there extract solution. These low molecular weight substances are separated from the extract solution to obtain a nitrogen-containing polysaccharide having excellent anti-tumor activity.

摘要翻译： 通过使用浓度在一定范围内的稀碱性溶液提取属于科氏菌属的真菌，由此在提取液中获得分子量小于5,000的低分子量物质。 从提取液中分离出这些低分子量物质，得到抗肿瘤活性优异的含氮多糖。

公开(公告)号：US4472907A

公开(公告)日：1984-09-25

申请号：US423103

申请日：1982-09-24

申请人： Toshihiko Wada ,  Akiyoshi Nakashima ,  Azuma Okubo ,  Yoshio Ohmura ,  Chikao Yoshikumi

发明人： Toshihiko Wada ,  Akiyoshi Nakashima ,  Azuma Okubo ,  Yoshio Ohmura ,  Chikao Yoshikumi

IPC分类号： A01G1/04

CPC分类号： A01G1/04

摘要： A fruit body of Ganoderma lucidum (Fr.) Karst. having a long stipe is prepared by the method of the invention comprising the following steps;(a) cultivating a seed culture of a Ganoderma lucidum (Fr.) Karst. strain in an artificial solid medium to obtain a fungal tissue bed,(b) maintaining the fungal tissue bed at a humidity of at least 90% and an illumination intensity of at most 500 l.times. to form selectively a primordium of fruit body from the fungal tissue bed, and(c) maintaining the resulting culture at a humidity of from 40 to lower than 90% and an illumination intensity of at least 500 l.times. to form a fungal pileus of the fruit body from the primordium.

摘要翻译： 灵芝（Fr.）卡斯特的果实体。 通过包括以下步骤的本发明的方法制备具有长柄的： （a）培育灵芝（Fr.）Karst的种子培养物。 在人造固体培养基中应变以获得真菌组织床，（b）将真菌组织床保持在至少90％的湿度和至多500lx的照射强度，以从真菌组织中选择性地形成果实原基 床和（c）将所得培养物保持在40至低于90％的湿度和至少500lx的照射强度以从原基形成果实的真菌堆积物。

公开(公告)号：US4288555A

公开(公告)日：1981-09-08

申请号：US104400

申请日：1979-12-17

申请人： Chikao Yoshikumi ,  Toshihiko Wada ,  Himromitsu Makita ,  Kinzaburo Suzuki

发明人： Chikao Yoshikumi ,  Toshihiko Wada ,  Himromitsu Makita ,  Kinzaburo Suzuki

IPC分类号： A01G1/04 ,  C12N1/00 ,  C12N1/14 ,  C12R1/645

CPC分类号： C12N1/14 ,  Y10S435/803

摘要： In submerged culture of a Basidiomycete belonging to the genus Coriolus, the seed culture to be inoculated into the medium is homogenized to form a homogenous slurry and the slurry-formed seed culture is inoculated into the medium. This improves the cultivation rate of the Basidiomycetes.

摘要翻译： 在属于科里奥氏菌属的担子菌的浸没培养物中，将接种到培养基中的种子培养物均质化以形成均匀的浆液，并将浆液形成的种子培养物接种到培养基中。 这提高了担子菌的培养率。

公开(公告)号：US4159225A

公开(公告)日：1979-06-26

申请号：US826118

申请日：1977-08-19

申请人： Chikao Yoshikumi ,  Yoshio Omura ,  Toshihiko Wada ,  Hiromitsu Makita ,  Takao Ando ,  Noriyuki Toyoda ,  Kenichi Matsunaga

发明人： Chikao Yoshikumi ,  Yoshio Omura ,  Toshihiko Wada ,  Hiromitsu Makita ,  Takao Ando ,  Noriyuki Toyoda ,  Kenichi Matsunaga

IPC分类号： C12N1/14 ,  A01H15/00 ,  A23K1/16 ,  A23L1/28 ,  A61K36/07 ,  A61P35/00 ,  A61P35/02 ,  C05F11/08 ,  C12N1/00 ,  C12N9/30 ,  C12N9/58 ,  C12N15/02 ,  C12P19/04 ,  C12P21/00 ,  C12D13/04 ,  C12B1/08 ,  C12K1/00

CPC分类号： C12P21/005 ,  C12N15/02 ,  Y10S435/911

摘要： When a dikaryotic mycelium of Coriolus versicolor (Fr.) Quel. (a species of fungi belonging to the genus Coriolus of Polyporaceae) is subjected to a mechanical treatment such as grinding or shearing in a liquid medium, or when such mycelium is subjected to submerged culture while undergoing mechanical treatment, there is produced a monokaryotic mycelium which is different from the dikaryotic mycelium in morphological and physiological characteristics. The thus obtained monokaryotic mycelium is a novel product and characterized by its extremely high propagation rate as compared with the known dikaryotic mycelium. Also, the substance extracted from the culture of the mycelium has excellent physiological activities.

摘要翻译： 当科里奥利（Coriolus versicolor）（Fr.）Quel的原核菌丝体。 （属于多孔科科氏菌属的一类真菌）在液体培养基中进行机械处理如研磨或剪切，或者当经过机械处理使这种菌丝体进行浸没培养时，产生单核菌丝体， 在形态和生理特征上与原核菌丝体不同。 由此获得的单核菌丝体是一种新颖的产物，其特征在于与已知的二核体系相比具有极高的增殖速率。 此外，从菌丝体培养物中提取的物质具有优异的生理活性。

公开(公告)号：US4140578A

公开(公告)日：1979-02-20

申请号：US817226

申请日：1977-07-20

申请人： Chikao Yoshikumi ,  Toshihiko Wada ,  Masahiko Fujii ,  Hiromitsu Makita ,  Kinzaburo Suzuki ,  Akio Shinmyo ,  Haruhisa Hayashi

发明人： Chikao Yoshikumi ,  Toshihiko Wada ,  Masahiko Fujii ,  Hiromitsu Makita ,  Kinzaburo Suzuki ,  Akio Shinmyo ,  Haruhisa Hayashi

IPC分类号： C12P19/04 ,  A61K36/06 ,  A61K36/07 ,  A61P35/00 ,  C07G11/00 ,  C08B37/00 ,  C12P19/00 ,  C12P21/00 ,  C12R1/645 ,  C12D13/04

CPC分类号： C12P21/005 ,  A61K36/07 ,  Y10S435/911

摘要： A fungus belonging to genus Coriolus of the class Basidiomycetes is subjected to submerged culture in a liquid medium and the obtained culture is dried and extracted with an aqueous solvent, and then the substances with molecular weights of less than 5,000 are eliminated from the extract solution, whereby a polysaccharide having anti-cancer and other pharmacodynamic effects is obtained in a high yield.

摘要翻译： 将属于担子菌纲的科里氏菌属的真菌在液体培养基中进行浸没培养，并将所得培养物干燥并用水性溶剂萃取，然后从提取液中除去分子量小于5,000的物质， 从而获得高产率的具有抗癌和其它药效学作用的多糖。

公开(公告)号：US4237233A

公开(公告)日：1980-12-02

申请号：US819401

申请日：1977-07-26

申请人： Chikao Yoshikumi ,  Toshihiko Wada ,  Hiromitsu Makita ,  Kinzaburo Suzuki ,  Azuma Okubo ,  Takishi Nakanoya ,  Katsunori Miura ,  Tadao Sagi

发明人： Chikao Yoshikumi ,  Toshihiko Wada ,  Hiromitsu Makita ,  Kinzaburo Suzuki ,  Azuma Okubo ,  Takishi Nakanoya ,  Katsunori Miura ,  Tadao Sagi

IPC分类号： C12N1/14 ,  C12P19/04

CPC分类号： C12N1/14 ,  C12P19/04 ,  Y10S435/812 ,  Y10S435/911

摘要： In cultivation of the Basidiomycetes under aeration and agitation in an aqueous liquid medium containing saccharide as a carbon source and yeast extract, peptone, casamino acid and/or meat extract as nutrient, the initial charge of the medium is adjusted such that it is less than about 70% of the capacity of the fermentor used, and the medium or at least one component thereof is supplied additionally in portions as cultivation advances. Such control of the medium feed suppresses excessive foaming which occurs in the aqueous medium during cultivation, and allows smooth and efficient cultivation of the Basidiomycetes.

摘要翻译： 在含有糖源和酵母提取物，蛋白胨，酪蛋白胨和/或肉提取物作为营养物质的含水液体培养基中，在通气和搅拌下培养担子菌，培养基的初始电荷调节至小于 约70％的发酵罐使用能力，并且中等或其至少一种成分在培育进展时以额外的方式供应。 中等进料的这种控制抑制了在培养期间在水性介质中发生的过度起泡，并且能够顺利且有效地培育担子菌。