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    METHOD FOR INTRODUCING MUTANT GENE, GENE HAVING MUTATION INTRODUCED THEREIN, CASSETTE FOR INTRODUCING MUTATION, VECTOR FOR INTRODUCING MUTATION, AND KNOCK-IN NON-HUMAN MAMMALIAN ANIMAL
    10.
    发明申请
    METHOD FOR INTRODUCING MUTANT GENE, GENE HAVING MUTATION INTRODUCED THEREIN, CASSETTE FOR INTRODUCING MUTATION, VECTOR FOR INTRODUCING MUTATION, AND KNOCK-IN NON-HUMAN MAMMALIAN ANIMAL 审中-公开
    引入突变基因的方法，引入突变体的基因，引入突变的CASSETTE，引入突变的载体和非人类哺乳动物动物

    公开(公告)号：US20120042401A1

    公开(公告)日：2012-02-16

    申请号：US13125775

    申请日：2009-10-23

    申请人： Shinichi Hirose ,  Masanobu Deshimaru ,  Kimi Araki

    发明人： Shinichi Hirose ,  Masanobu Deshimaru ,  Kimi Araki

    IPC分类号： C12N15/85 ,  C12P19/34

    CPC分类号： A01K67/0275 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/0306 ,  C07K14/705 ,  C12N15/8509 ,  C12N15/907 ,  C12N2800/107 ,  C12N2800/30

    摘要： Disclosed is a method for introducing a mutation into a gene, which comprises the following steps: a homologous recombination step of carrying out the homologous recombination between a target gene into which the mutation is to be introduced and a target recombinant vector, thereby substituting an exon in the target gene into which the mutation is to be introduced by a target DNA sequence in the target recombinant vector; and a mutation introduction step of carrying out the specific recombination between the target DNA sequence in the resulting target recombinant gene and a mutation introduction cassette of a mutation introduction vector carrying a mutated DNA sequence containing a mutant exon by the intervening action of Cre recombinase to substitute the target DNA sequence by the mutated DNA in the mutation introduction cassette, thereby producing a mutation-introduced gene into which the mutant DNA sequence has been introduced. The method enables the production of a knock-in non-human mammalian animal, such as a knock-in mouse, which carries the mutation-introduced gene.

    摘要翻译： 公开了将突变引入基因的方法，其包括以下步骤：将引入突变的靶基因与靶重组载体进行同源重组的同源重组步骤，从而取代外显子 在目标重组载体中由靶DNA序列引入突变的靶基因中; 以及突变引入步骤，通过Cre重组酶的干预作用来进行所得到的靶重组基因中的靶DNA序列与携带含有突变外显子的突变的DNA序列的突变引入载体的突变导入盒进行替代的特异性重组 通过突变导入盒中的突变DNA的靶DNA序列，从而产生引入突变DNA序列的突变导入基因。 该方法能够产生携带突变导入基因的敲入非人哺乳动物，例如敲入小鼠。