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1.发明申请公开(公告)号：US20110117269A1
公开(公告)日：2011-05-19
申请号：US12930919
申请日：2011-01-20
IPC分类号： B05D5/12
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
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2.发明申请Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US20100243443A1
公开(公告)日：2010-09-30
申请号：US12802573
申请日：2010-06-09
IPC分类号： G01N33/50
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
摘要翻译： 根据本发明的生物传感器包括支撑体1，由诸如金，钯等的诸如贵金属的导电材料构成的导电层2和碳，平行于载体侧的狭缝3a和3b 如图1所示，与支撑体1侧垂直的狭缝4a，4b，工作电极5，对电极6，检测电极7，覆盖工作电极5的间隔件8，对置电极6和检测电极7 支撑体1，形成试样供给路径的矩形切口部9，试料供给路径的入口9a，通过将包含酶等的试剂涂布在工作电极5上而形成的试剂层12，对电极6和 从间隔件8的切口部9暴露的检测电极7和覆盖间隔件8的盖13，如图1所示。 如此构造的生物传感器可以通过简单的方法形成，并且测量精度优异的生物传感器以及试剂层均匀地放置在电极上而不考虑试剂液体组成的生物传感器，并且其中 具有统一的性能可以实现。
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公开(公告)号：US08673127B2
公开(公告)日：2014-03-18
申请号：US12928841
申请日：2010-12-21
申请人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Takahiro Nakaminami , Junko Nakayama , Eriko Yamanishi
发明人： Shoji Miyazaki , Hiroyuki Tokunaga , Masaki Fujiwara , Takahiro Nakaminami , Junko Nakayama , Eriko Yamanishi
IPC分类号： G01N27/327
CPC分类号： C12Q1/003
摘要： In a biosensor for measuring a specific substance in a liquid sample, one or a combination of sugar alcohol, metallic salt, organic acid or organic acid salt which has at least one carboxyl group in a molecule, and organic acid or organic acid salt which has at least one carboxyl group and one amino group in a molecule, is included in a reagent layer provided on electrodes, thereby providing a highly-accurate biosensor which is excellent in stability and has high response (sensitivity, linearity) of the sensor to the substrate concentration.
摘要翻译： 在用于测量液体样品中的特定物质的生物传感器中，分子中具有至少一个羧基的糖醇，金属盐，有机酸或有机酸盐的一种或组合，以及有机酸或有机酸盐，其具有 分子中的至少一个羧基和一个氨基被包含在设置在电极上的试剂层中，从而提供稳定性优异并且具有高传感器对基板的响应（灵敏度，线性）的高精度生物传感器 浓度。
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4.发明授权公开(公告)号：US08480878B2
公开(公告)日：2013-07-09
申请号：US12930627
申请日：2011-01-11
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
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5.发明授权Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US08142629B2
公开(公告)日：2012-03-27
申请号：US12802573
申请日：2010-06-09
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
摘要翻译： 根据本发明的生物传感器包括支撑体1，由诸如金，钯等的诸如贵金属的导电材料构成的导电层2和碳，平行于载体侧的狭缝3a和3b 如图1所示，与支撑体1侧垂直的狭缝4a，4b，工作电极5，对电极6，检测电极7，覆盖工作电极5的间隔件8，对置电极6和检测电极7 支撑体1，形成试样供给路径的矩形切口部9，试料供给路径的入口9a，通过将包含酶等的试剂涂布在工作电极5上而形成的试剂层12，对电极6和 从间隔件8的切口部9暴露的检测电极7和覆盖间隔件8的盖13，如图1所示。 如此构造的生物传感器可以通过简单的方法形成，并且测量精度优异的生物传感器以及试剂层均匀地放置在电极上而不考虑试剂液体组成的生物传感器，并且其中 具有统一的性能可以实现。
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6.发明申请公开(公告)号：US20110272296A1
公开(公告)日：2011-11-10
申请号：US13135816
申请日：2011-07-15
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
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7.发明申请公开(公告)号：US20110147234A1
公开(公告)日：2011-06-23
申请号：US12930957
申请日：2011-01-20
IPC分类号： G01N27/416
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
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8.发明申请BIOSENSOR, THIN FILM ELECTRODE FORMING METHOD, QUANTIFICATION APPARATUS, AND QUANTIFICATION METHOD 有权生物传感器，薄膜电极形成方法，量化装置和定量方法公开(公告)号：US20120251708A1
公开(公告)日：2012-10-04
申请号：US13490553
申请日：2012-06-07
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
摘要翻译： 公开了一种生物传感器，其包括支撑件; 由诸如金或钯等贵金属的导电材料和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。 可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。
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9.发明申请Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US20110278167A1
公开(公告)日：2011-11-17
申请号：US13135848
申请日：2011-07-15
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
摘要翻译： 根据本发明的生物传感器包括支撑体1，由诸如金，钯等的诸如贵金属的导电材料构成的导电层2和碳，平行于载体侧的狭缝3a和3b 如图1所示，与支撑体1侧垂直的狭缝4a，4b，工作电极5，对电极6，检测电极7，覆盖工作电极5的间隔件8，对置电极6和检测电极7 支撑体1，形成试样供给路径的矩形切口部9，试料供给路径的入口9a，通过将包含酶等的试剂涂布在工作电极5上而形成的试剂层12，对电极6和 从间隔件8的切口部9暴露的检测电极7和覆盖间隔件8的盖13，如图1所示。 如此构造的生物传感器可以通过简单的方法形成，并且测量精度优异的生物传感器以及试剂层均匀地放置在电极上而不考虑试剂液体组成的生物传感器，并且其中 具有统一的性能可以实现。
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10.发明授权Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US07998325B2
公开(公告)日：2011-08-16
申请号：US10809217
申请日：2004-03-25
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
摘要翻译： 公开了一种生物传感器，其包括支撑件; 由诸如金或钯等贵金属的导电材料和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。 可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。
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