Apparatus for separating living cells
    8.
    发明授权
    Apparatus for separating living cells 失效
    用于分离活细胞的装置

    公开(公告)号:US5227066A

    公开(公告)日:1993-07-13

    申请号:US722219

    申请日:1991-06-27

    摘要: A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotatable in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged form the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.

    摘要翻译: 公开了一种用于活细胞的离心分离方法,其中将含细胞的溶液装入由耐蒸汽压壁限制的转子室中。 具有隔片的转子设置在转子室中。 隔垫可以以与转子相同的角速度在相同的方向上旋转,而当细胞从含细胞溶液中离心时,其至少部分浸入到转子的溶液中。 在细胞分离并粘附到转子的内表面之后,转子中的上清液从转子排出。 然后将新鲜的培养介质引入转子中,并且隔片和转子以相互不同的角速度旋转,同时至少部分隔片浸入转子中的新鲜介质中。 结果,细胞再次悬浮在新鲜培养基中。

    Culturing method, system and apparatus for cell culture
    9.
    发明授权
    Culturing method, system and apparatus for cell culture 失效
    培养方法,细胞培养的系统和装置

    公开(公告)号:US5166067A

    公开(公告)日:1992-11-24

    申请号:US443108

    申请日:1989-11-29

    IPC分类号: C12M3/06 C12N5/02 C12N5/06

    CPC分类号: C12M29/16 C12M29/02

    摘要: A culturing method and system are disclosed in which a cell culture broth in withdrawn from a culturing apparatus to a filteration apparatus. The withdrawn broth in brought into contact with a membrane for precision filteration. Then a non-toxic gas is brown into the broth, while filtering the broth to separate the cells and breaking foams occurring on the surface of the broth due to gas bubbling. The separated cells are returned to the vessel and the filterate is withdrawn from the filteration apparatus.The method and system have such advantages that a perfusion culture of a long period of time can be realized because the clogging of the filteration membrane is effectively prevented.

    Method of separating living cells
    10.
    发明授权
    Method of separating living cells 失效
    分离活细胞的方法

    公开(公告)号:US5030361A

    公开(公告)日:1991-07-09

    申请号:US431237

    申请日:1989-11-03

    摘要: A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotatable in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged from the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.