公开(公告)号：US20080248525A1

公开(公告)日：2008-10-09

申请号：US11809756

申请日：2007-06-01

申请人： Sierd Bron ,  Jan D.H. Jongbloed ,  Joerg Muller ,  Jan M. Van Dijl

发明人： Sierd Bron ,  Jan D.H. Jongbloed ,  Joerg Muller ,  Jan M. Van Dijl

IPC分类号： C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N1/20

CPC分类号： C07K14/32 ,  C07K2319/034 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75 ,  C12P21/02

摘要： Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coil or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

摘要翻译： 本文描述了增强宿主细胞中蛋白质分泌的方法。 在优选的实施方案中，宿主细胞是革兰氏阳性微生物如芽孢杆菌。 在另一个优选的实施方案中，宿主细胞是革兰氏阴性微生物。 优选地，革兰氏阴性微生物是大肠杆菌或Pantoaea属的成员。 蛋白质分泌可能通过Tat途径的蛋白质组分的过度表达来增强。 或者，通过形成包含tat信号序列和感兴趣的蛋白质的嵌合多肽，可以选择性地增强外来蛋白质的分泌。 在优选的实施方案中，tat信号序列选自phoD或LipA。

公开(公告)号：US20090093025A1

公开(公告)日：2009-04-09

申请号：US11809528

申请日：2007-06-01

申请人： Sierd Bron ,  Jan D.H. Jongbloed ,  Joerg Muller ,  Jan M. Van Dijl

发明人： Sierd Bron ,  Jan D.H. Jongbloed ,  Joerg Muller ,  Jan M. Van Dijl

IPC分类号： C12P21/02 ,  C07K19/00 ,  C07H21/00 ,  C12N1/21

CPC分类号： C07K14/32 ,  C07K2319/034 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75 ,  C12P21/02

摘要： Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

公开(公告)号：US20080166757A1

公开(公告)日：2008-07-10

申请号：US11809669

申请日：2007-06-01

申请人： Sierd Bron ,  Jan D.H. Jongbloed ,  Joerg Muller ,  Jan M. Van Dijl

发明人： Sierd Bron ,  Jan D.H. Jongbloed ,  Joerg Muller ,  Jan M. Van Dijl

IPC分类号： C12P21/04 ,  C12N9/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06

CPC分类号： C07K14/32 ,  C07K2319/034 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75 ,  C12P21/02

摘要： Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

摘要翻译： 本文描述了增强宿主细胞中蛋白质分泌的方法。 在优选的实施方案中，宿主细胞是革兰氏阳性微生物如芽孢杆菌。 在另一个优选的实施方案中，宿主细胞是革兰氏阴性微生物。 优选地，革兰氏阴性微生物是大肠杆菌或Pantoaea属的成员。 蛋白质分泌可能通过Tat途径的蛋白质组分的过度表达来增强。 或者，通过形成包含tat信号序列和感兴趣的蛋白质的嵌合多肽，可以选择性地增强外来蛋白质的分泌。 在优选的实施方案中，tat信号序列选自phoD或LipA。