公开(公告)号：US08377681B2

公开(公告)日：2013-02-19

申请号：US13310332

申请日：2011-12-02

申请人： Stephen DelCardayre ,  Matthew B. Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy S. Minshull ,  Phillip A. Patten ,  Venkiteswatan Mani Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Anthony R. Cox ,  Gjalt W. Huisman ,  Ling Yuan ,  Joseph A. Affholter

发明人： Stephen DelCardayre ,  Matthew B. Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy S. Minshull ,  Phillip A. Patten ,  Venkiteswatan Mani Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Anthony R. Cox ,  Gjalt W. Huisman ,  Ling Yuan ,  Joseph A. Affholter

IPC分类号： C12N1/16 ,  C12N1/18

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US07629170B2

公开(公告)日：2009-12-08

申请号：US11506215

申请日：2006-08-17

申请人： Stephen delCardayre ,  Matthew B. Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy S. Minshull ,  Phillip A. Patten ,  Venkiteswatan Mani Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Anthony R. Cox ,  Gjalt W. Huisman ,  Ling Yuan ,  Joseph A. Affholter

发明人： Stephen delCardayre ,  Matthew B. Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy S. Minshull ,  Phillip A. Patten ,  Venkiteswatan Mani Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Anthony R. Cox ,  Gjalt W. Huisman ,  Ling Yuan ,  Joseph A. Affholter

IPC分类号： C12N15/00 ,  C12N15/87 ,  C12N15/63

CPC分类号： C12R1/465 ,  A01K2267/02 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/90 ,  C12P17/162 ,  C12Q1/6811 ,  C12Q1/6827

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US08076138B2

公开(公告)日：2011-12-13

申请号：US12580056

申请日：2009-10-15

申请人： Stephen DelCardayre ,  Matthew B. Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy S. Minshull ,  Phillip A. Patten ,  Venkiteswatan Mani Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Anthony R. Cox ,  Gjalt W. Huisman ,  Ling Yuan ,  Joseph A. Affholter

发明人： Stephen DelCardayre ,  Matthew B. Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy S. Minshull ,  Phillip A. Patten ,  Venkiteswatan Mani Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Anthony R. Cox ,  Gjalt W. Huisman ,  Ling Yuan ,  Joseph A. Affholter

IPC分类号： C12N15/00 ,  C12N15/87 ,  C12N15/63

CPC分类号： C12R1/465 ,  A01K2267/02 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/90 ,  C12P17/162 ,  C12Q1/6811 ,  C12Q1/6827

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

摘要翻译： 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。

公开(公告)号：US07148054B2

公开(公告)日：2006-12-12

申请号：US10194686

申请日：2002-07-11

申请人： Stephen delCardayre ,  Matthew Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steve Bass ,  Ying-Xin Zhang ,  Tony Cox ,  Gjalt Huisman ,  Ling Yuan ,  Joseph A. Affholter

发明人： Stephen delCardayre ,  Matthew Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steve Bass ,  Ying-Xin Zhang ,  Tony Cox ,  Gjalt Huisman ,  Ling Yuan ,  Joseph A. Affholter

IPC分类号： C12N1/16 ,  C12N1/18

CPC分类号： C12R1/465 ,  A01K2267/02 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/90 ,  C12P17/162 ,  C12Q1/6811 ,  C12Q1/6827

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

摘要翻译： 本发明提供了使用重组和选择/筛选整个细胞和生物体以获得所需性质的进化的迭代循环的方法。这些性质的实例包括增加的重组原型，基因组拷贝数和蛋白质和次级代谢物的表达和/或分泌能力 。

公开(公告)号：US20120252681A1

公开(公告)日：2012-10-04

申请号：US13310332

申请日：2011-12-02

申请人： Stephen del Cardayre ,  Matthew Tobin ,  Willem P.C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Tony Cox ,  Gjalt Huisman ,  Ling Yuan ,  Joseph A. Affholter

发明人： Stephen del Cardayre ,  Matthew Tobin ,  Willem P.C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Tony Cox ,  Gjalt Huisman ,  Ling Yuan ,  Joseph A. Affholter

IPC分类号： C40B10/00

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

摘要翻译： 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。

公开(公告)号：US20110190140A1

公开(公告)日：2011-08-04

申请号：US12580056

申请日：2009-10-15

申请人： Stephen del Cardayre ,  Matthew Tobin ,  Willem P.C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Tony Cox ,  Gjalt Huisman ,  Ling Yuan ,  Joseph A. Affholter

发明人： Stephen del Cardayre ,  Matthew Tobin ,  Willem P.C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda A. Castle ,  Claus M. Krebber ,  Steven H. Bass ,  Ying-Xin Zhang ,  Tony Cox ,  Gjalt Huisman ,  Ling Yuan ,  Joseph A. Affholter

IPC分类号： C40B10/00 ,  C12N15/04

CPC分类号： C12R1/465 ,  A01K2267/02 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12N15/90 ,  C12P17/162 ,  C12Q1/6811 ,  C12Q1/6827

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

摘要翻译： 本发明提供了采用迭代循环重组和选择/筛选全细胞和生物体以获得所需性质的方法的方法。 这些性质的实例包括增加的重组原性，基因组拷贝数以及蛋白质和次级代谢物的表达和/或分泌能力。

公开(公告)号：US06287862B1

公开(公告)日：2001-09-11

申请号：US09626410

申请日：2000-07-26

申请人： Stephen delCardayre ,  Matthew Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steven H. Bass

发明人： Stephen delCardayre ,  Matthew Tobin ,  Willem P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steven H. Bass

IPC分类号： C12N1500

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US06528311B1

公开(公告)日：2003-03-04

申请号：US09516051

申请日：2000-03-01

申请人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

发明人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswaran Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

IPC分类号： C12N1500

CPC分类号： C12N15/102 ,  C07K14/245 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12P17/162 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6827 ,  C12R1/465

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US06352859B1

公开(公告)日：2002-03-05

申请号：US09626343

申请日：2000-07-26

申请人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

发明人： Stephen delCardayre ,  Matthew Tobin ,  William P. C. Stemmer ,  Jon E. Ness ,  Jeremy Minshull ,  Phillip Patten ,  Venkiteswatan Subramanian ,  Linda Castle ,  Claus M. Krebber ,  Steve Bass

IPC分类号： C12N1500

CPC分类号： A01H1/00 ,  A01K2217/05 ,  C12N15/1027 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8279 ,  C12Q1/6811

摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

公开(公告)号：US06337186B1

公开(公告)日：2002-01-08

申请号：US09333762

申请日：1999-06-15

申请人： Claus M. Krebber

发明人： Claus M. Krebber

IPC分类号： C12Q168

CPC分类号： C12N15/1027 ,  C12N15/102

摘要： The invention provides methods for the production of polynucleotides with a desired property (e.g., conferring a desired phenotype and/or encoding polypeptide with a desired property) which is selectable or can be screened for. The method includes making insertions and/or deletions at random sites in DNA segments in a population. In some embodiments the random insertions and deletions are made recursively.

摘要翻译： 本发明提供了可选择或可筛选的具有所需性质（例如赋予所需表型和/或编码具有所需性质的多肽）的多核苷酸的方法。 该方法包括在群体中的DNA片段的随机位点进行插入和/或缺失。 在一些实施例中，递归地进行随机插入和缺失。