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公开(公告)号：US20090246766A1
公开(公告)日：2009-10-01
申请号：US12156432
申请日：2008-05-30
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。
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公开(公告)号：US08669059B2
公开(公告)日：2014-03-11
申请号：US13585758
申请日：2012-08-14
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。
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3.发明申请公开(公告)号：US20120309014A1
公开(公告)日：2012-12-06
申请号：US13585758
申请日：2012-08-14
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。
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公开(公告)号：US08268553B2
公开(公告)日：2012-09-18
申请号：US12156432
申请日：2008-05-30
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Kari Mikkelson , Yan Poon
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。
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5.发明授权公开(公告)号：US08318436B2
公开(公告)日：2012-11-27
申请号：US13269414
申请日：2011-10-07
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
IPC分类号： C12Q1/68 , C12Q1/34 , C12P19/30 , C12N15/64 , C12N9/42 , C12N1/00 , C12N1/12 , C07H21/02 , C07H19/04
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。
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公开(公告)号：US20120107853A1
公开(公告)日：2012-05-03
申请号：US13269414
申请日：2011-10-07
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
IPC分类号： C12Q1/34
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。
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公开(公告)号：US20090280545A1
公开(公告)日：2009-11-12
申请号：US12207761
申请日：2008-09-10
申请人： Michael Mendez , Stephen Mayfield , Bryan O'Neill , Yan Poon , Phillip Lee , Craig Aaron Behnke , Su-Chiung Fang
发明人： Michael Mendez , Stephen Mayfield , Bryan O'Neill , Yan Poon , Phillip Lee , Craig Aaron Behnke , Su-Chiung Fang
CPC分类号： C12P23/00 , C12N15/902 , C12P7/04 , C12P9/00
摘要： The present invention provides compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of products. The methods and compositions are particularly useful in the petrochemical industry.
摘要翻译： 本发明提供了通过光合生物产生产物的组合物和方法。 光合生物被遗传修饰以产生产物，分泌物或两者的产物。 所述方法和组合物在石化工业中特别有用。
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公开(公告)号：US08143039B2
公开(公告)日：2012-03-27
申请号：US12156450
申请日：2008-05-30
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
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9.发明申请公开(公告)号：US20090253169A1
公开(公告)日：2009-10-08
申请号：US12156450
申请日：2008-05-30
申请人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
发明人： Stephen Mayfield , Bryan O'Neill , Michael Mendez , Yan Poon
CPC分类号： C12Q1/6895 , C12N15/8214 , Y02E50/16
摘要： The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
摘要翻译： 本发明提供了一种用于高通量筛选用于等离子体状态的遗传修饰的光合生物的方法和组合物。 本发明提供了在植物中产生一种或多种蛋白质，包括生物降解酶的方法。 还提供了在藻细胞，特别是在叶绿体中生产生物质降解途径的方法。 可以通过所公开的方法产生单一酶或多种酶。 本文公开的方法允许生产生物燃料，包括乙醇。
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10.发明授权System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts 有权用于捕获和修饰大片基因组DNA并用叶绿体构建生物体的系统公开(公告)号：US08314222B2
公开(公告)日：2012-11-20
申请号：US12287230
申请日：2008-10-06
申请人： Michael Mendez , Bryan O'Neill , Kari Mikkelson
发明人： Michael Mendez , Bryan O'Neill , Kari Mikkelson
CPC分类号： C12N15/79 , C12N15/81 , C12N15/82 , C12N15/8214 , C12N2800/204 , C12N2800/206
摘要： The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.
摘要翻译： 基因的功能分析通常需要操纵大的基因组区域。 描述了可用于通过同源重组克隆DNA的大区域的酵母细菌穿梭载体。 还描述了用于分离整个基因组（包括叶绿体基因组）或其大部分并进行操作的方法。 还描述了确定最小基因组，最小途径要求和最小细胞器基因组的方法。
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