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1.
公开(公告)号:US08906645B2
公开(公告)日:2014-12-09
申请号:US13809214
申请日:2011-12-27
申请人: Steven P. Swanson , Jesse D. Miller , Clinton P. Waller, Jr. , Neil Percy , Jeffrey A. Lucas , Kannan Seshadri , Jerald K. Rasmussen , Douglas E. Weiss , James E. Aysta
发明人: Steven P. Swanson , Jesse D. Miller , Clinton P. Waller, Jr. , Neil Percy , Jeffrey A. Lucas , Kannan Seshadri , Jerald K. Rasmussen , Douglas E. Weiss , James E. Aysta
摘要: A microbial detection article and methods of using the same, the article comprising: a base member comprising a self-supporting water impervious substrate with first and second generally opposed major surfaces; a filter assembly defining a filter assembly aperture therein, and having a composite filter body mounted across the filter assembly aperture; wherein the composite filter body comprises: a microporous membrane, and a water-absorbent layer in fluid communication with the microporous membrane; and a cover sheet.
摘要翻译: 一种微生物检测制品及其使用方法,所述制品包括:基底构件,其包括具有第一和第二大致相对的主表面的自支撑防水基底; 过滤器组件,其中限定过滤器组件孔,并且具有穿过过滤器组件孔安装的复合过滤器主体; 其中所述复合过滤器主体包括:微多孔膜和与所述微多孔膜流体连通的吸水层; 和封面片。
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2.
公开(公告)号:US20130316393A1
公开(公告)日:2013-11-28
申请号:US13809214
申请日:2011-12-27
申请人: Steven P. Swanson , Jesse D. Miller , Clinton P. Waller, JR. , Neil Percy , Jeffrey A. Lucas , Kannan Seshadri , Jerald K. Rasmussen , Douglas E. Weiss , James E. Aysta
发明人: Steven P. Swanson , Jesse D. Miller , Clinton P. Waller, JR. , Neil Percy , Jeffrey A. Lucas , Kannan Seshadri , Jerald K. Rasmussen , Douglas E. Weiss , James E. Aysta
IPC分类号: C12Q1/04
摘要: A microbial detection article and methods of using the same, the article comprising: a base member comprising a self-supporting water impervious substrate with first and second generally opposed major surfaces; a filter assembly defining a filter assembly aperture therein, and having a composite filter body mounted across the filter assembly aperture; wherein the composite filter body comprises: a microporous membrane, and a water-absorbent layer in fluid communication with the microporous membrane; and a cover sheet.
摘要翻译: 一种微生物检测制品及其使用方法,所述制品包括:基底构件,其包括具有第一和第二大致相对的主表面的自支撑防水基底; 过滤器组件,其中限定过滤器组件孔,并且具有穿过过滤器组件孔安装的复合过滤器主体; 其中所述复合过滤器主体包括:微多孔膜和与所述微多孔膜流体连通的吸水层; 和封面片。
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公开(公告)号:US08846335B2
公开(公告)日:2014-09-30
申请号:US13703149
申请日:2011-06-27
CPC分类号: B01L9/52 , B82Y5/00 , C12M23/20 , C12M23/22 , C12M25/06 , C12M41/36 , C12Q1/06 , Y10T428/259
摘要: The disclosure includes: a coating composition, comprising a powdered cold-water-soluble gelling agent and surface-modified nanoparticles disposed in the powdered cold-water-soluble gelling agent; a coated film that includes a transparent film coated with the coating composition; and a device for growing microorganisms, including the coated film releasably attached to at least a portion of a body member that includes a self-supporting and water-proof substrate.
摘要翻译: 本发明包括:涂料组合物,其包含粉末状冷水溶性胶凝剂和设置在粉末状冷水溶性胶凝剂中的表面改性纳米颗粒; 包括涂覆有所述涂料组合物的透明膜的涂膜; 以及用于生长微生物的装置,包括可释放地附着到包括自支撑和防水衬底的主体构件的至少一部分上的涂膜。
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公开(公告)号:US20130089923A1
公开(公告)日:2013-04-11
申请号:US13703149
申请日:2011-06-27
IPC分类号: C12M1/00
CPC分类号: B01L9/52 , B82Y5/00 , C12M23/20 , C12M23/22 , C12M25/06 , C12M41/36 , C12Q1/06 , Y10T428/259
摘要: The disclosure includes: a coating composition, comprising a powdered cold-water-soluble gelling agent and surface-modified nanoparticles disposed in the powdered cold-water-soluble gelling agent; a coated film that includes a transparent film coated with the coating composition; and a device for growing microorganisms, including the coated film releasably attached to at least a portion of a body member that includes a self-supporting and water-proof substrate.
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公开(公告)号:US20100203537A1
公开(公告)日:2010-08-12
申请号:US12680049
申请日:2008-09-26
申请人: Jesse D. Miller
发明人: Jesse D. Miller
CPC分类号: C12Q1/686 , C12Q1/6818 , C12Q1/6853 , C12Q2563/107 , C12Q2531/113 , C12Q2527/107 , C12Q2525/161
摘要: Methods for amplifying and detecting nucleic acids are described, as well as sets of 5′ labeled oligonucleotides.
摘要翻译: 描述了用于扩增和检测核酸的方法,以及5'标记寡核苷酸的组。
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公开(公告)号:US08753834B2
公开(公告)日:2014-06-17
申请号:US13519284
申请日:2010-12-30
IPC分类号: C12Q1/04
CPC分类号: G01N33/56911 , C12M23/20 , C12M25/02 , C12M41/36 , C12Q1/04 , C12Q1/06 , G01N33/569
摘要: The disclosure provides an article for the detection of a microorganism in a liquid sample. The article comprises a microporous membrane and a barrier layer to selectively regulate the contact between the sample and a detection reagent. A method of use is also provided.
摘要翻译: 本公开提供了用于检测液体样品中的微生物的制品。 该制品包括微孔膜和阻挡层,以选择性地调节样品与检测试剂之间的接触。 还提供了一种使用方法。
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公开(公告)号:US20130230876A1
公开(公告)日:2013-09-05
申请号:US13883620
申请日:2011-10-28
申请人: Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
发明人: Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
IPC分类号: G01N21/64
CPC分类号: G01N21/6486 , C12Q1/04
摘要: Compositions that comprise water, a first indicator reagent that can be converted by a first biological activity to a first biological derivative, and a plurality of particles are provided. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore selected from the group consisting of umbelliferone, 7-aminocoumarin, β-naphthylamine, β-naphthol, fluorescein, resorufin, 9H-(1,3-dichloro-9,9-dimethyl acridin-2-one), rhodamine 110, a derivative of any of the foregoing fluorophores, and a combination of any of the foregoing fluorophores. The particles are capable of receiving and retaining the first biological derivative from an aqueous liquid. The first biological derivative can be indicative of a microorganism. The compositions further can comprise a gelling agent. Methods of using the compositions to detect the presence or absence of a microorganism in a sample are also provided.
摘要翻译: 提供了包含水,可以通过第一生物活性转化为第一生物衍生物的第一指示剂试剂和多个颗粒的组合物。 第一指示剂可以包括具有选自伞形酮,7-氨基香豆素,β-萘胺,β-萘酚,荧光素,再次吸收的9H-(1,3-二氯-9,9-四氢 - 二甲基吖啶-2-酮),罗丹明110,任何前述荧光团的衍生物,以及任何上述荧光团的组合。 颗粒能够从水性液体中接收和保留第一生物衍生物。 第一种生物衍生物可以指示微生物。 组合物还可以包含胶凝剂。 还提供了使用组合物来检测样品中微生物的存在或不存在的方法。
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公开(公告)号:US09435739B2
公开(公告)日:2016-09-06
申请号:US13883620
申请日:2011-10-28
申请人: Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
发明人: Stephen B. Roscoe , Kurt J. Halverson , Jesse D. Miller , Stephanie J. Moeller , Jason W. Bjork
CPC分类号: G01N21/6486 , C12Q1/04
摘要: Compositions that comprise water, a first indicator reagent that can be converted by a first biological activity to a first biological derivative, and a plurality of particles are provided. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore selected from the group consisting of umbelliferone, 7-aminocoumarin, β-naphthylamine, β-naphthol, fluorescein, resorufin, 9H-(1,3-dichloro-9,9-dimethyl acridin-2-one), rhodamine 110, a derivative of any of the foregoing fluorophores, and a combination of any of the foregoing fluorophores. The particles are capable of receiving and retaining the first biological derivative from an aqueous liquid. The first biological derivative can be indicative of a microorganism. The compositions further can comprise a gelling agent. Methods of using the compositions to detect the presence or absence of a microorganism in a sample are also provided.
摘要翻译: 提供了包含水,可以通过第一生物活性转化为第一生物衍生物的第一指示剂试剂和多个颗粒的组合物。 第一指示剂可以包含具有选自伞形酮,7-氨基香豆素,β-萘胺,β-萘酚,荧光素,再利用者,9H-(1,3-二氯-9,9-四氢 - 二甲基吖啶-2-酮),罗丹明110,任何前述荧光团的衍生物,以及任何上述荧光团的组合。 颗粒能够从水性液体中接收和保留第一生物衍生物。 第一种生物衍生物可以指示微生物。 组合物还可以包含胶凝剂。 还提供了使用组合物来检测样品中微生物的存在或不存在的方法。
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公开(公告)号:US20120288888A1
公开(公告)日:2012-11-15
申请号:US13519284
申请日:2010-12-30
CPC分类号: G01N33/56911 , C12M23/20 , C12M25/02 , C12M41/36 , C12Q1/04 , C12Q1/06 , G01N33/569
摘要: The disclosure provides an article for the detection of a microorganism in a liquid sample. The article comprises a microporous membrane and a barrier layer to selectively regulate the contact between the sample and a detection reagent. A method of use is also provided.
摘要翻译: 本公开提供了用于检测液体样品中的微生物的制品。 该制品包括微孔膜和阻挡层,以选择性地调节样品与检测试剂之间的接触。 还提供了一种使用方法。
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公开(公告)号:US09434975B2
公开(公告)日:2016-09-06
申请号:US14008905
申请日:2012-03-05
申请人: Stephen B. Roscoe , Shih-Hung Chou , Stephanie J. Moeller , Jesse D. Miller , Kurt J. Halverson , Jason W. Bjork
发明人: Stephen B. Roscoe , Shih-Hung Chou , Stephanie J. Moeller , Jesse D. Miller , Kurt J. Halverson , Jason W. Bjork
IPC分类号: C12Q1/04 , C07D311/16 , C07D405/04 , C07D409/04 , C07H17/075 , G01N33/52 , G01N33/58
CPC分类号: C12Q1/04 , C07D311/16 , C07D405/04 , C07D409/04 , C07H17/075 , C09B57/02 , C12Q1/045 , G01N33/525 , G01N33/582
摘要: Compounds are provided that are either fluorogenic or fluorophoric. Compositions and articles that include the compounds are also provided. Additionally, methods of detecting a microorganism using the compounds are provided. The compounds are fluorinated and can be used advantageously under acidic conditions.
摘要翻译: 提供了荧光或荧光的化合物。 还提供了包含化合物的组合物和制品。 另外,提供使用该化合物检测微生物的方法。 化合物是氟化的,并且可以在酸性条件下有利地使用。
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