公开(公告)号：US09151682B2

公开(公告)日：2015-10-06

申请号：US13696893

申请日：2010-12-08

申请人： Sun Kyu Lee ,  Sung Ki Nam ,  Su Heon Jeong ,  Jung Kyun Kim ,  Do Kyun Woo ,  Sung Yang

发明人： Sun Kyu Lee ,  Sung Ki Nam ,  Su Heon Jeong ,  Jung Kyun Kim ,  Do Kyun Woo ,  Sung Yang

IPC分类号： G01K17/00 ,  B01L3/00 ,  G01N25/20 ,  G01N25/48 ,  C12Q1/68

CPC分类号： G01K17/00 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/1827 ,  C12Q1/6827 ,  G01K17/006 ,  G01N25/20 ,  G01N25/4846 ,  G01N25/4866 ,  G01N25/4893

摘要： A sensor for measuring heat generated from cells, including a thermopile manufactured by surface-micromachining technology, and a microfluidic flow-channel structure for mixing cells with medium and dividing the mixture into cells and medium. Medium and cells are uniformly mixed using a micro-mixer. The mixture is separated into a medium microfluid and a cell microfluid using the Zweifach-Fung effect, after which signals measured in the two microfluids are amplified. The difference between the two signal values determines the amount of heat generated from the cells. The influence of noise caused by a change in external environment is eliminated. Convection heat dissipation caused by fluid flow is minimized. The sensor accurately measures the amount of heat generated from cells flowing in the microfluidic flow-channel. Diseases such as cancer are diagnosed using the difference between the measured amount of heat generated from cells and the standard amount of heat generated from normal cells.

摘要翻译： 用于测量由细胞产生的热量的传感器，包括通过表面微加工技术制造的热电堆，以及用于将细胞与培养基混合并将混合物分成细胞和培养基的微流体流动通道结构。 使用微型混合器将培养基和细胞均匀混合。 使用Zweifach-Fung效应将混合物分离成介质微流体和细胞微流体，之后扩增在两个微流体中测量的信号。 两个信号值之间的差异决定了从单元格产生的热量。 消除了由外部环境变化引起的噪声的影响。 由流体流动引起的对流散热最小化。 传感器精确地测量在微流体流动通道中流动的细胞产生的热量。 使用从细胞产生的测量量与从正常细胞产生的标准热量之间的差异来诊断诸如癌症的疾病。

公开(公告)号：US20130064265A1

公开(公告)日：2013-03-14

申请号：US13696893

申请日：2010-12-08

申请人： Sun Kyu Lee ,  Sung Ki Nam ,  Su Heon Jeong ,  Jung Kyun Kim ,  Do Kyun Woo ,  Sung Yang

发明人： Sun Kyu Lee ,  Sung Ki Nam ,  Su Heon Jeong ,  Jung Kyun Kim ,  Do Kyun Woo ,  Sung Yang

IPC分类号： G01K17/00

CPC分类号： G01K17/00 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/1827 ,  C12Q1/6827 ,  G01K17/006 ,  G01N25/20 ,  G01N25/4846 ,  G01N25/4866 ,  G01N25/4893

摘要： A sensor for measuring heat generated from cells, including a thermopile manufactured by surface-micromachining technology, and a microfluidic flow-channel structure for mixing cells with medium and dividing the mixture into cells and medium. Medium and cells are uniformly mixed using a micro-mixer. The mixture is separated into a medium microfluid and a cell microfluid using the Zweifach-Fung effect, after which signals measured in the two microfluids are amplified. The difference between the two signal values determines the amount of heat generated from the cells. The influence of noise caused by a change in external environment is eliminated. Convection heat dissipation caused by fluid flow is minimized. The sensor accurately measures the amount of heat generated from cells flowing in the microfluidic flow-channel. Diseases such as cancer are diagnosed using the difference between the measured amount of heat generated from cells and the standard amount of heat generated from normal cells.

摘要翻译： 用于测量由细胞产生的热量的传感器，包括通过表面微加工技术制造的热电堆，以及用于将细胞与培养基混合并将混合物分成细胞和培养基的微流体流动通道结构。 使用微型混合器将培养基和细胞均匀混合。 使用Zweifach-Fung效应将混合物分离成介质微流体和细胞微流体，之后扩增在两个微流体中测量的信号。 两个信号值之间的差异决定了从单元格产生的热量。 消除了由外部环境变化引起的噪声的影响。 由流体流动引起的对流散热最小化。 传感器精确地测量在微流体流动通道中流动的细胞产生的热量。 使用从细胞产生的测量量与从正常细胞产生的标准热量之间的差异来诊断诸如癌症的疾病。