公开(公告)号：US20160115488A1

公开(公告)日：2016-04-28

申请号：US14990444

申请日：2016-01-07

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE ROCKEFELLER UNIVERSITY

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Luciano Marraffini ,  David Olivier Bikard ,  Wenyan Jiang ,  Neville Espi Sanjana

IPC分类号： C12N15/74 ,  C12N15/70

CPC分类号： C12N15/746 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/907 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/101

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

公开(公告)号：US20160115489A1

公开(公告)日：2016-04-28

申请号：US14991083

申请日：2016-01-08

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE ROCKEFELLER UNIVERSITY

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Luciano Marraffini ,  David Olivier Bikard ,  Wenyan Jiang ,  Neville Espi Sanjana

IPC分类号： C12N15/74 ,  C12N15/70

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

公开(公告)号：US09822372B2

公开(公告)日：2017-11-21

申请号：US14990444

申请日：2016-01-07

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE ROCKEFELLER UNIVERSITY

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Luciano Marraffini ,  David Olivier Bikard ,  Wenyan Jiang ,  Neville Espi Sanjana

IPC分类号： C12Q1/02 ,  C12N15/74 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C12N15/70 ,  C12N15/90 ,  C12N15/113 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04 ,  C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/746 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/907 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/101

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

公开(公告)号：US20220135985A1

公开(公告)日：2022-05-05

申请号：US17503928

申请日：2021-10-18

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  The Rockefeller University

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Luciano Marraffini ,  David Olivier Bikard ,  Wenyan Jiang ,  Neville Espi Sanjana

IPC分类号： C12N15/74 ,  C12N15/85 ,  C12N15/63 ,  C12N15/90 ,  C12N15/113 ,  C12N15/70 ,  C12N9/22 ,  C12N15/10

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

公开(公告)号：US20180179547A1

公开(公告)日：2018-06-28

申请号：US15887377

申请日：2018-02-02

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE ROCKEFELLER UNIVERSITY

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Luciano Marraffini ,  David Olivier Bikard ,  Wenyan Jiang ,  Neville Espi Sanjana

IPC分类号： C12N15/74 ,  C12N15/90 ,  C12N9/22 ,  C12N15/85 ,  C12N15/70 ,  C12N15/63

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

公开(公告)号：US20150031134A1

公开(公告)日：2015-01-29

申请号：US14497627

申请日：2014-09-26

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE ROCKEFELLER UNIVERSITY

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Luciano Marraffini ,  David Olivier Bikard ,  Wenyan Jiang

IPC分类号： C12N15/70

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

摘要翻译： 本发明提供了用于操纵靶序列的序列和/或活性的系统，方法和组合物。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及用于设计和使用这种载体的方法。 还提供了在真核细胞中引导CRISPR复合物形成的方法以及通过引入使用CRISPR / Cas系统的精确突变来选择特异性细胞的方法。