-
公开(公告)号：US20210388426A1
公开(公告)日：2021-12-16
申请号：US17416399
申请日：2019-12-20
Applicant: The Johns Hopkins University
Inventor： Tza-Huei Jeff WANG , Ye ZHANG
IPC: C12Q1/6844 , G01N21/64 , B01L3/00 , G02B21/00
Abstract: Methods for multiplexed detection of a nucleic acid sequence in a sample including the use of a plurality of oligonucleotide target-specific probes (TSPs) configured to bind to a distinct target nucleic acid sequence, where each of the TSPs includes one or more copies of a first fluorescent probe (FP) binding region and one or more copies of a second FP binding region, and where a predetermined ratio of the one or more copies of the first FP binding region to the one or more copies of the second FP binding region is indicative of the distinct target nucleic acid sequence the TSP is configured to bind to.
-
公开(公告)号：US20240271226A1
公开(公告)日：2024-08-15
申请号：US18245048
申请日：2021-09-29
Applicant: THE JOHNS HOPKINS UNIVERSITY
Inventor： Tza-Huei Jeff WANG , Aniruddha Mrithinjay KAUSHIK , Kuangwen HSIEH
IPC: C12Q1/689 , B01L3/00 , C12Q1/6818 , C12Q1/6897
CPC classification number: C12Q1/689 , B01L3/502784 , C12Q1/6818 , C12Q1/6897 , B01L2200/0673 , B01L2200/16 , B01L2300/0663 , B01L2300/0829 , B01L2300/0883
Abstract: Provided herein are methods of identifying and determining the antimicrobial susceptibility of bacteria in samples. Related devices, systems, reaction mixtures, kits, and other methods are also provided.
-
3.发明公开
公开(公告)号：US20240123447A1
公开(公告)日：2024-04-18
申请号：US18263010
申请日：2022-01-28
Applicant: THE JOHNS HOPKINS UNIVERSITY
Inventor： Tza-Huei Jeff WANG , Alexander Y. TRICK , Fan-En CHEN , Liben CHEN
IPC: B01L3/00 , G01N33/543
CPC classification number: B01L3/502761 , G01N33/54326 , B01L2200/027 , B01L2200/0647 , B01L2200/0689 , B01L2200/10 , B01L2200/16 , B01L2300/12 , B01L2400/043
Abstract: Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells for performing controlled serial elution techniques that enables execution of extraction/purification and splitting of analytes for multiplex detection via magnetic actuation only. Related magnetofluidic devices, kits, and methods are also provided.
-
公开(公告)号：US20230201833A1
公开(公告)日：2023-06-29
申请号：US17999677
申请日：2021-05-27
Applicant: THE JOHNS HOPKINS UNIVERSITY
Inventor： Tza-Huei Jeff WANG , Alexander Y. TRICK , Fan-En CHEN
CPC classification number: B01L3/502761 , B01L3/502715 , G01N35/0098 , B01L2200/0647 , B01L2200/0684 , B01L2300/0816 , B01L2300/087 , B01L2300/0874 , B01L2300/1805 , B01L2400/043 , B01L2200/0689 , B01L2200/16
Abstract: Provided herein are magnetofluidic cartridges of use in a wide variety of sample analysis applications, including nucleic acid amplification assays. The magnetofluidic cartridges include sample inlet wells and sample analysis wells. Temperature sensitive materials are used to separate the sample inlet wells and sample analysis wells from one another prior to performing a given sample analysis application. Related magnetofluidic devices, kits, and methods are also provided.
-
公开(公告)号：US20240368710A1
公开(公告)日：2024-11-07
申请号：US18772429
申请日：2024-07-15
Applicant: THE JOHNS HOPKINS UNIVERSITY
Inventor： Tza-Huei Jeff WANG , Pornpat ATHAMANOLAP , Liben CHEN , Kuangwen HSIEH
IPC: C12Q1/689 , B01L3/00 , C12Q1/06 , C12Q1/6851 , C12Q1/686
Abstract: Described are methods for identifying antibiotic resistant bacteria, quantifying bacteria growth, and applying an antibiotic susceptibility test (AST) in one or more biological samples containing a bacteria and chips used in these methods.
-
公开(公告)号：US20220362772A1
公开(公告)日：2022-11-17
申请号：US17624440
申请日：2020-07-01
Applicant: THE JOHNS HOPKINS UNIVERSITY
IPC: B01L3/00 , C12Q1/6858
Abstract: Provided herein are microfluidic devices for analyzing samples. In one aspect, the microfluidic device includes a body structure having a droplet compression chamber, a sieve structure in fluid communication with the droplet compression chamber, which sieve structure comprises an array of protrusions that extend from at least one surface of the body structure and define at least a portion of one or more fluidic circuits, and a port at least partially disposed in the body structure. Other aspects include kits, methods, systems, computer readable media, and related aspects for analyzing samples.
