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1.发明授权公开(公告)号：US08080423B2
公开(公告)日：2011-12-20
申请号：US11573179
申请日：2005-08-04
申请人： Takuji Kouzuma , Yoko Nagai , Shigeyuki Imamura
发明人： Takuji Kouzuma , Yoko Nagai , Shigeyuki Imamura
IPC分类号： G01N33/72
CPC分类号： G01N33/723 , C12N9/96 , C12Q1/37 , G01N2333/95 , G01N2333/964 , Y10T436/108331 , Y10T436/25 , Y10T436/2525
摘要： Stabilizers for colorants used for detection of enzymatic reactions, having a cyclodextrin and a catalase; reagents including stabilizers; and methods of use of such stabilizers and reagents.
摘要翻译： 用于检测酶反应的着色剂的稳定剂，具有环糊精和过氧化氢酶; 试剂包括稳定剂; 以及使用这种稳定剂和试剂的方法。
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2.发明申请公开(公告)号：US20070224685A1
公开(公告)日：2007-09-27
申请号：US11573179
申请日：2005-08-04
申请人： Takuji Kouzuma , Yoko Nagai , Shigeyuki Imamura
发明人： Takuji Kouzuma , Yoko Nagai , Shigeyuki Imamura
CPC分类号： G01N33/723 , C12N9/96 , C12Q1/37 , G01N2333/95 , G01N2333/964 , Y10T436/108331 , Y10T436/25 , Y10T436/2525
摘要： Provided are: a protease reaction promoter which is superior in usefulness to the known protease reaction promoters; a stabilizer for a colorant used for detection of an enzymatic reaction, and a method of stabilizing the colorant; and a reagent for and a method of measuring a glycated protein and a ratio of a glycated protein using the same. There are provided: a protease reaction promoter which contains at least one kind selected from the group consisting of an acetate group-containing compound or a salt thereof, N-acyl taurine or a salt thereof, and a polyoxyethylene alkyl ether sulfuric acid or a salt thereof; a method of promoting a protease reaction by using the protease reaction promoter; a stabilizer reagent for stabilizing a colorant used for detection of an enzymatic reaction, which contains a cyclodextrin; a stabilization method, including allowing a cyclodextrin to coexist with the colorant; and a reagent for and a method of measuring a glycated protein and a ratio of a glycated protein by using the protease reaction promoter and/or the reagent containing a cyclodextrin.
摘要翻译： 提供：对已知的蛋白酶反应促进剂有用的蛋白酶反应促进剂; 用于检测酶反应的着色剂的稳定剂和稳定着色剂的方法; 以及测定糖化蛋白质的试剂和测定方法以及使用其的糖化蛋白质的比例。 提供：含有选自含有乙酸基的化合物或其盐，N-酰基牛磺酸或其盐和聚氧乙烯烷基醚硫酸或其盐中的至少一种的蛋白酶反应促进剂 的; 通过使用蛋白酶反应促进剂促进蛋白酶反应的方法; 用于稳定用于检测酶反应的着色剂的稳定剂试剂，其含有环糊精; 包括使环糊精与着色剂共存的稳定化方法; 以及通过使用蛋白酶反应促进剂和/或含有环糊精的试剂测定糖化蛋白质和糖化蛋白质的比例的试剂和方法。
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公开(公告)号：US20050101771A1
公开(公告)日：2005-05-12
申请号：US10470678
申请日：2002-01-30
IPC分类号： C12Q1/37 , G01N33/68 , G01N33/53 , C07K14/37 , G01N33/569
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）使蛋白酶和至少起作用于糖基化氨基酸的酶的稳定化; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US20110312009A1
公开(公告)日：2011-12-22
申请号：US11831783
申请日：2007-07-31
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) stabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）稳定蛋白酶和至少作用于糖化氨基酸的酶; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US07250269B2
公开(公告)日：2007-07-31
申请号：US10470678
申请日：2002-01-30
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）使蛋白酶和至少起作用于糖基化氨基酸的酶的稳定化; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US20120129202A1
公开(公告)日：2012-05-24
申请号：US13316404
申请日：2011-12-09
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) stabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下步骤准确测定糖化蛋白质的组合物：1）避免球蛋白和抗坏血酸组分的影响，2）稳定蛋白酶和至少作用于糖化氨基酸的酶; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US08105800B2
公开(公告)日：2012-01-31
申请号：US11831783
申请日：2007-07-31
CPC分类号： G01N33/6842 , C12Q1/37 , G01N33/68
摘要： Composition for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.
摘要翻译： 用于通过以下方式准确测定糖化蛋白质的组成：1）避免球蛋白和抗坏血酸组分的影响，2）使蛋白酶和至少作用于糖基化氨基酸的酶的稳定化; 3）准确测定白蛋白; 和4）测定糖化白蛋白同时避免糖化血红蛋白的影响，并提供测定方法。 因此，可以更准确地测定糖化蛋白质和糖化白蛋白的含量。
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公开(公告)号：US4770997A
公开(公告)日：1988-09-13
申请号：US820011
申请日：1986-01-21
CPC分类号： C12Y103/03005 , C12N9/001 , C12Q1/26 , Y10S435/836
摘要： Thermostable bilirubin oxidase having substrate specificity to at least bilirubin and capable of catalyzing a reaction in which biliverdin and water are formed from bilirubin and oxygen. It can be produced by culturing a bilirubin oxidase producing micro-organism belonging to the genus Bacillus, for example, Bacillus licheniformis and then preparing the resultant bilirubin oxidase from the cultured broth.
摘要翻译： 具有对至少胆红素具有底物特异性并且能够催化由胆红素和氧形成胆碱和水的反应的热稳定性胆红素氧化酶。 可以通过培养属于芽孢杆菌属的例如地衣芽孢杆菌的产生胆红素氧化酶的微生物，然后从培养的肉汤中制备所得到的胆红素氧化酶来制备。
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公开(公告)号：US4525458A
公开(公告)日：1985-06-25
申请号：US413677
申请日：1982-09-01
申请人： Shigeyuki Imamura , Hideo Misaki , Naoki Muto
发明人： Shigeyuki Imamura , Hideo Misaki , Naoki Muto
CPC分类号： C12N9/16 , Y10S435/886
摘要： The invention is to provide a method for the production of sphingomyelinase, by culturing in a medium a sphingomyelinase-producing strain belonging to genus Streptomyces and collecting the resulting sphingomyelinase from the cultured medium. The strain, Streptomyces sp. A 9107, newly separated from a soil, is found preferable for the method of the present invention, and has been deposited as NRRL 15100 and FERM-P 4978.
摘要翻译： 本发明提供一种通过在培养基中培养属于链霉菌属的产生鞘磷脂酶的菌株并从培养基中收集得到的鞘磷脂酶来提供鞘磷脂酶的生产方法。 菌株，链霉菌属 对于本发明的方法，优选新的与土壤分离的9107，并且已经保藏为NRRL 15100和FERM-P 4978。
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公开(公告)号：US4346173A
公开(公告)日：1982-08-24
申请号：US201123
申请日：1980-10-27
申请人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
发明人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
IPC分类号： C12N9/02
CPC分类号： C12Y103/03006 , C12N9/001 , C12R1/06 , C12R1/645 , C12R1/66 , C12R1/865 , Y10S435/83 , Y10S435/911 , Y10S435/913 , Y10S435/942
摘要： A process for the production of acyl-Coenzyme A oxidase, comprises culturing an acyl-Coenzyme A-oxidase-producing microorganism belonging to genus Macrophomina, genus Cladosporium, genus Aspergillus, genus Monascus, genus Saccharomyces or genus Arthrobacter in a nutrient medium, and isolating the thus-formed acyl-CoA oxidase therefrom. The preferred species of microorganism are Macrophomina phaseoli ATCC 14383, Cladosporium resinae IFO 6367, Aspergillus candidus M-4815 FERM-P No. 5226, Monascus sp. M-4800 FERM-P No. 5225, Saccharomyces cerevisiae Y 0036 FERM-P No. 5174, and Arthrobacter sp. B-720 FERM-P No. 5224, respectively.
摘要翻译： 一种制备酰基辅酶A氧化酶的方法，包括在营养培养基中培养属于巨噬细胞，枝孢霉属，曲霉属，红曲霉属，酿酒酵母属或节杆菌属的酰基辅酶A氧化酶微生物， 由此形成的酰基辅酶A氧化酶。 微生物的优选种类是Macrophomina phaseoli ATCC 14383，Cladosporium resinae IFO 6367，Aspergillus candidus M-4815 FERM-P No.5226，Monascus sp。 M-4800 FERM-P No.5225，酿酒酵母Y 0036 FERM-P No.5174和节杆菌属（Arthrobacter sp。 B-720 FERM-P No. 5224。
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