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    Process for producing cis-3-hydroxy-L-proline
    5.
    发明授权
    Process for producing cis-3-hydroxy-L-proline 失效
    顺式-3-羟基-L-脯氨酸的制备方法

    公开(公告)号：US06767726B2

    公开(公告)日：2004-07-27

    申请号：US10138632

    申请日：2002-05-06

    申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

    发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

    IPC分类号： C12N902

    CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

    摘要： An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.

    摘要翻译： 分离的基因编码具有SEQ ID NO：1,2,15,16和17的氨基酸序列的L-脯氨酸-3-羟化酶，或编码具有酶活性的蛋白质，以使L-脯氨酸的3-位羟基化 并在2-酮戊二酸和二价铁离子存在下作用于游离的L-脯氨酸以产生顺式-3-羟基-L-脯氨酸。 将该基因插入载体中，并将​​所得的重组DNA用于产生转化体。 通过在培养基中培养转化体产生L-脯氨酸-3-羟化酶。

    Process for producing Cis-3-hydroxy-L-proline
    7.
    发明授权
    Process for producing Cis-3-hydroxy-L-proline 有权
    生产Cis-3-羟基-L-脯氨酸的方法

    公开(公告)号：US6010891A

    公开(公告)日：2000-01-04

    申请号：US287375

    申请日：1999-04-07

    申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

    发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Keiko Ochiai ,  Shigeru Chiba ,  Yoichi Uosaki

    IPC分类号： C12N9/02 ,  C12P13/24 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002 ,  Y10S435/814

    摘要： An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant, is also provided.

    摘要翻译： 用于生产顺式-3-羟基-L-脯氨酸的工业上适用的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于链霉菌属或芽孢杆菌属的微生物的酶源存在下转化为顺式-3-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化为顺式 - 3-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，在水性介质中收集生成的顺式-3-羟基-L-脯氨酸。 一种新型的L-脯氨酸-3-羟化酶，可用于该方法的L-脯氨酸-3-羟化酶的基因，含该基因的转化体，以及使用该转化体产生L-脯氨酸-3-羟化酶的方法， 也提供。

    Process for producing trans-4-hydroxy-L-proline
    9.
    发明授权
    Process for producing trans-4-hydroxy-L-proline 失效
    制备反式-4-羟基-L-脯氨酸的方法

    公开(公告)号：US07238501B2

    公开(公告)日：2007-07-03

    申请号：US10610561

    申请日：2003-07-02

    申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

    发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

    IPC分类号： C12P13/24

    CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

    摘要： The present invention is directed to a process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. In addition, the present invention is directed to a process for producing trans-4-hydroxy-L-proline, wherein the L-proline biosynthesis activity of the host cell of the transformant is reinforced.

    摘要翻译： 本发明涉及一种生产反式-4-羟基-L-脯氨酸的方法，其可用作药物的原料或作为食品添加剂。 在此过程中，L-脯氨酸在衍生自属于Dactylosporangium，Amycolatopsis或Streptomyces属的微生物的酶源的存在下转化成反式-4-羟基-L-脯氨酸，其催化L-脯氨酸的羟基化 在水性介质中转化成反式-4-羟基-L-脯氨酸，二价铁离子和2-酮戊二酸，从水性介质中收集生成的反式-4-羟基-L-脯氨酸。 此外，本发明涉及一种生产反式-4-羟基-L-脯氨酸的方法，其中转化体宿主细胞的L-脯氨酸生物合成活性得到增强。

    Process for producing trans-4-hydroxy-L-proline
    10.
    发明授权
    Process for producing trans-4-hydroxy-L-proline 失效

    公开(公告)号：US06617140B2

    公开(公告)日：2003-09-09

    申请号：US09833555

    申请日：2001-04-13

    申请人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

    发明人： Akio Ozaki ,  Hideo Mori ,  Takeshi Shibasaki ,  Katsuhiko Ando ,  Shigeru Chiba

    IPC分类号： C12P1324

    CPC分类号： C12N9/0071 ,  C12P13/24 ,  C12Y114/11002

    摘要： Provided is an industrially applicable process for producing trans-4-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into trans-4-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Dactylosporangium, Amycolatopsis or Streptomyces and which catalyzes the hydroxylation of L-proline into trans-4-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced trans-4-hydroxy-L-proline is collected from the aqueous medium. Also provided is a novel enzyme L-proline-4-hydroxylase which is useful for the process, a gene of L-proline-4-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-4-hydroxylase using the transformant. In addition, provided is a process for producing L-proline-4-hydroxylase using the transformant which contains the gene and has a reinforced proline biosynthesis activity.