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8 条记录 (耗时 0.029 秒)

    Purified cyclodextrinase
    1.
    发明授权
    Purified cyclodextrinase 失效
    纯化CYCLODEXTRINASE

    公开(公告)号：US5110734A

    公开(公告)日：1992-05-05

    申请号：US533856

    申请日：1990-06-06

    申请人： Tetsuya Oguma ,  Mamoru Kikuchi ,  Kiyoshi Mizusawa ,  Shoichi Tokutake ,  Nobuyuki Yamaji

    发明人： Tetsuya Oguma ,  Mamoru Kikuchi ,  Kiyoshi Mizusawa ,  Shoichi Tokutake ,  Nobuyuki Yamaji

    IPC分类号： C12N9/24 ,  C12P19/14

    CPC分类号： C12Y302/01054 ,  C12N9/2402 ,  C12P19/14

    摘要： The present invention relates to a novel cyclodextrinase having the specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular, weight. This novel enzyme can be produced by culturing a specified microorganism belonging to the genus Bacillus. The invention also provides a process for preparing maltooligosaccharides by acting the present enzyme to cyclodextrins.

    摘要翻译： 本发明涉及具有与作用，底物特异性，最佳pH，稳定pH，抑制，活化和分子重量有关的特定物理化学特性的新型环糊精酶。 这种新型酶可以通过培养属于芽孢杆菌属的特定微生物来产生。 本发明还提供了通过将本发明酶作用于环糊精来制备麦芽低聚糖的方法。

    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter
    5.
    发明授权
    DNA fragment containing a tannase gene, a recombinant plasmid, a process for producing tannase, and a promoter 失效
    含有鞣酸酶基因的DNA片段，重组质粒，鞣酸酶的制备方法和启动子

    公开(公告)号：US5665584A

    公开(公告)日：1997-09-09

    申请号：US460860

    申请日：1995-06-05

    申请人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    发明人： Osamu Hatamoto ,  Teruo Watarai ,  Kiyoshi Mizusawa ,  Eiichi Nakano

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N9/18 ,  C12N15/00 ,  C12R1/69

    CPC分类号： C12N9/18

    摘要： The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.

    摘要翻译： 本发明涉及含有编码鞣酸酶的基因并衍生自属于曲霉属的微生物的3,563个碱基对的DNA片段，其具有以下限制酶图：B：Bam HI，H：Hind III，K ：Kpn I，S：Sal I，X：Xba I; 含有编码（SEQ ID NO：4）的氨基酸序列的鞣酸酶基因的DNA片段; 包含插入质粒载体中的含有鞣酸酶基因的DNA片段的重组质粒; 一种鞣酸酶的制造方法，其特征在于，在培养基中培养能够在培养基中生产鞣酸酶的微生物属于属于曲霉属的微生物，从培养物中回收鞣酸酶; 和由（SEQ ID NO：1）的核苷酸序列表示的启动子。 根据本发明可以有效地制备鞣酸酶。

    Monomethylamine-oxidizing enzyme
    6.
    发明授权
    Monomethylamine-oxidizing enzyme 失效
    单甲胺氧化酶

    公开(公告)号：US4503147A

    公开(公告)日：1985-03-05

    申请号：US435316

    申请日：1982-10-19

    申请人： Motoo Nakajima ,  Kazuo Nakamura ,  Yoshio Shirokane ,  Kiyoshi Mizusawa

    发明人： Motoo Nakajima ,  Kazuo Nakamura ,  Yoshio Shirokane ,  Kiyoshi Mizusawa

    IPC分类号： C12N9/06 ,  C12Q1/26 ,  C12R1/05 ,  C12R1/07

    CPC分类号： C12N9/0022 ,  C12Q1/26 ,  Y10S435/829 ,  Y10S435/832

    摘要： A monomethylamine-oxidizing enzyme can be obtained by cultivating in a medium a strain which belongs to Genus Bacillus and has an ability to produce a monomethylamine-oxidizing enzyme. This enzyme exhibits several beneficial properties including the ability to oxidatively deaminate the amino group of monomethylamine to produce formaldehyde, ammonia, and hydrogen peroxide. The enzyme exhibits a high substrate specificity for monomethylamine, ethylamine, and n-proplyamine while showing no substrate specificity for benzylamine, dimethylamine, trimethylamine, ethylenediamine and tryamine. In addition, the enzyme is stable through an elevated temperature range permitting faster reaction rates and therefore a shorter overall quantitative evaluation. Another property includes a low Km value which allows smaller quantities of the enzyme to be employed per sample.

    摘要翻译： 通过在培养基中培养属于芽孢杆菌属的菌株并具有产生单甲胺氧化酶的能力，可以获得单甲胺氧化酶。 该酶显示出几种有益的性质，包括使一甲胺的氨基氧化脱氨以产生甲醛，氨和过氧化氢的能力。 该酶对单甲胺，乙胺和正丙胺显示出高的底物特异性，同时对苄胺，二甲胺，三甲胺，乙二胺和试胺没有底物特异性。 此外，酶在升高的温度范围内是稳定的，允许更快的反应速率，因此较短的总体定量评估。 另一种性质包括低Km值，其允许每个样品使用较少量的酶。