公开(公告)号：US5364936A

公开(公告)日：1994-11-15

申请号：US174596

申请日：1993-12-28

申请人： Tetsuya Oguma ,  Tatsuo Horiuchi ,  Koichiro Tobe

发明人： Tetsuya Oguma ,  Tatsuo Horiuchi ,  Koichiro Tobe

IPC分类号： C08B37/02 ,  C12N9/44 ,  C12P19/18 ,  C08B37/16

CPC分类号： C12N9/2451 ,  C08B37/0021 ,  C12P19/18

摘要： The present invention relates to a novel cycloisomaltooligosaccharide selected from the group consisting of novel cycloisomaltoheptaose having a cyclic structure composed of 7 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooctaose having a cyclic structure composed of 8 glucose residues in .alpha.-1,6 linkage and novel cycloisomaltononaose having a cyclic structure composed of 9 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooligosaccharide synthase forming said oligosaccharides from dextran, and a process for producing said oligosaccharides by use of said enzyme or a microorganism capable of producing said enzyme.

摘要翻译： 本发明涉及一种选自下组的新型环状半缩水甘露寡糖，其由具有由α-1,6键连接的7个葡萄糖残基组成的环状结构的新型环异麦芽糖糖，具有由α-1,6中的8个葡萄糖残基组成的环状结构的新型环状体细菌 连接和具有由α-1,6键连接的9个葡萄糖残基组成的环状结构的新型环状马耳骨霉素，以及由右旋糖酐形成所述寡糖的新型环状二缩水甘露糖合成酶，以及通过使用所述酶或能够产生所述酶的微生物来生产所述寡糖的方法 。

公开(公告)号：US5453369A

公开(公告)日：1995-09-26

申请号：US284318

申请日：1994-08-02

申请人： Tetsuya Oguma ,  Tatsuo Horiuchi ,  Koichiro Tobe

发明人： Tetsuya Oguma ,  Tatsuo Horiuchi ,  Koichiro Tobe

IPC分类号： C08B37/02 ,  C12N9/44 ,  C12P19/18 ,  C12N9/10 ,  C12N9/24

CPC分类号： C12N9/2451 ,  C08B37/0021 ,  C12P19/18

摘要： The present invention relates to a novel cycloisomaltooligosaccharide selected from the group consisting of novel cycloisomaltoheptaose having a cyclic structure composed of 7 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooctaose having a cyclic structure composed of 8 glucose residues in .alpha.-1,6 linkage and novel cycloisomaltononaose having a cyclic structure composed of 9 glucose residues in .alpha.-1,6 linkage, novel cycloisomaltooligosaccharide synthase forming said oligosaccharides from dextran, and a process for producing said oligosaccharides by use of said enzyme or a microorganism capable of producing said enzyme.

摘要翻译： 本发明涉及一种选自下组的新型环状半缩水甘露寡糖，其由具有由α-1,6键连接的7个葡萄糖残基组成的环状结构的新型环异麦芽糖糖，具有由α-1,6中的8个葡萄糖残基组成的环状结构的新型环状体细菌 连接和具有由α-1,6键连接的9个葡萄糖残基组成的环状结构的新型环状马耳骨霉素，以及由右旋糖酐形成所述寡糖的新型环状二缩水甘露糖合成酶，以及通过使用所述酶或能够产生所述酶的微生物来生产所述寡糖的方法 。

公开(公告)号：US4395487A

公开(公告)日：1983-07-26

申请号：US256194

申请日：1981-04-21

申请人： Narimasa Saito ,  Tatsuo Horiuchi

发明人： Narimasa Saito ,  Tatsuo Horiuchi

IPC分类号： C12Q1/40

CPC分类号： C12Q1/40

摘要： A method for the assay of .alpha.-amylase activity, which comprises adding an .alpha.-amylase-containing sample to maltohexaitol or maltohexaonic acid used as substrate, reacting, at the same time or subsequent to the addition, .alpha.-glucosidase with the resulting mixture, and determining the reaction product to assay the .alpha.-amylase activity.

摘要翻译： 一种测定α-淀粉酶活性的方法，其包括将含有α-淀粉酶的样品添加到用作底物的麦芽六糖醇或麦芽六糖酸中，同时或之后加入α-葡糖苷酶与所得混合物， 并测定反应产物以测定α-淀粉酶活性。

公开(公告)号：US5260197A

公开(公告)日：1993-11-09

申请号：US407148

申请日：1989-09-14

申请人： Tatsuo Horiuchi ,  Minoru Hiruma ,  Toshiyuki Suzuki

发明人： Tatsuo Horiuchi ,  Minoru Hiruma ,  Toshiyuki Suzuki

IPC分类号： C12N9/04 ,  C12Q1/26 ,  C12Q1/32 ,  C12R1/38

CPC分类号： C12N9/0006 ,  C12Q1/32

摘要： L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2):(1) Action and substrate specificityIt withdraws hydrogen from L-fucose and converts into L-fuconolactone and at the same time, reduces coenzyme NADP.sup.+ to NADPH.(2) pH and stable pH rangeWhen Tris-imidazole-sodium acetate buffer solution is used, the optimum pH is in a range of 9.0 to 10.0 and the stable pH range is between 8.0 and 10.5.The L-fucose dehydrogenase utilizing NADP.sup.+ as coenzyme can be obtained by culturing L-fucose dehydrogenase-producing strain belonging to the genus Pseudomonas in a medium and collecting formed L-fucose dehydrogenase from the culture.L-Fucose can be quantitatively determined by reacting a sample containing L-fucose with L-fucose dehydrogenase requiring NADP.sup.+ as coenzyme and assaying NADPH produced.

摘要翻译： 具有以下物理化学性质（1）和（2）的L-岩藻糖脱氢酶：（1）作用和底物特异性从L-岩藻糖中提取氢并转化为L- uconolactone，同时将辅酶NADP +还原成NADPH。 （2）pH和稳定的pH范围当使用三咪唑 - 乙酸钠缓冲溶液时，最佳pH在9.0至10.0的范围内，稳定的pH范围在8.0和10.5之间。 利用NADP +作为辅酶的L-岩藻糖脱氢酶可以通过在培养基中培养属于假单胞菌属的L-岩藻糖脱氢酶生产菌株并从培养物收集形成的L-岩藻糖脱氢酶而获得。 可以通过使含有L-岩藻糖的样品与需要NADP +的L-岩藻糖脱氢酶作为辅酶并测定产生的NADPH来定量测定L-岩藻糖。

公开(公告)号：US4621057A

公开(公告)日：1986-11-04

申请号：US580243

申请日：1984-02-15

申请人： Tatsuo Horiuchi

发明人： Tatsuo Horiuchi

IPC分类号： C12N9/06 ,  C12N9/04 ,  C12R1/38

CPC分类号： C12N9/0006 ,  Y10S435/874

摘要： An N-acetylhexosamine oxidase having the following physiochemical properties.(1) Action and specificity for substrateOxidizes N-acetylhexosamine in the presence of oxygen to form N-acetylhexosaminic acid and hydrogen peroxide. Hardly acts or does not act at all on hexose and hexosamine.(2) Optimum pH and stable pH rangeWhen a potassium phosphate buffer solution contining 0.1M glycine is used, an optimum pH is 7.5 to 8.5 and a stable pH range is 3 to 9.(3) Molecular weightHas a molecular weight of about 140,000 to 150,000 when measured according to a gel filtration method using Sephadex G-200 by the use of 0.05M potassium phosphate buffer solution.

摘要翻译： 具有以下理化特性的N-乙酰基己糖胺氧化酶。 （1）底物的作用和特异性在氧气存在下氧化N-乙酰基己糖胺以形成N-乙酰基己糖胺酸和过氧化氢。 在己糖和己糖胺上几乎不起作用或不起作用。 （2）最佳pH值和稳定的pH范围当使用连续使用0.1M甘氨酸的磷酸钾缓冲溶液时，最适pH为7.5〜8.5，pH范围为3〜9。（3）分子量约为 根据使用Sephadex G-200通过使用0.05M磷酸钾缓冲溶液的凝胶过滤方法测量时，为140,000至150,000。

公开(公告)号：US06831995B1

公开(公告)日：2004-12-14

申请号：US09520842

申请日：2000-03-08

申请人： Toshio Asano ,  Kaoru Sakai ,  Jun Mochizuki ,  Tatsuo Horiuchi ,  Hirofumi Nakatoyodome ,  Tadashi Furukawa ,  Atsuo Ohsawa

发明人： Toshio Asano ,  Kaoru Sakai ,  Jun Mochizuki ,  Tatsuo Horiuchi ,  Hirofumi Nakatoyodome ,  Tadashi Furukawa ,  Atsuo Ohsawa

IPC分类号： G06K900

CPC分类号： G09G3/006 ,  G06T7/0004

摘要： In image-capture inspection of electronic displays, the object of the present invention is to reduce moire generation, improve inspection precision by making pixel defects easier to discover, and allowing quantitative evaluation of these pixel defects. The relative positioning of the electronic display and an imaging element is changed by very small amounts of 1/n of the pixel pitch. The n images are arranged by pixel and the images are combined by taking the moving average of the image data values to reduce the image-capture moires generated during the image capture operations. The composite image is used to inspect pixel defects in the electronic display.

摘要翻译： 在电子显示器的图像捕获检查中，本发明的目的是通过使像素缺陷更容易发现，并且允许对这些像素缺陷的定量评估来减少波纹产生，提高检查精度。电子显示器和 成像元件以非常少量的像素间距的1 / n改变。 n个图像由像素排列，并且通过拍摄图像数据值的移动平均来组合图像，以减少在图像捕获操作期间产生的图像捕获摩尔。 复合图像用于检查电子显示器中的像素缺陷。

公开(公告)号：US6166380A

公开(公告)日：2000-12-26

申请号：US70839

申请日：1998-05-01

申请人： Taiji Kitagawa ,  Mitsugu Sato ,  Goroku Shimoma ,  Tadanori Takahashi ,  Naoto Yoshida ,  Masayuki Yukii ,  Takanori Ninomiya ,  Tatsuo Horiuchi ,  Keisuke Kawame

发明人： Taiji Kitagawa ,  Mitsugu Sato ,  Goroku Shimoma ,  Tadanori Takahashi ,  Naoto Yoshida ,  Masayuki Yukii ,  Takanori Ninomiya ,  Tatsuo Horiuchi ,  Keisuke Kawame

IPC分类号： G01N1/00 ,  G01N1/28 ,  H01J37/22 ,  H01J37/28 ,  H01J37/26

CPC分类号： H01J37/28 ,  H01J2237/2823

摘要： An electron microscope resolving power evaluation method for evaluating performance of a scanning electron microscope without resorting to visual sensory analysis for minimizing a difference in deterioration with age and in performance among individual scanning electron microscopes. A specimen is prepared by overlaying materials having different emission coefficients of secondary charged particles such as secondary electrons, backscattered electrons, transmitted electrons, etc., a cross-section including an overlaid material part of thin-film layers having known dimensions is mirror-finished, data of a scanning electron microscopical image of the cross-section, including the overlaid material part is obtained, and then the resolving power performance of the scanning electron microscope is evaluated quantitatively by means of frequency analysis, etc.

摘要翻译： 一种用于评估扫描电子显微镜的性能的电子显微镜分辨能力评估方法，而不用通过视觉感官分析来最小化各个扫描电子显微镜之间的年龄和性能差异的差异。 通过覆盖具有二次电荷，后向散射电子，透射电子等二次带电粒子的不同发射系数的材料制备样品，包括具有已知尺寸的薄膜层的重叠部分的横截面是镜面加工 得到包括重叠材料部分的横截面的扫描电子显微镜图像的数据，然后通过频率分析等定量评估扫描电子显微镜的分辨能力性能。

公开(公告)号：US5942427A

公开(公告)日：1999-08-24

申请号：US637865

申请日：1990-12-27

申请人： Hideko Otake ,  Yasuji Koyama ,  Tatsuo Horiuchi ,  Eiichi Nakano

发明人： Hideko Otake ,  Yasuji Koyama ,  Tatsuo Horiuchi ,  Eiichi Nakano

IPC分类号： C12N9/04 ,  C12N15/09 ,  C12N15/53 ,  C12R1/01 ,  C12R1/19 ,  C12R1/20 ,  C12N15/70

CPC分类号： C12N9/0006

摘要： N-acetylmannosamine dehydrogenase gene derived from a microorganism belonging to the genus Flavobacterium, e.g., Flavobacterium sp. No. 141-8 strain and defined by a specific restriction enzyme map which encodes 271 amino acid sequence. Using the recombinant DNA, N-acetylmannosamine dehydrogenase can be produced in a simpler manner in an industrial scale. The enzyme is useful for quantitative determination of sialic acid.

摘要翻译： 衍生自属于黄杆菌属的微生物的N-乙酰甘露糖胺脱氢酶基因，例如黄杆菌属 通过编码271个氨基酸序列的特异性限制酶图谱定义。 使用重组DNA，N-乙酰甘露糖胺脱氢酶可以以工业规模更简单的方式生产。 该酶可用于定量测定唾液酸。

公开(公告)号：US4443836A

公开(公告)日：1984-04-17

申请号：US363514

申请日：1982-03-30

申请人： Tatsuo Horiuchi ,  Kazushige Kiyota

发明人： Tatsuo Horiuchi ,  Kazushige Kiyota

IPC分类号： B60Q1/05 ,  B60Q1/18 ,  F21V15/00 ,  F21V17/00

CPC分类号： B60Q1/18 ,  B60Q1/05

摘要： The invention discloses an auxiliary head lamp device for a car equipped with a cover which is optionally fitted to a car and is lit during driving in fog or the like. The cover of this device can be opened when a solenoid of a cover driving mechanism is energized by operating a switch disposed inside the room of the car. The solenoid releases engagement of a stopper that restricts the cover urged in the opening direction. The switch may be used also as a switch for a light source bulb. After the solenoid is energized and the opening operation of the cover starts, a current to the solenoid is automatically cut off so that over-heat or burn-out of the solenoid that would occur if the current is continuously applied thereto can be prevented.

摘要翻译： 本发明公开了一种用于车辆的辅助头灯装置，其配备有可选地安装在汽车上的盖子，并且在雾中驾驶期间点亮等。 当通过操作设置在汽车的房间内的开关来给盖驱动机构的螺线管通电时，可以打开该装置的盖。 螺线管释放限制沿打开方向推动的盖的止动器的接合。 该开关也可以用作光源灯泡的开关。 在螺线管通电并且盖子的打开操作开始之后，自动切断到螺线管的电流，从而可以防止如果电流被连续地施加到电磁阀时会发生的螺线管的过热或烧坏。

公开(公告)号：US6054305A

公开(公告)日：2000-04-25

申请号：US941936

申请日：1997-10-01

申请人： Hiroki Tatsumi ,  Naoki Eisaki ,  Tatsuo Horiuchi ,  Ayumu Nagahara

发明人： Hiroki Tatsumi ,  Naoki Eisaki ,  Tatsuo Horiuchi ,  Ayumu Nagahara

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/48 ,  C12R1/19 ,  C12N15/63

CPC分类号： C12N9/1294 ,  C12Q1/48 ,  G01N2333/91295

摘要： Disclosed are a pyruvate orthophosphate dikinase gene coding for a polypeptide containing the amino acid sequence of SEQ ID NO:1 or an amino acid sequence where in said amino acid sequence one or more amino acids are added, deleted or substituted and bringing about the activity of pyruvate orthophosphate dikinase; recombinant DNA having the pyruvate orthophosphate dikinase gene integrated in vector DNA; and a process for producing pyruvate orthophosphate dikinase, which comprises culturing a microorganism belonging to the genus Escherichia carrying the above recombinant DNA in a medium, and recovering pyruvate orthophosphate dikinase from the resulting culture. The pyruvate orthophosphate dikinase gene and a process for producing pyruvate orthophosphate dikinase are provided.

摘要翻译： 公开了编码含有SEQ ID NO：1的氨基酸序列的多肽的丙酮酸正磷酸二激酶基因或氨基酸序列，其中在所述氨基酸序列中添加一个或多个氨基酸，缺失或取代并产生 丙酮酸正磷酸二激酶; 具有整合在载体DNA中的丙酮酸正磷酸二激酶基因的重组DNA; 以及生产丙酮酸正磷酸二激酶的方法，其包括在培养基中培养属于携带上述重组DNA的埃希氏杆菌属的微生物，并从所得培养物中回收丙酮酸正磷酸二激酶。 提供丙酮酸正磷酸二激酶基因和制备丙酮酸正磷酸二激酶的方法。