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1.发明申请Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression 审中-公开宿主细胞和使用抑制子多肽和诱导型启动子进行基因表达的方法公开(公告)号：US20170002363A1
公开(公告)日：2017-01-05
申请号：US15201248
申请日：2016-07-01
发明人： Thomas L. Ruegg
IPC分类号： C12N15/63 , C07K14/245
CPC分类号： C07K14/245 , C07K14/70567 , C12N15/63
摘要： The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
摘要翻译： 本发明提供了一种系统，其包含（a）第一核酸，其包含编码与包含阻遏物多肽结合位点的启动子可操作地连接的目的核苷酸序列的核苷酸序列，和（b）第二核酸，其包含编码 与EilR，SmvR，KmrR，RcdA或QacR具有至少70％氨基酸同一性的阻遏物多肽; 通过存在疏水性诱导剂，例如疏水性阳离子诱导剂，例如三芳基甲烷，吖啶，吩嗪，吩噻嗪或呫吨，可以诱导来自启动子的目标核苷酸序列的表达。
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2.发明授权公开(公告)号：US11299519B2
公开(公告)日：2022-04-12
申请号：US16831649
申请日：2020-03-26
发明人： Thomas L. Ruegg
IPC分类号： C07K14/245 , C12N15/63
摘要： The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
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3.发明申请公开(公告)号：US20200262875A1
公开(公告)日：2020-08-20
申请号：US16831649
申请日：2020-03-26
发明人： Thomas L. Ruegg
IPC分类号： C07K14/245 , C12N15/63
摘要： The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
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