公开(公告)号：US09814802B2

公开(公告)日：2017-11-14

申请号：US13874054

申请日：2013-04-30

申请人： The University of Kansas ,  The Children's Mercy Hospital

发明人： Omar Aljitawi ,  Richard Hopkins ,  Michael Detamore ,  Rama Garimella

IPC分类号： A61L27/36 ,  A61L27/38

CPC分类号： A61L27/3604 ,  A61L27/3683 ,  A61L27/3804 ,  A61L2430/18

摘要： A method of differentiating cells into CK19-positive cells capable of producing hair follicle-like and hair structure-like can include: providing a tissue scaffold; seeding cells into the scaffold, the cells being capable of differentiation; incubating the scaffold having the cells in a cell growth media; and incubating the scaffold having the cells in an osteogenic differentiation medium sufficient for CK19-positive cells to be generated in the scaffold. The tissue scaffold can be a decellularized Whartons' jelly matrix. The cell growth media excludes osteogenic differentiation components: dexamethasone, β-glycerophosphate, 1α,25-hydroxyvitamin D3, and ascorbic acid 2-phosphate. The osteogenic differentiation medium includes the osteogenic differentiation components. The cells can be mesenchymal cells, such as WJMSCs.

公开(公告)号：US20140148915A1

公开(公告)日：2014-05-29

申请号：US13874054

申请日：2013-04-30

申请人： The University of Kansas ,  The Children's Mercy Hospital

发明人： Omar Aljitawi ,  Richard Hopkins ,  Michael Detamore ,  Rama Garimella

IPC分类号： A61L27/36

CPC分类号： A61L27/3604 ,  A61L27/3683 ,  A61L27/3804 ,  A61L2430/18

摘要： A method of differentiating cells into CK19-positive cells capable of producing hair follicle-like and hair structure-like can include: providing a tissue scaffold; seeding cells into the scaffold, the cells being capable of differentiation; incubating the scaffold having the cells in a cell growth media; and incubating the scaffold having the cells in an osteogenic differentiation medium sufficient for CK19-positive cells to be generated in the scaffold. The tissue scaffold can be a decellularized Whartons' jelly matrix. The cell growth media excludes osteogenic differentiation components: dexamethasone, β-glycerophosphate, 1α,25-hydroxyvitamin D3, and ascorbic acid 2-phosphate. The osteogenic differentiation medium includes the osteogenic differentiation components. The cells can be mesenchymal cells, such as WJMSCs.

摘要翻译： 将细胞分化成能够产生毛囊样和毛发结构的CK19阳性细胞的方法可以包括：提供组织支架; 将细胞接种到支架中，细胞能够分化; 将具有细胞的支架在细胞生长培养基中孵育; 并将具有细胞的支架在成骨分化培养基中孵育，足以使CK19阳性细胞在支架中产生。 组织支架可以是脱细胞的沃顿饮料基质。 细胞生长培养基排除成骨分化成分：地塞米松，甘油磷酸酯，1α，25-羟基维生素D3和2-磷酸抗坏血酸。 成骨分化培养基包括成骨分化成分。 细胞可以是间充质细胞，如WJMSCs。