公开(公告)号：US07256008B2

公开(公告)日：2007-08-14

申请号：US11327711

申请日：2006-01-06

申请人： Thomas G. Spring ,  Elaine M. Brate ,  Shelley Holets-McCormack ,  Rajarathnam E. Reddy ,  Donald D. Johnson ,  Yon-Yih Chen ,  You Pan

发明人： Thomas G. Spring ,  Elaine M. Brate ,  Shelley Holets-McCormack ,  Rajarathnam E. Reddy ,  Donald D. Johnson ,  Yon-Yih Chen ,  You Pan

IPC分类号： G01N33/00

CPC分类号： G01N33/9493

摘要： Methods and kits for measurement of concentration of FK778 in a biological sample by means of an immunoassay, preferably a competitive immunoassay. In one aspect, the method and kit involve the use of (a) an antibody to FK778 conjugated to a label, e.g., an acridinium label, (b) an antibody to FK778 not conjugated to a label, (c) a solid phase containing an antibody to a first hapten, e.g., a fluorescein hapten, and (d) a bihapten comprising a first hapten and FK778 or an analogue of FK778, e.g., a bihapten comprising a fluorescein hapten and a FK778 hapten. In another aspect, the method and kit involve the use of (a) antibody to FK778, (b) a bihapten comprising FK778 or an analogue of FK778 and a first hapten, e.g., a bihapten comprising the fluorescein hapten and the hapten of FK778 or an analogue of FK778, and (c) a pretreatment reagent.

摘要翻译： 通过免疫测定，优选竞争性免疫测定法测量生物样品中FK778的浓度的方法和试剂盒。 一方面，该方法和试剂盒涉及使用（a）与标记缀合的FK778的抗体，例如吖啶标记，（b）与标记物不缀合的FK778的抗体，（c）含有 第一半抗原的抗体，例如荧光素半抗原，和（d）包含第一半抗原和FK778或FK778的类似物的双突变体，例如包含荧光素半抗原和FK778半抗原的双突变体。 另一方面，该方法和试剂盒涉及使用（a）FK778的抗体，（b）包含FK778或类似物FK778的双突变体和第一半抗原，例如包含荧光素半抗原的双突变体和FK778的半抗原或 FK778的类似物，（c）预处理试剂。

公开(公告)号：US20070269841A1

公开(公告)日：2007-11-22

申请号：US11770907

申请日：2007-06-29

申请人： Thomas Spring ,  Elaine Brate ,  Shelley Holets-McCormack ,  Rajarathnam Reddy ,  Donald Johnson ,  Yon-Yih Chen ,  You Pan

发明人： Thomas Spring ,  Elaine Brate ,  Shelley Holets-McCormack ,  Rajarathnam Reddy ,  Donald Johnson ,  Yon-Yih Chen ,  You Pan

IPC分类号： G01N33/00

CPC分类号： G01N33/9493

摘要： Methods and kits for measurement of concentration of FK778 in a biological sample by means of an immunoassay, preferably a competitive immunoassay. In one aspect, the method and kit involve the use of (a) an antibody to FK778 conjugated to a label, e.g., an acridinium label, (b) an antibody to FK778 not conjugated to a label, (c) a solid phase containing an antibody to a first hapten, e.g., a fluorescein hapten, and (d) a bihapten comprising a first hapten and FK778 or an analogue of FK778, e.g., a bihapten comprising a fluorescein hapten and a FK778 hapten. In another aspect, the method and kit involve the use of (a) antibody to FK778, (b) a bihapten comprising FK778 or an analogue of FK778 and a first hapten, e.g., a bihapten comprising the fluorescein hapten and the hapten of FK778 or an analogue of FK778, and (c) a pretreatment reagent.

摘要翻译： 通过免疫测定，优选竞争性免疫测定法测量生物样品中FK778的浓度的方法和试剂盒。 一方面，该方法和试剂盒涉及使用（a）与标记缀合的FK778的抗体，例如吖啶标记，（b）与标记物不缀合的FK778的抗体，（c）含有 第一半抗原的抗体，例如荧光素半抗原，和（d）包含第一半抗原和FK778或FK778的类似物的双突变体，例如包含荧光素半抗原和FK778半抗原的双突变体。 另一方面，该方法和试剂盒涉及使用（a）FK778的抗体，（b）包含FK778或类似物FK778的双突变体和第一半抗原，例如包含荧光素半抗原的双突变体和FK778的半抗原或 FK778的类似物，（c）预处理试剂。

公开(公告)号：US20070243631A1

公开(公告)日：2007-10-18

申请号：US11770853

申请日：2007-06-29

申请人： Thomas Spring ,  Elaine Brate ,  Shelley Holets-McCormack ,  Rajarathnam Reddy ,  Donald Johnson ,  Yon-Yih Chen ,  You Pan

发明人： Thomas Spring ,  Elaine Brate ,  Shelley Holets-McCormack ,  Rajarathnam Reddy ,  Donald Johnson ,  Yon-Yih Chen ,  You Pan

IPC分类号： G01N33/566

CPC分类号： G01N33/9493

摘要： Methods and kits for measurement of concentration of FK778 in a biological sample by means of an immunoassay, preferably a competitive immunoassay. In one aspect, the method and kit involve the use of (a) an antibody to FK778 conjugated to a label, e.g., an acridinium label, (b) an antibody to FK778 not conjugated to a label, (c) a solid phase containing an antibody to a first hapten, e.g., a fluorescein hapten, and (d) a bihapten comprising a first hapten and FK778 or an analogue of FK778, e.g., a bihapten comprising a fluorescein hapten and a FK778 hapten. In another aspect, the method and kit involve the use of (a) antibody to FK778, (b) a bihapten comprising FK778 or an analogue of FK778 and a first hapten, e.g., a bihapten comprising the fluorescein hapten and the hapten of FK778 or an analogue of FK778, and (c) a pretreatment reagent.

公开(公告)号：US20070161123A1

公开(公告)日：2007-07-12

申请号：US11327711

申请日：2006-01-06

申请人： Thomas Spring ,  Elaine Brate ,  Shelley Holets-McCormack ,  Rajarathnam Reddy ,  Donald Johnson ,  Yon-Yih Chen ,  You Pan

发明人： Thomas Spring ,  Elaine Brate ,  Shelley Holets-McCormack ,  Rajarathnam Reddy ,  Donald Johnson ,  Yon-Yih Chen ,  You Pan

IPC分类号： G01N33/543

CPC分类号： G01N33/9493

摘要： Methods and kits for measurement of concentration of FK778 in a biological sample by means of an immunoassay, preferably a competitive immunoassay. In one aspect, the method and kit involve the use of (a) an antibody to FK778 conjugated to a label, e.g., an acridinium label, (b) an antibody to FK778 not conjugated to a label, (c) a solid phase containing an antibody to a first hapten, e.g., a fluorescein hapten, and (d) a bihapten comprising a first hapten and FK778 or an analogue of FK778, e.g., a bihapten comprising a fluorescein hapten and a FK778 hapten. In another aspect, the method and kit involve the use of (a) antibody to FK778, (b) a bihapten comprising FK778 or an analogue of FK778 and a first hapten, e.g., a bihapten comprising the fluorescein hapten and the hapten of FK778 or an analogue of FK778, and (c) a pretreatment reagent.